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Citations & References (14)
Gibco™
Geltrex™ LDEV-Free Reduced Growth Factor Basement Membrane Matrix
A soluble form of basement membrane extracted from murine Engelbreth-Holm-Swarm (EHS) tumors. It is free of viruses, including lactose dehydrogenase elevating virus (LDEV), sometimes found in this type of preparation.
| Catalog Number | Quantity |
|---|---|
| A1413201 | 1 mL |
NOTICE: This product will no longer be restocked — switch now to the replacement product Geltrex™ Flex Reduced Growth Factor matrix, delivering the same trusted performance in expanded vial sizes, along with a new organoid qualified matrix choice.
Catalog number A1413201
Geltrex™ LDEV-Free Reduced Growth Factor Basement Membrane Matrix is a soluble form of basement membrane extracted from murine Engelbreth-Holm-Swarm (EHS) tumors and is free of viruses, including lactose dehydrogenase elevating virus (LDEV), sometimes found in this type of preparation. The major components of Geltrex™ matrix include laminin, collagen IV, entactin, and heparin sulfate proteoglycans. It is ideal for the promotion and maintenance of differentiated phenotypes in a variety of cell cultures including primary epithelial cells, endothelial cells, smooth muscle cells, and human induced pluripotent stem cells (iPSC). Geltrex™ matrix offers, on average, a 2-fold reduction in several growth factors. As a result, analysis of specific growth factor effects in applications requiring basement membrane extract can be carried out in a controlled and reproducible format.
Key Features of Geltrex™ LDEV-Free Reduced Growth Factor Basement Membrane Matrix:
- Free of lactose dehydrogenase elevating virus (LDEV) making it ideal for all types of cell culture and mouse in vivo applications
- Consistent protein concentration lot-to-lot helps reduce the need to screen lots
- Protocols for various applications, including tube formation assays, are available
Gain Reliability and Consistency in Your hESC Studies
Recent advances in processing EHS tumor cells have resulted in virus free basement membrane extracts. Every lot is tested by RT-PCR methods so you can now have confidence that every lot of Geltrex™ is virus free. Additionally, our consistent manufacturing process helps ensure a protein concentration with very little variation from lot-to-lot, averaging 15 mg/ml so you can have confidence in every purchase.
Investigate Angiogenesis with Validated Gibco™ Protocols
There are many applications for Geltrex™ LDEV-Free reduced growth factor basement membrane matrix, each requiring different thicknesses and concentrations. In general, a protein concentration of >5 mg/ml is used for differentiation studies of primary cells. Geltrex™ LDEV-Free reduced growth factor basement membrane matrix has been used to develop tube formation assay protocols using Gibco™ primary cells and specialty media.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Cell TypeVariety of cultured cells, including primary epithelial cells, endothelial cells, smooth muscle cells, and human induced pluripotent stem cells (iPSC)
Concentration12 to 18 mg/mL
Endotoxin LevelLow
FormatTube(s)
Product LineGibco
Product TypeBasement Membrane Matrix
Purity or Quality GradeCell Culture Grade
Quantity1 mL
Shelf Life36 months
Shipping ConditionDry Ice
SpeciesMouse
ClassificationReduced Growth Factor
Culture Type3D Cell Culture, Mammalian Cell Culture, Neuronal Cell Culture, Stem Cell (Human, iPS - Induced Pluripotent Stem, Embryonic), Adherent Cell Culture
FormFrozen
Serum LevelSerum-free
SterilitySterile
Without AdditivesNo Phenol Red
Unit SizeEach
Contents & Storage
Geltrex™ LDEV-Free Reduced Growth Factor Basement Membrane Matric comes as a sterile protein solution in 1 mL vials.
Store at -20 to -80°C.
Geltrex™ Matrix is stable for 18 months from date of manufacture when properly stored.
Store at -20 to -80°C.
Geltrex™ Matrix is stable for 18 months from date of manufacture when properly stored.
Frequently asked questions (FAQs)
What growth factors are reduced in the Geltrex LDEV-Free Reduced Growth Factor Basement Membrane Matrix (Cat. No. A1413202)?
What volume do you recommend for thin coating 35 mm dishes with Geltrex LDEV-Free Reduced Growth Factor Basement Membrane Matrix (Cat. No. A1413201, A1413202) or Geltrex LDEV-Free, hESC-Qualified, Reduced Growth Factor Basement Membrane Matrix (Cat. No. A1413301, A1413302)?
Can I refreeze and reuse Geltrex LDEV-Free Reduced Growth Factor Basement Membrane Matrix after having used it in an experimental setup?
How do I characterize human embryonic stem ( ES) cells?
How are human embryonic stem (ES) cells derived?
Citations & References (14)
Citations & References
Abstract
Microgels on-demand.
Journal:
PubMed ID:24566526
'Three-dimensional (3D) hydrogel structures are finding use in fundamental studies of self-assembly, rheology, and 3D cell culture. Most techniques for 3D hydrogel formation are ''single pot'', in which gels are not addressable after formation. For many applications, it would be useful to be able to form arrays of gels bearing
Lumen Formation Is an Intrinsic Property of Isolated Human Pluripotent Stem Cells.
Journal:
PubMed ID:26626176
'We demonstrate that dissociated human pluripotent stem cells (PSCs) are intrinsically programmed to form lumens. PSCs form two-cell cysts with a shared apical domain within 20 hr of plating; these cysts collapse to form monolayers after 5 days. Expression of pluripotency markers is maintained throughout this time. In two-cell cysts,
Epithelial-mesenchymal transitioned circulating tumor cells capture for detecting tumor progression.
Journal:
PubMed ID:25516888
This study aimed to detect cell-surface vimentin (CSV) on the surface of epithelial-mesenchymal transitioned (EMT) circulating tumor cells (CTC) from blood of patients with epithelial cancers.
Induced pluripotent stem cell-derived mesenchymal stem cell seeding on biofunctionalized calcium phosphate cements.
Journal:
PubMed ID:24839581
Induced pluripotent stem cells (iPSCs) have great potential due to their proliferation and differentiation capability. The objectives of this study were to generate iPSC-derived mesenchymal stem cells (iPSC-MSCs), and investigate iPSC-MSC proliferati on and osteogenic differentiation on calcium phosphate cement (CPC) containing biofunctional agents for the first time. Human iPSCs
Neural Differentiation of Spheroids Derived from Human Induced Pluripotent Stem Cells-Mesenchymal Stem Cells Coculture.
Journal:Tissue Eng Part A
PubMed ID:29160172
'Organoids, the condensed three-dimensional (3D) tissues emerged at the early stage of organogenesis, are a promising approach to regenerate functional and vascularized organ mimics. While incorporation of heterotypic cell types, such as human mesenchymal stem cells (hMSCs) and human induced pluripotent stem cells (hiPSCs)-derived neural progenitors aid neural organ development,