Fluoresceinyl Glycine Amide (5-(Aminoacetamido)Fluorescein)

Catalog number: A1363

Molecular Probes™  Related applications: Labeling Chemistry | Protein Labeling & Crosslinking

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Description

The primary aliphatic amine of 5-(aminoacetamido)fluorescein (fluoresceinyl glycine amide) can be reversibly coupled to aldehydes and ketones to form a Schiff base - which can be reduced to a stable amine derivative by sodium borohydride (NaBH4) or sodium cyanoborohydride (NaCNH3) to form new probes. Carboxylic acids of proteins and other water-soluble biopolymers can be coupled to this molecule in aqueous solution using water-soluble carbodiimides such as EDAC (E2247). The glycine may be the better amine probe for direct carbodiimide-mediated coupling, since it is likely to remain unprotonated at a lower pH than other aliphatic amines.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Label or Dye: FITC (Fluorescein)
Chemical Reactivity: Carboxylic Acid, Aldehyde, Ketone
Product Size: 10 mg
Shipping Condition: Room Temperature

Contents & storage

Store at room temperature and protect from light.

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