CTS™ KnockOut™ DMEM/F-12 This product, and/or its packaging, incorporates one or more environmental/green features. Click on this icon to learn more.

Catalog number:  A1370801

 Related applications:

Clinical & Translational Research

| Stem Cell Culture

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Cell Therapy Systems KnockOut™ DMEM/F-12 CTS™ is a serum free, low osmolality medium without L-glutamine or HEPES buffer that is optimized for the growth of human embryonic stem cells (ESC) and induced pluripotent stem cells (iPSC). KnockOut™ DMEM/F-12 CTS™ media is designed for use with KnockOut™ SR and KnockOut™ SR XenoFree CTS™ supplements.

Designed for reliable and consistent performance

• Perform ESC/iPSC culturing with little to no spontaneous differentiation
• Be ready for clinical research with this cGmP manufactured formula and support documentation

Consistent Results Without Fetal Bovine Serum
Cell Therapy Systems KnockOut™ DMEM/F-12 CTS™ media is part of the KnockOut™ line of media products that have delivered reliable results for ESC and now iPSC culturing for years. This fetal bovine free media leads to little or no spontaneous differentiation, and frees you from repeated screens.

Equivalent Morphology
Stem cells cultured in KnockOut™ DMEM/F-12 CTS™ media show normal morphology and karyotype when compared to control stem cells. You can also expect equivalent morphology to manually passaged hESCs, even after enzymatic passaging.

Simplify Your Transition into Clinical Research
The Gibco® Cell Therapy Systems (CTS™) brand offers a broad array of products for use in cell therapy applications, including media, reagents, growth factors, enzymes, cell selection/expansion reagents, and devices. Gibco® Cell Therapy Systems (CTS™) products deliver:
• Reduced burden in qualifying reagents
• Included appropriate certificates of analyses and origin
• Convenient access to our Drug Master File (DMF)

For Research Use or Non-Commercial Manufacturing of Cell-Based Products for Clinical Research.

CAUTION: Not intended for direct administration to humans or animals
For Research Use Only. Not for use in diagnostic procedures.


Manufacturing Quality: cGMP for medical devices, 21 CFR Part 820 and ISO 13485
Glutamine: No Glutamine
Phenol Red Indicator: Phenol Red
Form: Liquid
Sodium Pyruvate Additive: Sodium Pyruvate
Serum Supplementation: KnockOut™
Classification: Animal Origin-Free
Culture Environment: CO2
Culture Type: Adherent Cell Culture
Product Size: 500 mL
Quantity: 1 bottle(s)
Sterility: Sterile
Supplements: Requires GlutaMax supplement
Volume (Metric): 500 ml
Green Features: Sustainable packaging
Shipping Condition: Room Temperature

Contents & storage

Store at 2 to 8°C and protect from light.


Manuals & protocols