Hibernate Medium is a CO2
-independent nutrient medium for the maintenance of neural cells, tissue, and tissue slices. Hibernate-A Medium is optimized for adult and postnatal cells or tissue in ambient carbon dioxide levels.
Hibernate Medium enables neurons to be maintained in ambient CO2
levels for up to 48 hours. This provides scientists with greater convenience and control while running bench-top live cell experiments, such as microscopy, flow cytometry, and other physiology studies. Hibernate Medium also allows for the preservation of viable brain tissue before processing for up to a month with refrigeration and maintenance of tissue slices.
Hibernate Medium may be combined with B-27 Supplements
to yield a complete medium.
For other applications, we provide alternative formulations of Hibernate Medium
and B-27 Supplements
For established protocols using Hibernate Medium, access our Gibco Neurobiology Protocol Handbook
View publications using Gibco neurobiology products at our Gibco CellCite
For Research Use Only. Not for use in diagnostic procedures.