Invitrogen™

7-AAD (7-Aminoactinomycin D)

Catalog number: A1310
Invitrogen™

7-AAD (7-Aminoactinomycin D)

Catalog number: A1310
Catalog Number
A1310
Unit Size
1 mg
Price (USD)
Price: 196.00
Online price: 174.65
Your price:
Availability
-
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
A13101 mg
Price: 196.00
Online price: 174.65
Your price:
-
Product Overview
Figures
Videos
Recommendations
Recommendations
Documents
FAQ
Citations & References
Additional Information
Recommendations

7-aminoactinomycin D (7-AAD) is a fluorescent intercalator that undergoes a spectral shift upon association with DNA. 7-AAD/DNA complexes can be excited by the 488 nm laser and has an emission maxima of 647 nm, making this nucleic acid stain useful for multicolor fluorescence microscopy and flow cytometry. 7-AAD appears to be generally excluded from live cells, but can be used with cells that have been fixed and permeabilized. 7-AAD has been used for cell cycle analysis by flow cytometry. 7-AAD also binds selectively to GC regions of DNA yielding a distinct banding pattern in polytene chromosomes and chromatin for use in chromosome banding studies.

To make a concentrated stock solution from 1–2 mM, dissolve 7-AAD in either high quality anhydrous DMSO or DMF. Store frozen, desiccated and protected from light.

View a selection guide for all nonfixable viability dyes for flow cytometry.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Excitation Wavelength Range
647 nm
Dye Type
Cell-Impermeant
For Use With (Equipment)
Fluorescence Microscope, Flow Cytometer
Detection Method
Fluorescence
Emission
546 nm
Form
Solid
Shipping Condition
Room Temperature
Solubility
DMSO (Dimethylsulfoxide)
Product Type
Nuclear Labeling
SubCellular Localization
Nucleic Acids, Nucleus
Molecular Weight (g/mol)
1270.45

Contents & Storage

Contains 1 vial of 7-AAD (1 mg).

Store in freezer (-5°C to -30°C) and protect from light.

Figures

Documents & Downloads

Certificates

    Frequently asked questions (FAQs)

    Citations & References

    Search citations by name, author, journal title or abstract text