Informational:Informational:Interested in cGMP-banked Expi293F cells? Click here for product information.
Catalog Number
A14528
Unit Size
6 x 1 mL
Price (USD)
3,970.00
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Unit Size
1 mL
Price (USD)
1,004.00
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A145286 x 1 mL
3,970.00
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A145271 mL
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Expi293F human cells are derived from the 293 cell line, and are a core component of the Expi293 Expression System. They are maintained in suspension culture and will grow to high density in Expi293 Expression Medium. Expi293F cells are highly transfectable and generate superior protein yields compared to standard 293 cell lines in transient protein expression.

Expi293F Cells attributes include:
Suspension culture cells pre-adapted to Expi293 Expression Medium
Demonstrated higher protein expression levels than other 293 cells adapted to Expi293 Expression Medium
Exhibit more rapid cell growth and higher culture viabilities than standard suspension-adapted 293 cells
Consistent transient expression performance of Expi293F cells for over 40 passages beyond thaw
• Also available from a cGMP bank (Cat. No. 100044202)

Frozen cells are supplied in a vial containing 1 ml of cells at 1 x 107 viable cells/ml in Expi293 Expression Medium and 10% DMSO. The cells should be thawed directly into Expi293 Expression Medium. The six-vial multipack eliminates the need to expand and rebank the cells in the lab.

Cells are typically grown in flasks on a shaker platform in a humidified, CO2 cell culture incubator. However, cultures are scalable from volumes of <1 mL in multiwell plates to >10 L in bioreactors. Protein harvest can be performed at 2 to 7 days post-transfection, depending on the protein expressed. For expression of recombinant antibodies, harvest is typically at 5 to 7 days post-transfection. There is no need for culture medium supplementation or batch feeding during expression.

Specifications

Cell Line
Expi293F™
Cell Type
Kidney (Embryonic)
Culture Type
Suspension Cell Culture
Form
Cryopreserved
No. of Cells
1 x 107
Species
Human
Type
Cell
Media Adaptation
Expi293™ Expression Medium
Parental Cells
FreeStyle™ 293-F Cells
Product Line
Expi293™
Quantity
6 x 1 mL
Storage Requirements
Store in liquid nitrogen
System Type
Expi293™ Expression System

Contents & Storage

1 x 107 cells/mL, store in liquid nitrogen

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