7-AAD (7-Aminoactinomycin D)

Catalog number: A1310

Molecular Probes™  Related applications: Cell Structure | Cell Tracing & Tracking | Cell Viability, Proliferation & Function | Cellular Imaging

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7-aminoactinomycin D (7-AAD) is a fluorescent intercalator that undergoes a spectral shift upon association with DNA. 7-AAD/DNA complexes can be excited by the 488 nm laser and has an emission maxima of 647 nm, making this nucleic acid stain useful for multicolor fluorescence microscopy and flow cytometry. 7-AAD appears to be generally excluded from live cells, but can be used with cells that have been fixed and permeabilized. 7-AAD has been used for cell cycle analysis by flow cytometry. 7-AAD also binds selectively to GC regions of DNA yielding a distinct banding pattern in polytene chromosomes and chromatin for use in chromosome banding studies.

View a selection guide for all nonfixable viability dyes for flow cytometry.
For Research Use Only. Not for use in diagnostic procedures.


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For Use With (Equipment): Fluorescence Microscope, Flow Cytometer
Cell Permeability: Cell-Impermeant
Sub-Cellular Localization: Nucleus, Nucleic Acids
Form: Solid
Solubility: DMSO (Dimethylsulfoxide)
Label or Dye: 7-AAD
Product Size: 1 mg
Detection Method: Fluorescent
Flow Cytometer Laser Lines: 488
Excitation⁄Emission (nm): 546⁄647
Shipping Condition: Room Temperature

Contents & storage

Contains 1 vial of 7-AAD (1 mg).

Store in freezer (-5 to -30°C) and protect from light.


Manuals & protocols

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