CTS™ Synth-a-Freeze™ Medium

Catalog number:  A1371301

 Related applications:

Cell Counting, Viability, & Cryopreservation

Error loading your content!

  Catalog number
Select a plan
Unit size
Price ({{currency}}) Availability Qty
{{product.sku}} {{product.sku}} also known as {{product.formattedSku}} 
Pro add-ons

Your on-site stock

›› {{supplyCenter.scName}}({{scProduct.stockOnHand}} In stock)
›› {{supplyCenter.scName}}(Out of stock)
›› {{supplyCenter.scName}}
This item is not currently available on-site. Depending on your Supply Center settings you may be able to add the item to cart above else use the Order Non-Stocked Items' tab on the Supply Center home page.
Back to top


CTS™ (Cell Therapy Systems) Synth-a-Freeze® medium is a chemically defined, protein-free liquid cryopreservation medium intended for freezing and storing a variety of mammalian cell types including stem cells. Synth-a-Freeze® CTS™ media is optimally formulated without antibiotics, antimycotics, hormones, growth factors, serum, or protein and buffered with HEPES and sodium bicarbonate.

Use for cryopreserving a variety of primary and stem cells

• Save time and effort with 4 degree stable storage of thawed media and compatibility with a variety freezing protocols
• Be ready for clinical research with this cGmP manufactured, chemically defined, protein-free formula

Cryopreserve a Variety of Cell Types
Cell Therapy Systems Synth-a-Freeze® CTS™ media performs comparable to our standard, serum-containing cryopreservation medium for cryopreserving a variety of cell types including human Embryonic Stem Cells, Neural Stem Cells (Ref 1), Mesenchymal Stem Cells and Keratinocytes (Ref 2). We do not recommend using Synth-a-Freeze® media for cryopreservation of human epidermal melanocytes.

Simple Incorporation into Your Current Freezing Protocol
Synth-a-Freeze® CTS™ media can be used with any standard freezing protocol. Just add thawed Cell Therapy Systems Synth-a-Freeze® CTS™ media to detached and pelleted cells, aliquot, and freeze. For optimal results, the use of a controlled-rate freezer is recommended.

Simplify Your Transition into Clinical Research
The Gibco® Cell Therapy Systems (CTS™) brand offers a broad array of products for use in cell therapy applications, including media, reagents, growth factors, enzymes, cell selection/expansion reagents, and devices. Gibco® Cell Therapy Systems (CTS™) products deliver:
• Reduced burden in qualifying reagents
• Included appropriate certificates of analyses and origin
• Convenient access to our Drug Master File (DMF)

For Research Use or Manufacturing of Cell, Gene, or Tissue-Based Products.

CAUTION: Not intended for direct administration into humans or animals

• S. Shin and M. Vemuri. Culture and Differentiation of Human Neural Stem Cells. L.C. Doering (ed). Protocols for Neural Cell Culture. Springer Protocol Handbooks.
• S. Lemaire, K. Kosowska-Shick, P. Appelbaum, G. Verween, P. Tulkens, and F. Van Bambeke. Cellular Pharmacodynamics of the Novel Biaryloxazolidinone Radezolid: Studies with Infected Phagocytic and Nonphagocytic cells, Using Staphylococcus aureus, Staphylococcus epidermidis, Listeria monocytogenes, and Legionella pneumophila. Antimicrob Agents Chemother 54(6) 2010: 2549-2559.

For Research Use Only. Not for use in diagnostic procedures.


Serum Level: Serum-Free
Classification: Animal Origin-Free
Endotoxin Level: < 1 EU/ml
Form: Liquid
Other Additives: DMSO (10%)
Phenol Red Indicator: Phenol Red
Product Size: 50 mL
Sterility: Sterile
Volume (Metric): 50 ml
Shipping Condition: Dry Ice

Contents & storage

Store at -5 to -20°C


Manuals & protocols