The pcDNA™ 3.4 TOPO® vector is a constitutive mammalian expression vector designed to deliver exceptionally high levels of transgene expression. It can be used in suspension-adapted cells, such as Expi293F™, FreeStyle™ 293-F, and FreeStyle™ CHO-S cells, for transient protein expression. The vector can also be used as a Geneticin®-selectable expression plasmid for engineering stable cell lines. The enhanced, full-length CMV promoter and other expression elements typically enable higher expression levels than pcDNA™ 3.1-, 3.2-, and 3.3-based expression constructs.
The pcDNA 3.4 TOPO® vector contains the following elements:
• Full length human cytomegalovirus (CMV) immediate-early promoter/enhancer for high level gene expression in a wide range of mammalian cells • WPRE (woodchuck posttranscriptional regulatory element) downstream of the cloning site to enhance transcript expression • TOPO® cloning site for rapid and efficient (>85%) cloning of Taq-amplified PCR products • Neomycin resistance gene for selection of stable cell lines with Geneticin® • pUC origin for high copy replication and maintenance of the plasmid in E. coli
The pcDNA3.4™ TOPO® TA Cloning Kit includes TOPO®-adapted plasmid vector, reagents for PCR cloning of your gene of choice into the vector, a sequencing primer for confirmation of insert orientation, and One Shot® TOP10 competent cells.
For Research Use Only. Not for use in diagnostic procedures.
Constitutive or Inducible System
TA Cloning Kit
Selection Agent (Eukaryotic)
Antibiotic Resistance Bacterial
Protein Production, Constitutive Expression
High Copy Plasmid
Selection Marker Promoter
Selection Marker Eukaryotic
Contents & Storage
Store pcDNA™3.4-TOPO™ vector and all cloning reagents at -20°C. Store One Shot™ TOP10 Chemically Competent E. coli cells at -80°C.