Expi293™ Expression Medium

Catalog number: A1435101

Gibco™  Related applications: Antibody & Cell Culture Production | Mammalian Cell Culture | Protein Expression

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Expi293™ Expression Medium is a chemically defined, serum-free, protein-free medium for growth and transfection of suspension-adapted human embryonic kidney (HEK) 293 cells. It is specifically designed as a core component to support high density culture of Expi293F™ cells in the Expi293™ Expression System for scalable transient protein expression.

• Supports growth of suspension 293 cultures to densities of over 1.5 x 10^7 cells/mL
• Transfection compatible serum-free suspension culture medium enables transfection efficiencies of greater than 70% using ExpiFectamine™ transfection reagent
• Enables sustained, high level expression of high density transiently transfected cultures, achieving yields of up to 1 gram per liter of recombinant protein
• Supports growth and transfection of 293 cells in culture formats of less than 1 mL in multiwell plates to greater than 10L in disposable bioreactors
• Does not contain phenol red

Expi293™ Expression Medium is formulated with GlutaMAX™-I reagent. It is ready to use, with no supplementation required. The chemically defined formulation results in high reproducibility and lot-to-lot reliability. Expi293™ Expression Medium contains no human or animal-origin components. The medium is not recommended for adherent cell cultures.
For Research Use Only. Not for use in diagnostic procedures.


Form: Liquid
Format: Bottle(s)
Glucose: High Glucose
Cell Line: 293E (HEK), 293 (HEK), Expi293F™, FreeStyle™ 293-F Cells, 293F
Cell Type: Kidney (Embryonic)
Glutamine: GlutaMAX™-I
Sterility: Sterile
Osmolality: 275-295 mOsm/kg
Serum Level: Serum-Free
Supplements: No Hypoxanthine, No Thymidine, Pyridoxine HCl, Insulin-Free, No 2-Mercaptoethanol, No Vitamin A, Dextrose
Concentrated: 1 X
Culture Type: Suspension Cell Culture
Product Size: 1,000 mL
Classification: Animal Origin-Free, Serum-Free, Protein-Free, Chemically Defined
Endotoxin Level: < 1 EU/ml
Inorganic Salts: Sodium Phosphate, Calcium, Magnesium
Culture Environment: CO2
Included Antibiotics: No Antibiotics
Phenol Red Indicator: No Phenol Red
Purity or Quality Grade: Cell Culture Grade
Sodium Pyruvate Additive: Sodium Pyruvate
Sodium Bicarbonate Buffer: Sodium Bicarbonate
Shipping Condition: Room Temperature

Contents & storage

Store in the dark at +2°C to +8°C


Manuals & protocols

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