Large Vessel Endothelial Supplement (LVES)

Catalog number:  A1460801

 Related applications:

Cell Viability, Proliferation & Function

| Mammalian Cell Culture

| Primary Cell Culture

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Gibco® Large Vessel Endothelial Supplement (LVES) is a 50X, complete, ready-to-use media supplement optimized for use with Gibco® Medium 200 and Medium 200PRF for culture of primary human umbilical vein endothelial cells (HUVEC), human aortic endothelial cells (HAEC), and human pulmonary artery endothelial cells (HPAEC). This blended supplement has been designed to deliver optimal cell growth and to enable superior cell performance for angiogenesis applications, such as cell migration and tube formation. LVES contains fetal bovine serum, hydrocortisone, human epidermal growth factor, basic fibroblast growth factor, heparin, and ascorbic acid.

• Perform tube formation, cell invasion, cell migration, and cell proliferation functional assays
• Achieve superior growth rates of endothelial cells compared to low serum growth supplement (LSGS)
• Expand primary large vessel endothelial cells without genomic alteration

Verified Performance in Key Angiogenesis Applications
Large Vessel Endothelial Supplement is designed to deliver optimal performance in tube formation, cell invasion, cell migration, and cell proliferation assays when used in conjunction with Medium 200, human umbilical vein endothelial cells, and Geltrex® LDEV-Free Reduced Growth Factor Basement Membrane Matrix. These items and a detailed protocol are available in a convenient Angiogenesis Starter Kit or they can be purchased separately.

Maximum Primary Cell Growth with One Blended Supplement
Large Vessel Endothelial Supplement is a complete, ready-to-use blend of growth supplements optimized to deliver>4 x 104 HUVEC per cm2 when used with Medium 200 or Medium 200PRF. Cell phenotype is retained and verified positive for von Willebrand factor (vWf) and CD31, negative for a-actin, and positive for dil-Ac-LDL uptake via ICC.

For Research Use Only. Not intended for human or animal therapeutic or diagnostic use.
For Research Use Only. Not for use in diagnostic procedures.


Cell Type: Endothelial Cells (Aortic), Endothelial Cells (Large Vessel), Endothelial Cells (Pulmonary Artery), Endothelial Cells (Umbilical Vein)
Classification: Animal Origin
Concentrated: 50 X
Form: Liquid
Glutamine: No Glutamine
Product Size: 11 mL
Purity or Quality Grade: Research Grade
Serum Level: Standard Serum
Species: Human
Sterility: Sterile
Tests Performed: Growth Performance Assay
Volume (Metric): 11 ml
Shipping Condition: Dry Ice

Contents & storage

1 bottle. Store in freezer (-5 to -30°C).


Manuals & protocols