GeneArt® pYES1L Vector with Sapphire™ Technology

Catalog number: A13287

Invitrogen™  Related applications: PCR Cloning | Seamless Cloning & Genetic Assembly

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Description

GeneArt® pYES1L Vector with Sapphire Technology™ is a ready-to-use, 9.3 Kb linearized BAC⁄YAC shuttle vector, used for the assembly of DNA fragments with the GeneArt® High-Order Genetic Assembly System (cat#A13285 & A13286). The vector has the capacity to clone up to 110 Kb of DNA fragments. This product only includes the linearized GeneArt® pYES1L Vector with Sapphire Technology™.

Some of the key features and elements of the GeneArt® pYES1L Vector with Sapphire Technology™ include:
TRP1 to allow selection of yeast transformants in tryptophan-deficient medium
• ARS⁄CEN origin to allow stable maintenance of the construct in yeast
• F’ for conjugation
• oriT for maintenance of large constructs in E.coli
• Spectinomycin resistance for selection in E.coli
• 6 Convenient and validated restriction sites flanking the cloning region
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Antibiotic Resistance (Bacterial): Spectinomycin
Cloning Method: Seamless Cloning
Maximum Size: 110 kb total (vector plus all inserts)
Number of Fragments: Up to 10 Fragments
Product Size: 10 reactions
Replication Origin: ARS⁄CEN, oriT
Selection Marker (Eukaryotic): TRP1
Structure: Linear
Vector Type: Seamless Cloning Vectors
Shipping Condition: Dry Ice

Contents & storage

The product contains 5 vials of linear GeneArt® pYES1L Vector with Sapphire Technology™ at a concentration of 50 ng⁄ml. Each vial contains enough plasmid for 2 cloning reactions using the GeneArt® High-Order Genetic Assembly System (needs to be purchased separately).

Store the product at -80°C.

The vector is guaranteed stable for 6 months when properly stored.

Documents

Manuals & protocols