Informational:This product is now part of a food safety range that includes Thermo Scientific™ culture media, molecular solutions, and other products for your complete microbiological workflow. In US, Canada and Europe, order this product from Thermo Fisher Scientific microbiology. >> Order now
The Dynabeads MAX EPEC/VTEC O45 Kit allows for the rapid, selective concentration of E. coli O45 serogroup bacteria in food and environmental samples to improve downstream detection via PCR or culture methods.
• Improve detection sensitivity in PCR sample analysis • Reduce background in selective agar detection • Perform the method manually or automate using the Thermo Scientific KingFisher mL Food Protection Purification System
Improve Sensitivity and Reduce Background Highly specific antibodies attached to paramagnetic beads are incubated with an aliquot of pre-enriched sample. The beads bind and concentrate the target bacteria from non-target organisms. Washing steps help limit the carryover of inhibitors and matrix-associated particulates. These steps combine to improve the sensitivity of PCR-based detection while reducing background for plating methods.
Sensitivity Following the Dynabeads MAX EPEC/VTEC O45 protocol, the presence or absence of one viable E. coli O45 can be determined in the sample sizes described if this one E. coli cell is able to replicate and is not masked by resident background flora.
Specifications
Product Type
EPEC/VTEC Kit
For Use With (Equipment)
Dynabeads™ MPC™, KingFisher mL Food Protection Purification System