Gibco™

FreeStyle™ F17 Expression Medium

Catalog number: A1383503
Gibco™

FreeStyle™ F17 Expression Medium

Catalog number: A1383503
Catalog Number
A1383503
Unit Size
10 L
Price (USD)
1,338.00
Your Price:
Availability
***
Quantity
Catalog Number
A1383501
also known as A13835-01
Unit Size
1 L
Price (USD)
149.00
Your Price:
Availability
***
Quantity
Catalog Number
A1383502
also known as A13835-02
Unit Size
6 x 1 L
Price (USD)
816.00
Your Price:
Availability
***
Quantity
Catalog Number
Unit Size
20 L
Price (USD)
2,560.00
Your Price:
Availability
***
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
A138350310 L
1,338.00
Your Price:
***
A1383501
also known as A13835-01
1 L
149.00
Your Price:
***
A1383502
also known as A13835-02
6 x 1 L
816.00
Your Price:
***
A138350420 L
2,560.00
Your Price:
***
Product Overview
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Additional Information
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Recommendations
Gibco FreeStyle F17 Expression Medium is a chemically defined, protein-free, serum-free medium developed to support the growth and transfection of HEK293 cells in suspension. The addition of 4–8 mM L-glutamine or GlutaMAX supplement is required before use.

Features:
• Little or no required adaptation
• Supports suspension growth and transfection of HEK293 cells
• Chemically defined, protein-free, serum-free formulation
• Compatible with multiple transient production systems
• Scalability in spinner flasks and bioreactors

Little or no required adaptation
293-F, 293-H, and FreeStyle 293-F cells in suspension can be cultured directly into FreeStyle F17 Expression Medium without adaptation. Cells adapted to other commercially available serum-free media can be subcultured directly into FreeStyle F17 medium, usually without any further adaptation. Cells usually require adaptation from serum-containing formulations.

Growth and transfection of HEK293 cells
FreeStyle F17 Expression Medium supports 293-F cells at a peak density of 6–7 x 106 viable cells per mL. Cells cultured in FreeStyle F17 medium can be transfected using a wide variety of transfection reagents, including lipid-based transfection reagents, such as 293fectin and Freestyle MAX Reagent.

Scalability in spinner flasks and bioreactors
Production using FreeStyle F17 Expression Medium can be scaled up in spinner flasks or bioreactors. The appropriate spinner or impeller speed and seeding density should be optimized for each system. Depending on the impeller design and speed, it may be necessary to supplement FreeStyle F17 medium with additional Pluronic F-68 to avoid sheer stress in the culture.

cGMP manufacturing and quality system
FreeStyle F17 Expression Medium is manufactured at a cGMP-compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards.

Design your own medium specific to your needs
Whether it’s a bench-level experiment or large-scale biomanufacturing process, we offer specialized formats, packaging, and quality levels specific to your needs. With Gibco cGMP Media Custom Services you can put the manufacturing power and years of technical experience to work for you.

Specifications

Classification
Animal Origin-Free, Chemically Defined, Protein-Free, Serum-Free
Culture Environment
CO2
Culture Type
Suspension Cell Culture
Endotoxin Level
< 1 EU/ml
For Use With (Application)
Protein expression, viral vector production
Glutamine
No Glutamine
Product Line
Freestyle™
Purity or Quality Grade
Qualified for Further Manufacturing
Quantity
10 L
Serum Level
Serum-Free
Shipping Condition
Room Temperature
Sterility
Sterile
Format
Liquid
Product Type
F17 Expression Medium
Cell Line
HEK293
Concentration
1 X
Additive
4 to 8 mM L-glutamine or GlutaMAX™

Contents & Storage

Storage conditions: 2-8°C. Protect from light
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture

Figures

Frequently asked questions (FAQs)

Citations & References