Alkaline Phosphatase Live Stain

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Description

Alkaline Phosphatase Live Stain is a stem cell imaging product that allows users to differentially stain pluripotent stem cells (PSCs). The dye is a cell-permeable fluorescent substrate for alkaline phosphatase (AP) that is non-toxic to cells, diffusing out over the course of two hours. The Alkaline Phosphatase Live Stain consists of 50 µL of a 500X fluorescein-based dye in DMSO, sufficient for staining two 24-well plates, four 6 cm dishes or two 10 cm dishes.

This Alkaline Phosphatase Live Stain allows you to:

• Identify pluripotent stem cells with a strong signal above background
• Stain PSCs typically in 20 minutes or less
• Use the first alkaline phosphatase stain marketed that maintains cell viability

Alkaline Phosphatase Pluripotent Stem Cell Detection Method
AP is a phenotypic marker of PSCs, including undifferentiated embryonic stem cells (ESCs), induced pluripotent stem cells (iPSCs), and embryonic germ cells (EGCs). While AP is expressed in most cell types, its expression is highly elevated in PSCs. AP staining has therefore been used to differentially stain PSCs to easily distinguish them from mouse embryonic fibroblasts (MEFs) used as feeders and parental fibroblasts commonly used in reprogramming experiments.

Screen for Pluripotency Without Sacrificing Precious Colonies
The Alkaline Phosphatase Live Stain is ideal for screening colonies during early stages of the reprogramming workflow since the stain maintains stem cell viability. It can also be used in later stages as a negative selection tool for identifying undifferentiated cells.

Stain Cells Quickly
The Alkaline Phosphatase Live Stain utilizes an easy, non-permanent, cell-viable protocol for identifying PSCs in your experiments. Simply dilute the dye in media, apply to cells, gently wash, and the cells are ready for fluorescent imaging.

For Research Use Only. Not for use in diagnostics procedures.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Color: Green
For Use With (Equipment): Fluorescence Microscope, Floid® Cell Imaging System
Sub-Cellular Localization: Cell Membranes & Lipids
Form: Liquid
Quantity: 1 tube(s)
Label or Dye: FITC (Fluorescein)
Product Size: 50 µL
Detection Method: Fluorescent
Sample Type (Specific): Live Cells
Shipping Condition: Dry Ice

Contents & storage

Contains 1 amber vial. Store at -20°C.

Documents

Manuals & protocols