CHO CD EfficientFeed™ A AGT™ Nutrient Supplement

Catalog number: A1442001

CHO CD EfficientFeed™ A AGT™ Nutrient Supplement

Catalog number: A1442001
Catalog Number
Unit Size
10 L
Price (USD)
Your Price:
Catalog Number
Unit Size
100 L
Price (USD)
Your Price:
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
A144200110 L
Your Price:
A1442002100 L
Your Price:
Product Overview
Citations & References
Additional Information
Gibco™ CHO CD EfficientFeed™ A nutrient supplement is an animal origin-free, chemically defined complete feed formulation that provides up to 2-5 fold improvement in titers used in fed batch versus batch culture. It contains no proteins, hydrolysates, or components of incompletely defined composition. CHO CD EfficientFeed™ A AGT™ (Advanced Granulation Technology™) Kit Nutrient Supplement provides all the benefits of CHO CD EfficientFeed™ A liquid supplement in a simpler to scale up and reconstitute 2-part kit that is primarily AGT™ dry format material.

The AGT™ format is designed for large scale manufacturing and ease of use. This product is also available in liquid form for small scale use and experimentation.
• Expect up to 2- to 5-fold increases in productivity
• Avoid issues associated with the use of hydrolysates and other alternatives while gaining superior performance
• Simplify scale-up by leveraging the value of AGT™ simple reconstitution
• Achieve performance comparable to original liquid supplement
• Ready for larger scale use with convenient packaging options (10 L and 100 L)

Which EfficientFeed™ Nutrient Supplement to Choose?
Consider the CHO CD Efficient Feed™ Kit when:
• You are using a base medium with less than 6 g/L glucose and no hydrolysate at day 0

Consider CHO CD EfficientFeed™ A supplement when:
• You are currently using CD OptiCHO™ as your base cell growth medium

Consider CHO CD EfficientFeed™ B supplement when:
• You are currently using CD CHO as your base cell growth medium

Consider CD EfficientFeed™ C AGT™ supplement when:
• You want higher productivity in GS CHO and other selected cell lines
• You are currently using CD FortiCHO™ as your base cell growth medium
• You are using a base medium with greater than 6 g/L glucose or a hydrolysate at day 0

Increased Titers
CHO CD EfficientFeed™ supplements have been shown to increase titers 2-5 fold versus batch culture, and are an excellent starting point for process development (Figures 1a & 1b). Furthermore, they outperform competitive alternatives, including less consistent hydrolysates (Figures 2, 3a, & 3b).

Simple Scale-Up and Cost Savings
The ability to scale up in a dry format provides ease of handling and storage in manufacturing and other bioprocess settings. With AGT™ (Advanced Granulation Technology™) dry format, it is possible to store and use the product even more easily and safely than traditional dry format products. CHO CD EfficientFeed™ A AGT™ supplement is offered in 10 L and 100 L packaging that allows scale-up from the 1 L liquid supplement as a seamless process, as well as allow for cost savings when purchasing production level volumes.

Comparable Performance to Liquid Supplement
Equally important as the convenience and simplicity of AGT™ (Advanced Granulation Technology™) dry format is the comparable performance to the liquid CHO CD EfficientFeed™ A version (Figure 4). Both forms have performed similarly in our testing, and the AGT™ version has been used successfully as a prototype in many processes by multiple companies.

AGT™ Format Ease of Use
CHO CD EfficientFeed™ A AGT™ Kit Nutrient Supplement delivers dry format scale-up convenience as well as simple reconstitution with approximately 1% concentrated solution add back. For more details, consult the supporting documents provided below.

For research or further manufacturing use. Not for use in diagnostic procedures.


Product Type
CHO CD Nutrient Supplement
Reconstituted Volume
10 L
Endotoxin Level
Included Antibiotics
No antibiotics
Purity or Quality Grade
Cell Culture Grade, Qualified for Further Manufacturing, Research Grade
Chemically Defined Nutrient Feeding Supplement
Shipping Condition
Wet Ice
Sodium Bicarbonate Buffer
Sodium Bicarbonate
Tested For
Product Line
AGT™, EfficientFeed™
Powder (AGT)
No Glutamine
Phenol Red Indicator
No Phenol Red
Securitainer Capped Vial
With Additives
No Insulin
Culture Type
Suspension Cell Culture
Sodium Pyruvate Additive
Sodium Pyruvate
Cell Line
Animal Origin-Free, Chemically Defined, Protein-Free, Serum-Free
Serum Level

Contents & Storage

Store at 2 to 8°C