Geltrex™ Reduced-Growth Factor Basement-Membrane Matrix, LDEV-free, stem-cell qualified
DISCONTINUATION NOTICE: Geltrex™ Stem Cell Qualified Matrices won't be restocked — switch to Geltrex™ Flex Stem Cell Qualified Matrices with equivalent performance, expanded vial sizes, and a NEW organoid-qualified option.
This product is a replacement for Geltrex™ hESC-qualified Reduced Growth Factor Basement Membrane Matrix (SKU A1048002)
Geltrex™ Reduced-Growth Factor Basement-Membrane Matrix, LDEV-free, stem-cell qualified
Gibco™

Geltrex™ Reduced-Growth Factor Basement-Membrane Matrix, LDEV-free, stem-cell qualified

Geltrex LDEV-free stem cell-qualified reduced-growth factor basement membrane matrix is a soluble form of basement membrane extracted from murine Engelbreth-Holm-SwarmRead more
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Catalog NumberQuantity
A14133025 mL
A14133011 mL
NOTICE: This product will no longer be restocked — switch now to Geltrex™ Flex hESC Qualified matrix, delivering the same trusted performance in expanded vial sizes, along with a new organoid qualified matrix choice.
Catalog number A1413302
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Geltrex LDEV-free stem cell-qualified reduced-growth factor basement membrane matrix is a soluble form of basement membrane extracted from murine Engelbreth-Holm-Swarm (EHS) tumors and is free of viruses, including lactose dehydrogenase elevating virus (LDEV) sometimes found in this type of preparation. Routinely used for attachment and maintenance of human iPSCs or embryonic stem cells, every lot of this matrix is quality control tested using hESCs or iPSCs to help ensure growth and pluripotency of each lot.

Features of Geltrex Reduced-Growth Factor Basement-Membrane Matrix, LDEV-free, stem cell-qualified, include:

  • Every lot qualified using hESC or iPSC, helping reduce the need for customers to lot screen
  • Free of lactose dehydrogenase elevating virus (LDEV), making it safe for all types of cell culture
  • Consistent protein concentration lot-to-lot, helping ensure reproducible results

Gain reliability and consistency in your hESC/iPSC studies

Human hESCs/iPSCs have specific needs for attachment and maintenance and some lots of basement membrane extract may not provide adequate support. Geltrex Reduced-Growth Factor Basement-Membrane Matrix, LDEV-free, stem cell-qualified, has been tested for support of hESC/iPSC growth and pluripotency, minimizing the need to test multiple lots to find one that works. Our consistent manufacturing process helps ensure a protein concentration with very little variation from lot-to-lot, so you can have confidence in every purchase.

Free of LDEV contamination

Recent advances in processing of EHS tumor cells have resulted in virus-free basement membrane extracts. Every lot is tested by tested by RT-PCR methods so you can be confident that every lot is virus-free.

For Research Use Only. Not for use in diagnostic procedures.
Specifications
Cell TypeHuman induced pluripotent stem cells (iPSC), primary epithelial cells, endothelial cells, smooth muscle cells
Concentration12 to 18 mg/mL
Endotoxin LevelLow
FormatTube(s)
Product LineGibco™
Product TypeBasement Membrane Matrix
Purity or Quality GradeCell Culture Grade
Quantity5 mL
Shelf Life36 months
Shipping ConditionDry Ice
SpeciesMouse
ClassificationReduced Growth Factor
Culture TypeAdherent Cell Culture
FormFrozen
Serum LevelSerum-free
SterilitySterile
Without AdditivesNo Phenol Red
Unit SizeEach
Contents & Storage
Store at -20°C.

Frequently asked questions (FAQs)

Can I use the Geltrex hESC-Qualified, Ready-To-Use, Reduced Growth Factor Basement Membrane Matrix (Cat. No. A1569601) with other PSC media systems (i.e. StemFlex, Essential 8, Essential 8 Flex, etc.)?

