DMEM, high glucose, no glutamine, no lysine, no arginine This product, and/or its packaging, incorporates one or more environmental/green features. Click on this icon to learn more.

Catalog number: A1443101

Gibco™  Related applications: Mammalian Cell Culture

Error loading your content!

  Catalog number
Select a plan
Unit size
Price ({{currency}}) Qty
{{product.sku}} {{product.sku}}
also known as {{product.formattedSku}} 
{{product.availability.message}}
Pro add-ons

Your on-site stock

›› {{supplyCenter.scName}}({{scProduct.stockOnHand}} In stock)
›› {{supplyCenter.scName}}(Out of stock)
›› {{supplyCenter.scName}}
This item is not currently available on-site. Depending on your Supply Center settings you may be able to add the item to cart above else use the Order Non-Stocked Items' tab on the Supply Center home page.
Back to top

Description

Dulbecco's Modified Eagle Medium (DMEM), high glucose, no glutamine, no lysine, no arginine is a basal cell culture medium for use during SILAC™ protein labeling with stable isotopic labeled lysine and/or arginine. DMEM is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs and smooth muscle cells, as well as cell lines such as HeLa, 293, Cos-7, and PC-12. We offer a variety of Gibco® DMEM modifications for a range of cell culture applications. Find the right formulation using the media selector tool.

This DMEM is modified as follows:

WithWithout
High GlucoseL-glutamine
Phenol RedSodium Pyruvate
HEPES
Lysine
Arginine
The complete formulation is available.

DMEM is unique from other media as it contains 4 times the concentrations of amino acids and vitamins than the original Eagle's Minimal Essential Medium. DMEM was originally formulated with low glucose (1g/L) and sodium pyruvate, but is often used with higher glucose levels, with or without sodium pyruvate. This DMEM was further modified without lysine and arginine.

Product Intended Use
For Research Use Only: Not for use in diagnostic procedures.

cGMP Manufacturing and Quality System
Gibco® DMEM is manufactured at a cGMP compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards.

DMEM contains no proteins, lipids, or growth factors. Therefore, DMEM requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). SILAC™ protein labeling experiments should be conducted using Gibco® Dialyzed FBS. DMEM uses a sodium bicarbonate buffer system (3.7 g/L) and therefore requires a 5-10% CO2 environment to maintain physiological pH.

Specifications

Glutamine: No Glutamine
Phenol Red Indicator: Phenol Red
Form: Liquid
Glucose: High Glucose
Serum Supplementation: Standard Serum Supplementation
HEPES Buffer: No HEPES
Sodium Pyruvate Additive: No Sodium Pyruvate
Amino Acids: No Arginine, No Lysine
Product Size: 500 mL
Sodium Bicarbonate Buffer: Sodium Bicarbonate
Volume (Metric): 500 ml
Green Features: Fewer resources used, Less waste
Shipping Condition: Room Temperature

Contents & storage

Storage conditions: 2-8°C. Protect from light.

Shipping conditions: Ambient

Shelf life: 12 months from date of manufacture

Documents

Manuals & protocols

Recommended products