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Citations & References (14)
Invitrogen™
Annexin V Conjugates for Apoptosis Detection
Detect early stages of apoptosis with Annexin V stand-alone Alexa Fluor, APC, Pacific Blue, PE, FITC, and biotin conjugates using flow cytometry.
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| Catalog Number | Excitation/Emission | Flow Cytometer Laser Lines | Conjugate |
|---|---|---|---|
| A13204 | Biotin-X | ||
| A13201 | 495/519 | 488 | Alexa Fluor 488 |
| A13199 | 494/518 | 488 | FITC |
| A13202 | 578/603 | 532, 561 | Alexa Fluor 568 |
| A13203 | 590/617 | 532 | Alexa Fluor 594 |
| A23202 | 346/442 | UV | Alexa Fluor 350 |
| A23204 | 650/665 | 633-637 | Alexa Fluor 647 |
| A35108 | 555/565 | 532, 561 | Alexa Fluor 555 |
| A35109 | 679/702 | 633-637 | Alexa Fluor 680 |
| A35110 | 650/660 | 633-637 | APC (Allophycocyanin) |
| A35111 | 565/578 | 488, 532, 561 | PE |
| A35122 | 410/455 | 405 | Pacific Blue |
Catalog number A13204
Price (EUR)
694,00
Each
Conjugate:
Biotin-X
Price (EUR)
694,00
Each
Achieve quick and reliable detection of early cell apoptosis with Annexin V stand-alone conjugates for apoptosis detection. Annexin V conjugates offer up to 100-fold difference in fluorescence signal intensity between apoptotic and non-apoptotic cells using flow cytometry.
Annexin V has a high affinity for phosphatidylserine (PS), which becomes exposed on the outer leaflet of cells undergoing apoptosis. Because of this affinity, fluorescently labeled annexin V reagents are commonly used in apoptosis research.
Annexin V conjugates provide quick and reliable detection methods for studying the externalization of phosphatidylserine, an indicator of intermediate stages of apoptosis. The difference in fluorescence intensity between apoptotic and nonapoptotic cells stained with our fluorescent annexin V conjugates, as measured by flow cytometry, is typically about 100-fold.
In collaboration with Nexins Research BV, we provide the best and brightest annexin V conjugates available, including Alexa Fluor 350, 488, 555, 568, 594, 647, and 680 annexin V conjugates, as well as Annexin V APC, Biotin-X, FITC, Pacific Blue, and PE conjugates. Highly fluorescent annexin V conjugates provide quick and reliable detection methods for studying the externalization of phosphatidylserine, one of the earliest indicators of apoptosis.
The Annexin V Pacific Blue conjugate is violet excitable, making it ideal for instruments with a violet laser and for multicolor experiments that include green- or red-fluorescent dyes.
The benefits of our annexin V conjugates include:
• Conjugated to Invitrogen Alexa Fluor and eFluor dyes for brighter signals
• Conjugates for all available lasers
• Available as stand-alone reagents or easy-to-use kits
Annexin V staining to detect apoptotic cells can only be done on live cells and tissue. If samples are to be fixed post-staining, there are specific conditions required to achieve transient retention of signal. These include use of an alcohol-free, aldehyde-based fixation method, use of buffers containing Ca2+ and avoidance of surfactants/detergents. For your convenience, we also offer a concentrated annexin-binding buffer that facilitates the binding of annexin V to phosphatidylserine in apoptosis assays.
Annexin V conjugates provide quick and reliable detection methods for studying the externalization of phosphatidylserine, an indicator of intermediate stages of apoptosis. The difference in fluorescence intensity between apoptotic and nonapoptotic cells stained with our fluorescent annexin V conjugates, as measured by flow cytometry, is typically about 100-fold.
In collaboration with Nexins Research BV, we provide the best and brightest annexin V conjugates available, including Alexa Fluor 350, 488, 555, 568, 594, 647, and 680 annexin V conjugates, as well as Annexin V APC, Biotin-X, FITC, Pacific Blue, and PE conjugates. Highly fluorescent annexin V conjugates provide quick and reliable detection methods for studying the externalization of phosphatidylserine, one of the earliest indicators of apoptosis.
