Gibco™
Geltrex™ LDEV-Free Reduced Growth Factor Basement Membrane Matrix
Catalog number: A1413201
Related applications:Description
Geltrex™ LDEV-Free Reduced Growth Factor Basement Membrane Matrix is a soluble form of basement membrane extracted from murine Engelbreth-Holm-Swarm (EHS) tumors and is free of viruses, including lactose dehydrogenase elevating virus (LDEV), sometimes found in this type of preparation. The major components of Geltrex™ matrix include laminin, collagen IV, entactin, and heparin sulfate proteoglycans. It is ideal for the promotion and maintenance of differentiated phenotypes in a variety of cell cultures including primary epithelial cells, endothelial cells, smooth muscle cells, and human induced pluripotent stem cells (iPSC). Geltrex™ matrix offers, on average, a 2-fold reduction in several growth factors. As a result, analysis of specific growth factor effects in applications requiring basement membrane extract can be carried out in a controlled and reproducible format.Key Features of Geltrex™ LDEV-Free Reduced Growth Factor Basement Membrane Matrix:
• Free of lactose dehydrogenase elevating virus (LDEV) making it ideal for all types of cell culture and mouse in vivo applications
• Consistent protein concentration lot-to-lot helps reduce the need to screen lots
• Protocols for various applications, including tube formation assays, are available
Gain Reliability and Consistency in Your hESC Studies
Recent advances in processing EHS tumor cells have resulted in virus free basement membrane extracts. Every lot is tested by RT-PCR methods so you can now have confidence that every lot of Geltrex™ is virus free. Additionally, our consistent manufacturing process helps ensure a protein concentration with very little variation from lot-to-lot, averaging 15 mg/ml so you can have confidence in every purchase.
Investigate Angiogenesis with Validated Gibco® Protocols
There are many applications for Geltrex™ LDEV-Free reduced growth factor basement membrane matrix, each requiring different thicknesses and concentrations. In general, a protein concentration of >5 mg/ml is used for differentiation studies of primary cells. Geltrex™ LDEV-Free reduced growth factor basement membrane matrix has been used to develop tube formation assay protocols using Gibco® primary cells and specialty media. See this and other angiogenesis assay protocols
For Research Use Only. Not for use in diagnostic procedures.
Specifications
| Classification: | Reduced Growth Factors |
|---|---|
| Endotoxin Level: | Low |
| Format: | Tube(s) |
| Product Line: | Gibco™ |
| Purity or Quality Grade: | Cell Culture Grade |
| Quantity: | 1mL |
| Serum Level: | Serum-Free |
| Shipping Condition: | Dry Ice |
| Volume (Metric): | 1 ml |
| Cell Type: | Endothelial Cell, Epithelial Cell, Human Induced Pluripotent Stem Cell, Smooth Muscle Cell |
| Culture Type: | Adherent Cell Culture |
| Form: | Frozen |
| Phenol Red Indicator: | No Phenol Red |
| Species: | Mouse |
| Product Type: | Basement Membrane Matrix |
| Sterility: | Sterile |
Contents & storage
Geltrex™ LDEV-Free Reduced Growth Factor Basement Membrane Matric comes as a sterile protein solution in 1 mL vials.
Store at -20 to -80°C.
Geltrex™ Matrix is stable for 18 months from date of manufacture when properly stored.
Store at -20 to -80°C.
Geltrex™ Matrix is stable for 18 months from date of manufacture when properly stored.
