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Citations & References (6)
Gibco™
CTS™ Synth-a-Freeze™ Medium
CTS™ (Cell Therapy Systems) Synth-a-Freeze™ medium is a chemically defined, protein-free liquid cryopreservation medium intended for freezing and storing aRead more
| Catalog Number | Quantity |
|---|---|
| A1371301 | 50 mL |
Catalog number A1371301
Price (USD)
340,50
Each
Quantity:
50 mL
Price (USD)
340,50
Each
CTS™ (Cell Therapy Systems) Synth-a-Freeze™ medium is a chemically defined, protein-free liquid cryopreservation medium intended for freezing and storing a variety of mammalian cell types including stem cells. Synth-a-Freeze™ CTS™ media is optimally formulated without antibiotics, antimycotics, hormones, growth factors, serum, or protein and buffered with HEPES and sodium bicarbonate.
• Use for cryopreserving a variety of primary and stem cells
• Save time and effort with 4 degree stable storage of thawed media and compatibility with a variety freezing protocols
• Be ready for clinical research with this cGmP manufactured, chemically defined, protein-free formula
Cryopreserve a Variety of Cell Types
Cell Therapy Systems Synth-a-Freeze™ CTS™ media performs comparable to our standard, serum-containing cryopreservation medium for cryopreserving a variety of cell types including human Embryonic Stem Cells, Neural Stem Cells (Ref 1), Mesenchymal Stem Cells and Keratinocytes (Ref 2). We do not recommend using Synth-a-Freeze™ media for cryopreservation of human epidermal melanocytes.
Simple Incorporation into Your Current Freezing Protocol
Synth-a-Freeze™ CTS™ media can be used with any standard freezing protocol. Just add thawed Cell Therapy Systems Synth-a-Freeze™ CTS™ media to detached and pelleted cells, aliquot, and freeze. For optimal results, the use of a controlled-rate freezer is recommended.
Simplify Your Transition into Clinical Research
The Gibco™ Cell Therapy Systems (CTS™) brand offers a broad array of products for use in cell therapy applications, including media, reagents, growth factors, enzymes, cell selection/expansion reagents, and devices. Gibco™ Cell Therapy Systems (CTS™) products deliver:
• Reduced burden in qualifying reagents
• Included appropriate certificates of analyses and origin
• Convenient access to our Drug Master File (DMF)
For Research Use or Manufacturing of Cell, Gene, or Tissue-Based Products.
CAUTION: Not intended for direct administration into humans or animals
References:
• S. Shin and M. Vemuri. Culture and Differentiation of Human Neural Stem Cells. L.C. Doering (ed). Protocols for Neural Cell Culture. Springer Protocol Handbooks.
• S. Lemaire, K. Kosowska-Shick, P. Appelbaum, G. Verween, P. Tulkens, and F. Van Bambeke. Cellular Pharmacodynamics of the Novel Biaryloxazolidinone Radezolid: Studies with Infected Phagocytic and Nonphagocytic cells, Using Staphylococcus aureus, Staphylococcus epidermidis, Listeria monocytogenes, and Legionella pneumophila. Antimicrob Agents Chemother 54(6) 2010: 2549-2559.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Cell TypeVarious (including types of human Embryonic Stem Cells, Neural Stem Cells, Mesenchymal Stem Cells, and Keratinocytes)
For Use With (Application)Cell and Gene Therapy Research, Development, and Manufacturing
FormatBottle
Manufacturing QualityISO 13485, MDSAP, FDA-registered, 21 CFR 820
Product TypeCryopreservation Medium
Quantity50 mL
Shelf Life18 months from date of manufacture
Shipping ConditionDry Ice
ClassificationAnimal Origin-free
FormLiquid
SterilitySterile-filtered
With AdditivesDMSO (10%)
Unit SizeEach
Contents & Storage
Store at –20°C.
Frequently asked questions (FAQs)
What is CTS?
If the Synth-a-Freeze Cryopreservation Medium is not suitable for melanocytes, how should I freeze melanocytes?
Is there a Drug Master File for the Synth-a-Freeze Cryopreservation Medium?
What can I use Synth-a-Freeze Cryopreservation Medium for?
Was the tissue cryopreserved prior to isolation of primary cells?
Citations & References (6)
Citations & References
Abstract
Serum-free suspension culturing of human cells: adaptation, growth, and cryopreservation.
Journal:Bioprocess Biosyst Eng
PubMed ID:25822314
Human cell lines have attracted great interest because they are capable of producing glycosylated proteins that are more similar to native human proteins, thereby reducing the potential for immune responses. However, these cells have not been extensively characterized and cultured under serum-free suspension conditions. In this work, we describe the
Amnion epithelial cell isolation and characterization for clinical use.
Journal:Curr Protoc Stem Cell Biol
PubMed ID:20373516
Human amnion epithelial cells (hAECs) are a heterologous population positive for stem cell markers; they display multilineage differentiation potential, differentiating into cells of the endoderm (liver, lung epithelium), mesoderm (bone, fat), and ectoderm (neural cells). They have a low immunogenic profile and possess potent immunosuppressive properties. Hence, hAECs may be
Cellular pharmacodynamics of the novel biaryloxazolidinone radezolid: studies with infected phagocytic and nonphagocytic cells, using Staphylococcus aureus, Staphylococcus epidermidis, Listeria monocytogenes, and Legionella pneumophila.
Journal:Antimicrob Agents Chemother
PubMed ID:20385852
Radezolid is a novel biaryloxazolidinone in clinical development which shows improved activity, including against linezolid-resistant strains. In a companion paper (29), we showed that radezolid accumulates about 11-fold in phagocytic cells, with approximately 60% of the drug localized in the cytosol and approximately 40% in the lysosomes of the cells.
Rapid and Efficient Generation of Transgene-Free iPSC from a Small Volume of Cryopreserved Blood.
Journal:
PubMed ID:25951995
Human peripheral blood and umbilical cord blood represent attractive sources of cells for reprogramming to induced pluripotent stem cells (iPSCs). However, to date, most of the blood-derived iPSCs were generated using either integrating methods or starting from T-lymphocytes that have genomic rearrangements thus bearing uncertain consequences when using iPSC-derived lineages
Biological impact of xeno-free chemically defined cryopreservation medium on amniotic epithelial cells.
Journal:Stem Cell Res Ther
PubMed ID:26758986
'Amnion-derived stem cells have been proposed for cell replacement therapy and tissue regeneration. An easily accessible cell source, the placenta, allows us to potentially establish a bio-bank of cells for immunotype matched clinical applications. Several xeno-free (XF) cryopreservation media are currently available for pluripotent stem cells, however, these media have