The Annexin V Pacific Blue conjugate is violet excitable, making it ideal for instruments with a violet laser and for multicolor experiments that include green- or red-fluorescent dyes.
The benefits of our annexin V conjugates include:
• Conjugated to Invitrogen Alexa Fluor and eFluor dyes for brighter signals
• Conjugates for all available lasers
• Available as stand-alone reagents or easy-to-use kits
Annexin V staining to detect apoptotic cells can only be done on live cells and tissue. If samples are to be fixed post-staining, there are specific conditions required to achieve transient retention of signal. These include use of an alcohol-free, aldehyde-based fixation method, use of buffers containing Ca2+ and avoidance of surfactants/detergents. For your convenience, we also offer a concentrated annexin-binding buffer that facilitates the binding of annexin V to phosphatidylserine in apoptosis assays.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
DescriptionAnnexin V, biotin-X conjugate
For Use With (Equipment)Flow Cytometer
Kit ContentsContains 1 vial of annexin V, biotin-x conjugate.
No. of Reactions100
Product TypeAnnexin V conjugate
Quantity500 μL
Shipping ConditionWet Ice
ConjugateBiotin-X
Unit SizeEach
Contents & Storage
Store in refrigerator (2–8°C).
Frequently asked questions (FAQs)
I want to study apoptosis using an Annexin V conjugate, but with adherent cells via microscopy instead of flow cytometry. Can this be done?
I am trying to label adherent cells with annexin V and am finding that everything is getting labeled. How can I fix this?
What are the advantages of flow cytometry?
What kinds of applications can I run on a flow cytometer?
Citations & References (14)
Citations & References
Abstract
Regulation of the Fas death pathway by FLICE-inhibitory protein in primary human B cells.
Journal:J Immunol
PubMed ID:10975811
'The Fas/Fas ligand (L) system plays an important role in the maintenance of peripheral B cell tolerance and the prevention of misguided T cell help. CD40-derived signals are required to induce Fas expression on virgin B cells and to promote their susceptibility to Fas-mediated apoptosis. In the current study, we
Detection of apoptotic cells by annexin V labeling at electron microscopy.
Journal:Eur J Histochem
PubMed ID:9359032
'Annexin V is a Ca(++)-binding protein which is widely used as a marker for apoptotic cells, as it binds to the phosphatidylserine residues exposed at the surface of apoptotic cells. In this paper, we describe a method for the immunogold-labeling of biotin-conjugated Annexin V, to detect apoptotic thymocytes at electron
Spatiotemporal distribution of dying neurons during early mouse development.
Journal:Eur J Neurosci
PubMed ID:10051772
'Apoptosis is a critical cellular event during several stages of neuronal development. Recently, we have shown that biotinylated annexin V detects apoptosis in vivo in various cell lineages of a wide range of species by binding to phosphatidylserines that are exposed at the outer leaflet of the plasma membrane. In
In situ detection of apoptosis during embryogenesis with annexin V: from whole mount to ultrastructure.
Journal:Cytometry
PubMed ID:9415414
'Apoptosis is of paramount importance during embryonic development. This insight stems from early studies which correlated cell death to normal developmental processes and now has been confirmed by linking aberrant cell death patterns to aberrant development. Linking apoptosis to the phenotype of a developing organism requires spatial information on the
Complement-independent, peroxide-induced antibody lysis of platelets in HIV-1-related immune thrombocytopenia.
Journal:Cell
PubMed ID:11551503
'Immunologic thrombocytopenia is seen commonly in HIV-1 infection. The pathogenesis of this problem has been unclear, but it is associated with circulating immune complexes that contain platelet membrane components and anti-platelet membrane GPIIIa49-66 IgG antibodies. These antibodies cause acute thrombocytopenia when injected into mice. We now show that purified anti-GPIIIa49-66