M9 Minimal Salts (2X) This product, and/or its packaging, incorporates one or more environmental/green features. Click on this icon to learn more.

Catalog number:  A1374401

 Related applications:

Protein Expression

| Transformation

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Gibco® M9 Minimal Salts (2X) is a ready-to-use liquid growth media used for bacterial culture. Our filter-sterilized Gibco® M9 Minimal Salts (2X) can be used for nearly all research applications that require a liquid bacterial culturing step such as protein expression, cloning, and expansion.
• Gibco® microbiological media
• Convenient ready-to-use liquid format
• 1 L size available

M9 Minimal Salts (2X) Recipe
M9 media is a commonly used bacterial media for cultivation and maintenance of Escherichia coli (E. coli) strains. Our Gibco® M9 Minimal Salts (2X) is a concentrated base formulation that can be supplemented with various carbon sources and amino acids to make a complete M9 medium.

Formulation per One Liter:
25.6 g   Sodium phosphate (dibasic) heptahydrate
6 g   Monopotassium phosphate
1 g   Sodium chloride
2 g   Ammonium chloride

Ready-to-Use in Optimized Gibco® Media Bottles
Each bottle of Gibco® M9 Minimal Salts (2X) is filter-sterilized and ready-to-use in ergonomic Gibco® media bottles with angled openings for simplified pipetting.

For research use only. Not intended for human or animal therapeutic or diagnostic use.
For Research Use Only. Not for use in diagnostic procedures.


Final Product: Liquid Medium
Preparation Method: None (Ready-to-Use)
Form: Liquid
Target Organism Class: E. coli
Product Line: Gibco®
Product Size: 1 L
Green Features: Sustainable packaging
Shipping Condition: Room Temperature

Contents & storage

1 liter M9 Minimal Salts (2X), liquid; store at room temperature


Manuals & protocols