Invitrogen™

CSM Media for Mav203 Yeast Cells

Catalog number: A13292
Invitrogen™

CSM Media for Mav203 Yeast Cells

Catalog number: A13292
Catalog Number
A13292
Unit Size
2 L
Price (USD)
294.00
Your Price:
Availability
***
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
A132922 L
294.00
Your Price:
***
Product Overview
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The CSM Media for Mav203 Yeast Cells is a specifically designed media for making agar plates that enable the growth of Saccharomyces cerevisae MaV203 cells. This product comes with enough reagents to prepare 2 liters of solid media with the following components per liter:

• CSM (without tryptophan): 0.74 g
• Yeast Nitrogen Base: 6.66 g
• Ammonium Sulfate: 5 g
• Glucose: 20 g
• Agar: 20 g

Compatible with All MaV203 Applications
The CSM Media is optimized for the GeneArt™ High-Order Genetic Assembly System, including the recovery for transformations of vectors once they have been adapted to yeast using the GeneArt™ High-Order Vector Conversion Cassette. The media can also be used to grow MaV203 competent yeast cells, both library and sub-cloning scale (cat# 11281-011 and 11445-012).

Plate Preparation Protocol
For each liter, use one pouch of CSM -Trp Agar Yeast Media minus Glucose and 1 bottle of 20% Glucose. Dissolve the contents of the pouch in 900ml of distilled water and autoclave for 20 minutes on liquid cycle. Allow the solution to cool to 55°C and then add one bottle of 20% Glucose (100ml). Mix well and pour plates.

Specifications

Product Type
CSM Media
Sterility
Non-sterile
Bacterial or Yeast Strain
MaV203
Final Product Type
Agar Plates
For Use With
Optimized for use with the GENEART™ High-Order Genetic Assembly System, A13285
Preparation Method
Autoclave
Shipping Condition
Room Temperature
Target Organism Class
S. cerevisiae
Product Line
GeneArt™
Form
Liquid, Powder
Media Type
CSM Media

Contents & Storage

CSM Media for MaV203 Yeast Cells has enough reagents to prepare 2 liters of agar media. The product contains two pouches of premixed powder and two 100 ml bottles of filter-sterilized, 20% glucose.

Store all components at room temperature.

All components are guaranteed stable for 6 months when properly stored.

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