Get high quality cell viability results and instant time savings with PrestoBlue® Cell Viability Reagent. PrestoBlue® is a ready to use cell permeable resazurin-based solution that functions as a cell viability indicator by using the reducing power of living cells to quantitatively measure the proliferation of cells. When added to cells, the PrestoBlue® reagent is modified by the reducing environment of the viable cell and turns red in color, becoming highly fluorescent. This color change can be detected using fluorescence or absorbance measurements.
The PrestoBlue® Cell Viability Reagent allows you to:
• Save time with an incubation step as short as 10 minutes. • Obtain high quality results with a large dynamic range • Experience convenience with the add and read homogenous format • Monitor living cells and perform downstream functional assays
10-minute incubations The PrestoBlue® Cell Viability Reagent is the only live-cell viability reagent on the market able to provide robust data typically in as little as 10 minutes following reagent addition. All other resazurin-based cell viability reagents on the market require a one to four hour incubation with the reagent prior to obtaining data.
High quality results Based on the cell type and incubation time, PrestoBlue® Cell Viability Reagent can detect as few as 12 cells per well in a 384 well plate. After ten minutes of incubation the PrestoBlue® Reagent can linearly detect cells down to 98 cells⁄well with a Z’ value of greater than 0.5.
Homogenous assay format The PrestoBlue® Cell Viability Reagent is an addition-only reagent. Without the cell lysis or solubilization steps required by other assays on the market, hands-on time is minimized.
Live cell assays The PrestoBlue® Cell Viability Reagent does not require cell lysis. Cells may be incubated for up to 24 hours in the presence of PrestoBlue® Cell Viability Product. Simply remove the reagent from the cells and replace it with growth medium for further proliferation.
For Research Use Only. Not for use in diagnostic procedures.
Bacteria, Eukaryotic Cells, Fungal Cells
96-well plate, Cuvettes, 384-well plate
10 min. - 2 hrs
Approved for shipment at Room Temperature or on Wet Ice
For Use With (Equipment)
Contents & Storage
4°C protected from light
PrestoBlue reagent enables analysis of primary cells
Extremely sensitive detection
CHO-K1 cells were plated in cell culture medium in a 384-well plate in quadruplicate starting at 10,000 cells/well with a two-fold serial dilution. Cells were incubated with PrestoBlue® reagent, alamarBlue® reagent, or CellTiter-Blue® reagent for 10 minutes or 4 hours. Fluorescence measurements obtained with PrestoBlue® reagent in 10 minutes take 4 hours with alamarBlue® and CellTiter-Blue® reagents. <br><br><b>PrestoBlue® Reagent Viability Assays Are Significantly Faster Than Rezasurin-based Viability Assays, Without Any Loss of Sensitivity</b><br> PrestoBlue® reagent only requires a 10-minute incubation period, whereas alamarBlue® and CellTiter-Blue® recommend a 1 to 4 hours incubation. Top panel demonstrates the difference in fluorescence signal obtained from PrestoBlue® reagent and CellTiter-Blue® reagent following a 10-minute incubation. The dynamic range is significantly greater at the early time points for PrestoBlue® reagent.
Excellent data obtained in just 10 minutes
Absorbance and fluorescence emission spectra of PrestoBlue reagent