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Gibco™

Expi293™ Expression Medium

Catalog number: A1435102
Gibco™

Expi293™ Expression Medium

Catalog number: A1435102
Catalog Number
A1435102
Unit Size
6 x 1 L
Price (USD)
Price: 1,846.00
Your price:
Availability
-
Quantity
Catalog Number
Unit Size
10 L
Price (USD)
Price: 3,000.00
Your price:
Availability
-
Quantity
Catalog Number
Unit Size
20 L
Price (USD)
Price: 5,580.00
Your price:
Availability
-
Quantity
Catalog Number
Unit Size
1,000 mL
Price (USD)
Price: 337.00
Your price:
Availability
-
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
A14351026 x 1 L
Price: 1,846.00
Your price:
-
A143510310 L
Price: 3,000.00
Your price:
-
A143510420 L
Price: 5,580.00
Your price:
-
A14351011,000 mL
Price: 337.00
Your price:
-
Product Overview
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Gibco Expi293 Expression Medium is a chemically defined, serum-free, protein-free medium for growth and transfection of suspension-adapted human embryonic kidney (HEK) 293 cells. It is specifically designed as a core component to support high density culture of Expi293F cells in the Expi293 Expression System for scalable transient protein expression.

• Supports growth of suspension 293 cultures to densities of over 1.5 x 10^7 cells/mL
• Transfection compatible serum-free suspension culture medium enables transfection efficiencies of greater than 70% using ExpiFectamine transfection reagent
• Enables sustained, high level expression of high density transiently transfected cultures, achieving yields of up to 1 gram per liter of recombinant protein
• Supports growth and transfection of 293 cells in culture formats of less than 1 mL in multiwell plates to greater than 10L in disposable bioreactors
• Does not contain phenol red

Expi293 Expression Medium is formulated with GlutaMAX-I reagent. It is ready to use, with no supplementation required. The chemically defined formulation results in high reproducibility and lot-to-lot reliability. Expi293 Expression Medium contains no human or animal-origin components. The medium is not recommended for adherent cell cultures.

Product use
Customers using Expi293 Expression Medium in a manufacturing process, who have a submission with the FDA, may request a letter of authorization from us to reference our Type II Drug Master File (DMF).

For Research use or Further Manufacturing. Not for diagnostic use or direct administration into humans or animals.

Specifications

Culture Environment
CO2
Cell Line
293E (HEK), 293 (HEK), Expi293F™, FreeStyle™ 293-F Cells, 293F
Cell Type
Embryonic Kidney Cells
Classification
Animal Origin-free, Chemically-defined, Protein-free, Serum-free
Concentration
1 X
Culture Type
Suspension Cell Culture
Form
Liquid
Product Type
Expression Medium
Serum Level
Serum-free
Sterility
Sterile
With Additives
GlutaMAX, Sodium Pyruvate, Sodium Bicarbonate, Dextrose, Pyridoxine HCl
Without Additives
No HEPES, No Phenol Red, No Hypoxanthine, No Thymidine, No Insulin, No Vitamin A, No 2-Mercaptoethanol
Endotoxin Level
< 1 EU/ml
Format
Bottle(s)
Included Antibiotics
No antibiotics
Inorganic Salts
Calcium, Magnesium, Sodium Phosphate
Osmolality
275-295 mOsm/kg
Product Line
Expi293™
Purity or Quality Grade
Cell Culture Grade

Contents & Storage

Store in the dark at +2°C to +8°C

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