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Invitrogen™
GeneArt™ pYES1L Vector with Sapphire™ Technology
Catalog number: A13287
Invitrogen™
GeneArt™ pYES1L Vector with Sapphire™ Technology
Catalog number: A13287
Catalog Number
A13287
Unit Size
10 reactions
Price (USD)
Price: 397.00
Your Price:
Availability
-
Quantity
| Catalog Number | Unit Size | Price (USD) | Availability | Quantity | |
|---|---|---|---|---|---|
| A13287 | 10 reactions | Price: 397.00 Your Price: | - |
Product Overview
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GeneArt™ pYES1L Vector with Sapphire Technology™ is a ready-to-use, 9.3 Kb linearized BAC⁄YAC shuttle vector, used for the assembly of DNA fragments with the GeneArt™ High-Order Genetic Assembly System (cat#A13285 & A13286). The vector has the capacity to clone up to 110 Kb of DNA fragments. This product only includes the linearized GeneArt™ pYES1L Vector with Sapphire Technology™.
Some of the key features and elements of the GeneArt™ pYES1L Vector with Sapphire Technology™ include:
• TRP1 to allow selection of yeast transformants in tryptophan-deficient medium
• ARS⁄CEN origin to allow stable maintenance of the construct in yeast
• F’ for conjugation
• oriT for maintenance of large constructs in E.coli
• Spectinomycin resistance for selection in E.coli
• 6 Convenient and validated restriction sites flanking the cloning region
Some of the key features and elements of the GeneArt™ pYES1L Vector with Sapphire Technology™ include:
• TRP1 to allow selection of yeast transformants in tryptophan-deficient medium
• ARS⁄CEN origin to allow stable maintenance of the construct in yeast
• F’ for conjugation
• oriT for maintenance of large constructs in E.coli
• Spectinomycin resistance for selection in E.coli
• 6 Convenient and validated restriction sites flanking the cloning region
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Antibiotic Resistance Bacterial
Spectinomycin
Cloning Method
Seamless Cloning
Sample Type
DNA
For Use With (Application)
Cloning
No. of Reactions
10 Reactions
Number of Fragments
Up to 10 Fragments
Product Line
GeneArt™
Replication Origin
ARS⁄CEN, oriT
Selection Marker Eukaryotic
TRP1
Shipping Condition
Dry Ice
Size
110 kb total (vector plus all inserts)
Vector
pYES1L Linearized
Contents & Storage
The product contains 3 vials of linear GeneArt pYES1L Vector with Sapphire Technology at a concentration of 50 ng/mL. Vials contain enough plasmid for 10 cloning reactions using the GeneArt High-Order Genetic Assembly System (needs to be purchased separately).
Store the product at -80°C.
The vector is guaranteed stable for 6 months when properly stored.
Store the product at -80°C.
The vector is guaranteed stable for 6 months when properly stored.
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