No, the Ready-To-Use Geltrex matrix was developed for use with the StemPro hESC SFM System. It is not recommended for other PSC media systems.

For other PSC media systems, we recommend the Geltrex LDEV-Free, hESC-Qualified, Reduced Growth Factor Basement Membrane Matrix (Cat. Nos. A1413301, A1413302).

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What is the difference between Geltrex LDEV-Free and Geltrex hESC-Qualified, Ready-To-Use, Reduced Growth Factor Basement?

Geltrex hESC-Qualified, Ready-To-Use, Reduced Growth Factor Basement Membrane Matrix (Cat. No. A1569601) is a pre-diluted Geltrex product ready to use for coating culture vessels. There is no need to dilute the solution further. The product has also been QC tested using hESCs to ensure that every lot will maintain the attached pluripotency of these cells. The full product description can be found here:

Geltrex hESC-Qualified, Ready-To-Use, Reduced Growth Factor Basement Membrane Matrix (Cat. No. A1569601)

Geltrex LDEV-Free, hESC-Qualified, Reduced Growth Factor Basement Membrane Matrix (Cat. Nos. A1413301, A1413302) is a "concentrated" version of the Geltrex hESC-Qualified, Ready-To-Use above. You can pre-dilute this concentrated solution to pre-coat the culture vessel to grow hESCs at your desired usage concentration. This Geltrex matrix has also been QC tested using hESCs to ensure every lot will maintain the attached pluripotency of these cells. The full product description can be found here:

Geltrex LDEV-Free, hESC-Qualified, Reduced Growth Factor Basement Membrane Matrix (Cat. Nos. A1413301, A1413302)

Geltrex LDEV-Free Reduced Growth Factor Basement Membrane Matrix (Cat. Nos. A1413201, A1413202) is the same as the product above. However, it has not been tested to verify it will support hESC growth and expansion. This is a "general" version and not specific to a cell type or line. A full product description can be found here:

Geltrex LDEV-Free Reduced Growth Factor Basement Membrane Matrix (Cat. Nos. A1413201, A1413202)

Geltrex LDEV-Free Reduced Growth Factor Basement Membrane Matrix (Cat. Nos. A1413201, A1413202)

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

How should I prepare Geltrex LDEV-Free, hESC-Qualified, Reduced Growth Factor Basement Membrane Matrix (Cat. No. A1413301, A1413302) stock solution, and how long is the stock solution stable after thawing?

We recommend thawing Geltrex LDEV-Free, hESC-Qualified, Reduced Growth Factor Basement Membrane Matrix (Cat. No. 1413301, 1413302) at 4 degrees C before making your stock solution. On ice, make a 1:1 dilution with DMEM/F-12 and aliquot into smaller volumes. Stock solution aliquots should be stored at -20 degrees C or used immediately. Once the stock solution is thawed, it should be used for coating plates immediately.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What is the difference between Geltrex LDEV-Free, hESC-Qualified, Reduced Growth Factor Basement Membrane Matrix and Geltrex hESC-Qualified, Ready-To-Use, Reduced Growth Factor Basement Membrane Matrix? I am working with human hepatocytes.

We do not have comparison data for the two Geltrex products. We recommend using the Geltrex LDEV-Free, hESC-Qualified, Reduced Growth Factor Basement Membrane Matrix for hepatocytes. We have not performed a significant amount of testing using the Geltrex hESC-Qualified, Ready-To-Use, Reduced Growth Factor Basement Membrane Matrix on these cells.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What substrate should I use for cardiomyocyte differentiation?

We recommend using Gibco Geltrex LDEV-Free, hESC Qualified Reduced Growth Factor Basement Membrane Matrix (Cat. No. A1413301 or A1413302) or Gibco Vitronectin (VTN-N) Recombinant Human Protein, Truncated (Cat. No. A14700) for xeno-free applications.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

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