BacMam p53 [AcLys382] Cellular Assay Kit

Catalog number: A12901

 Related applications: Oncology & Genetic Disease Research | Target & Lead Identification & Validation

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Description

The combination of baculovirus-mediated gene delivery (BacMam) with LanthaScreen® Cellular Assay technology enables a platform for the analysis of specific posttranslational modifications of various substrates including p53. BacMam provides a convenient genetic delivery tool for a GFP-p53 fusion protein in the cell line of interest. This kit describes an HTS-compatible cellular immunoassay measuring acetylation of GFP-p53 at Lys382.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

System: LanthaScreen®
Readout: End Point
Reporter: GFP (EmGFP)
Technique: TR-FRET
Cell State: Cells Not Included
Product Size: 1 kit
Detection Method: Fluorescent

Contents & storage

Kit contains Terbium-labeled anti-p53 antibody, BacMam p53 Virus, and 6X Lysis Buffer. Store as indicated in handling documentation.

Documents

Manuals & protocols