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Gibco™

RPMI, no methionine

Catalog number: A1451701
Have Questions?
Gibco™

RPMI, no methionine

Catalog number: A1451701
Have Questions?
Catalog Number
A1451701
Unit Size
500 mL
Price (USD)
Availability
-
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
A1451701500 mL
-
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Roswell Park Memorial Institute (RPMI) 1640 medium was originally developed to culture human leukemic cells in suspension and as a monolayer. RPMI 1640 medium has since been found suitable for a variety of mammalian cells including HeLa, Jurkat, MCF-7, PC12, PBMC, astrocytes, and carcinomas. We offer a variety of Gibco™ RPMI 1640 modifications for a range of cell culture applications. Find the right formulation using our media selector tool.


This RPMI medium is modified as follows:
WithWithout
• L-glutamine• Methionine
• Phenol red• HEPES



Gibco™ RPMI 1640 medium is unique from other media because it contains the reducing agent glutathione and high concentrations of vitamins. RPMI 1640 contains biotin, vitamin B12, and PABA, which are not found in Eagle's Minimal Essential Medium or Dulbecco's Modified Eagle Medium. In addition, the vitamins inositol and choline are present in very high concentrations.

Product Intended Use
For Research Use Only. Not intended for animal or human diagnostic or therapeutic use.

RPMI 1640 medium contains no proteins, lipids, or growth factors. Therefore, RPMI 1640 medium requires supplementation, commonly with 10% fetal bovine serum (FBS). RPMI 1640 medium uses a sodium bicarbonate buffer system (2.0 g/L) and therefore requires a 5–10% CO2 environment to maintain physiological pH.

Specifications

Amino Acids
No Methionine
Cell Line
HeLa, Jurkat, MCF-7, PC-12, PBMC, astrocytes, and carcinomas
Cell Type
Leukemic Cells
Classification
Animal Origin-free
Concentration
1 X
Form
Liquid
Product Type
RPMI 1640 Medium (Roswell Park Memorial Institute 1640 Medium)
Serum Level
Standard Serum Supplementation
Shelf Life
12 Months From Date of Manufacture
Sterility
Sterile-filtered
With Additives
Glutamine, Phenol Red, Sodium Bicarbonate
Without Additives
No HEPES, No Sodium Pyruvate
For Use With (Application)
Mammalian Cell Culture
Green Features
Sustainable packaging
Manufacturing Quality
cGMP-compliant under the ISO 13485 standard
Product Line
Gibco™
Quantity
500 mL
Regulatory Status
RUO
Shipping Condition
Room Temperature

Contents & Storage

Storage conditions: 2-8 Deg;C. Protect from light



Shipping conditions: Ambient



Shelf life: 12 months from date of manufacture

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