The Freedom™ DG44™ Kit can be used as a replacement for the discontinued OptiCHO™ Protein Express Kit (#A10999-01)
Questions on our cell line development kits? Get help from our Life Technologies Process Development Team.

Freedom™ DG44 Kit This product, and/or its packaging, incorporates one or more environmental/green features. Click on this icon to learn more.

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Gibco® Freedom® DG44 Kit is an easy-to-use, beginning-to-end product, for cloning and expression of recombinant proteins in dihydrofolate reductase deficient (DHFR-) Chinese Hamster Ovary cells. The Gibco® Freedom® DG44 Kit includes components for transfection, expression, clone creation, and stable cell line selection, all in a conveniently packaged kit. The Gibco® Freedom® DG44 Kit also delivers a proven host cell line with simplified commercial licensing options.

• Flexible commercial licensing without the need for royalties
• Proven host used in approved products and a wide array of proteins
• Complete workflow that can take your gene of interest from transfection to lead clone typically in less than 6 months
• Convenient packaging, for simplified ordering and storage

Commercial Licensing that is Simple, Flexible and Without Royalties
Multiple companies provide CHO (Chinese Hamster Ovary) stable cell line development services and products that require annual fees during development of your therapeutic protein. In addition, royalties may be required on the sales of your therapeutic protein upon commercialization. Gibco® Freedom® Kits provide you with the tools that enable you or your CRO provider to develop your stable cell line for just the price of the kit itself. Furthermore, as you move towards commercial use, a flexible license structure is available without the burden of royalties and works to keep it as simple as a onetime payment (Fig 1). For more information regarding licensing, email us at

Host Cell Background with Proven Results
DG44 cells have been a choice of scientists for decades for the expression of IgG and numerous blood proteins. Protein expression levels can vary significantly depending on the class of protein, however gram per liter levels of IgG expression has been demonstrated using these DG44 parental cells. So you can be confident that if your work calls for use of DG44 cells, they will deliver expected protein levels.

Complete, Fast, and Efficient Workflow
The Gibco® Freedom® DG44 Kit’s complete, proven, and fully integrated workflow allows you to move from transfection to lead clone typically in less then 6 months, helping to reduce hands on time and Fulltime Equivalent (FTE) requirements (Fig 2). We have implemented a single round of MTX (methotrexate) amplification in the protocol, which in our hands provided up to 120 fold titer improvement in less than 6 weeks (Fig 3). The entire workflow is performed in animal origin free (AOF) medium. The kit also offers the following advantages:

• Cloning of one or two genes that encode your protein(s) of interest
• High efficiency transfection of DNA into DG44 Cells (cGMP-banked)
• Included flash drive with loaded manual

New Convenient Packaging
To free you to focus on the creation of your stable cell line rather than on figuring out where and when all the components of your experiment are arriving, Freedom® DG44 Kit introduces the smART™ tote packaging system (shown in above product photos). All components for the creation of your expression cell line are delivered in soft cooler totes along with a parts flyer, and a flash drive containing the manual. Upon delivery you have all the information needed for correct storage and use of components consolidated in the two smART™ tote shipment coolers.

Access to Gibco® Freedom® Kit Support
If any specific support is needed beyond the manual, please contact your account manager to obtain a Process Science Manager consultation. In addition, one of our support scientists may check in with you to ensure that your experience with the kit is as successful as possible. For further assistance, please email our support team at
For Research Use Only. Not for use in diagnostic procedures.


Cell Line: CHO DG44
Delivery Method: Transfection
Expression: Mammalian
Key Function: Protein Production, Stable Cell Line Development
Product Size: 1 kit
Promoter: CMV
Quantity: 1 kit
Selection Agent (Eukaryotic): Geneticin® (G-418), MTX (Methotrexate)
Selection Marker (Eukaryotic): DHFR, NeoR
Special Elements: EMCV IRES
Green Features: Sustainable packaging

Contents & storage

CD DG44 Medium: 2°C to 8°C, protect from light
CD OptiCHO™ Medium: 2°C to 8°C, protect from light
OptiPRO™ SFM: 2°C to 8°C, protect from light
FreeStyle™ MAX Reagent: 2°C to 8°C, do not freeze
Pluronic®: F68 15°C to 30°C

DG44 (CHO) cGMP Banked suspension dhfr (dihydrofolate reductase) Negative Cells: liquid nitrogen
One Shot® TOP10 Chemically Competent E. coli: –80°C
pcDNA 3.3 TOPO® TA Vector Kit: –5°C to –20°C
pOptiVEC™ TOPO® TA Vector Kit: –5°C to –20°C
Gentamicin™: –5°C to –20°C


Manuals & protocols