CD EfficientFeed™ C AGT™ Nutrient Supplement

Catalog number:  A1327504

 Related applications:

Antibody & Cell Culture Production

| Mammalian Cell Culture

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GIBCO® CD EfficientFeed™ C AGT™ nutrient supplement is a dry format single part supplement for fed batch addition to the culture of multiple cell lines. This product is designed to work with GS CHO cell lines as a single feed without the need for additional GS supplements. CD EfficientFeed™ C AGT™ nutritient supplement is an animal origin-free, chemically defined formulation that contains no proteins, hydrolysates, or components of incompletely defined composition.

The AGT™ format is designed for large scale manufacturing and ease of use.
• Up to 2.5 – 6 Fold increases versus batch culture – higher concentration of key components
• Higher productivity in GS CHO and other selected cell lines
• Avoid issues associated with the use of hydrolysates and other alternatives while gaining superior performance
• Simplify scale-up by leveraging the value of AGT™ simple reconstitution
• Ready for larger scale use with convenient packaging options (1 L, 10 L, and 100 L)
• No components of animal origin, minimizing the opportunity for introduction of adventitious agents and facilitating the regulatory process

Which EfficientFeed™ Nutrient Supplement to Choose?
Consider CD EfficientFeed™ C AGT™ supplement when:
• You want higher productivity in GS CHO and other selected cell lines
• You are currently using CD FortiCHO™ as your base cell growth medium
• You are using a base medium with greater than 6 g/L glucose or a hydrolysate at day 0

Consider CHO CD EfficientFeed™ A supplement when:
• You are currently using CD OptiCHO™ as your base cell growth medium

Consider CHO CD EfficientFeed™ B supplement when:
• You are currently using CD CHO as your base cell growth medium

Consider the CHO CD Efficient Feed™ Kit when:
• You are using a base medium with less than 6 g/L glucose and no hydrolysate at day 0

Increased Titers
EfficientFeed™ supplements have been shown to provide fold increases in titers versus batch culture, and are an excellent starting point for process development. Furthermore, it does not require addition of a GS Supplement when used in CHO cell lines expressing endogenous glutamine synthetase.

Simple Scale-Up and Cost Savings
The ability to scale up in a dry format provides ease of handling and storage in manufacturing and other bioprocess settings. With AGT™ (Advanced Granulation Technology™) dry format, it is possible to store and use the product even more easily and safely than traditional dry format products. CD EfficientFeed™ C AGT™ supplement is offered in 1 L, 10 L and 100 L packaging that allows scale-up as a seamless process, as well as allow for cost savings when purchasing production level volumes.

AGT™ Format Ease of Use
CD EfficientFeed™ C AGT™ Nutrient Supplement delivers dry format scale-up convenience as single part AGT dry format. For more details, consult the supporting documents provided below.

For research or further manufacturing use. Not for use in diagnostic procedures.
For Research Use or Further Manufacturing. Not for use in diagnostic procedures.


Cell Line: CHO
Classification: Animal Origin-Free, Chemically Defined, Protein-Free, Serum-Free
Comments: Chemically Defined Nutrient Feeding Supplement
Culture Environment: CO2
Culture Type: Suspension Cell Culture
Endotoxin Level: Low
Form: Powder (AGT)
Format: Securitainer Capped Vial
Glutamine: No Glutamine
Included Antibiotics: No antibiotics
Inorganic Salts: Calcium, Magnesium, Sodium Phosphate
Other Additives: No Insulin
Phenol Red Indicator: No Phenol Red
Product Size: 1 L
Purity or Quality Grade: Cell Culture Grade, Qualified for Further Manufacturing, Research Grade
Serum Level: Serum-Free
Sodium Bicarbonate Buffer: Sodium Bicarbonate
Sodium Pyruvate Additive: No Sodium Pyruvate
Sterility: Nonsterile
Tests Performed: Solubility
Shipping Condition: Wet Ice

Contents & storage

Store in the dark at +2 to +8°C. Stable for 12 months when properly stored.


Manuals & protocols