Extremely sensitive detection
PrestoBlue reagent enables analysis of primary cells
Excellent data obtained in just 10 minutes
Achieve assay results in a fraction of the time.
CHO-K1 cells were plated in cell culture medium in a 384-well plate in quadruplicate starting at 10,000 cells/well with a two-fold serial dilution. Cells were incubated with PrestoBlue® reagent, alamarBlue® reagent, or CellTiter-Blue® reagent for 10 minutes or 4 hours. Fluorescence measurements obtained with PrestoBlue® reagent in 10 minutes take 4 hours with alamarBlue® and CellTiter-Blue® reagents. <br><br><b>PrestoBlue® Reagent Viability Assays Are Significantly Faster Than Rezasurin-based Viability Assays, Without Any Loss of Sensitivity</b><br> PrestoBlue® reagent only requires a 10-minute incubation period, whereas alamarBlue® and CellTiter-Blue® recommend a 1 to 4 hours incubation. Top panel demonstrates the difference in fluorescence signal obtained from PrestoBlue® reagent and CellTiter-Blue® reagent following a 10-minute incubation. The dynamic range is significantly greater at the early time points for PrestoBlue® reagent.
Absorbance and fluorescence emission spectra of PrestoBlue reagent
|Compatible Cells:||Bacteria, Eukaryotic Cells, Fungal Cells|
|High-throughput Compatibility:||High-throughput Compatible|
|Incubation Time:||10 min. - 2 hrs|
|Sensitivity:||10 cells per well (384-well plate)|
|Shipping Condition:||Approved for shipment at Room Temperature or on Wet Ice|
|Detection Method:||Colorimetric, Fluorescent|
|For Use With (Equipment):||Microplate Reader, Spectrophotometer, HTS Reader|
|Label or Dye:||Resazurin|
|Format:||96-well plate, Cuvettes, 384-well plate|