Invitrogen™

pcDNA™3.4 TOPO™ TA Cloning Kit

Catalog number:  A14697

Related applications:

Antibody & Cell Culture Production

| Protein Expression

| Transfection

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Description

The pcDNA™ 3.4 TOPO® vector is a constitutive mammalian expression vector designed to deliver exceptionally high levels of transgene expression. It can be used in suspension-adapted cells, such as Expi293F™, FreeStyle™ 293-F, and FreeStyle™ CHO-S cells, for transient protein expression. The vector can also be used as a Geneticin®-selectable expression plasmid for engineering stable cell lines. The enhanced, full-length CMV promoter and other expression elements typically enable higher expression levels than pcDNA™ 3.1-, 3.2-, and 3.3-based expression constructs.

The pcDNA 3.4 TOPO® vector contains the following elements:

Full length human cytomegalovirus (CMV) immediate-early promoter/enhancer for high level gene expression in a wide range of mammalian cells
WPRE (woodchuck posttranscriptional regulatory element) downstream of the cloning site to enhance transcript expression
TOPO® cloning site for rapid and efficient (>85%) cloning of Taq-amplified PCR products
Neomycin resistance gene for selection of stable cell lines with Geneticin®
pUC origin for high copy replication and maintenance of the plasmid in E. coli

The pcDNA3.4™ TOPO® TA Cloning Kit includes TOPO®-adapted plasmid vector, reagents for PCR cloning of your gene of choice into the vector, a sequencing primer for confirmation of insert orientation, and One Shot® TOP10 competent cells.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Antibiotic Resistance Bacterial: Ampicillin (AmpR)
Cloning Method: TOPO®-TA
Key Functions: Protein Production, Constitutive Expression
Mass: 20 µg
Plasmid: High Copy Plasmid
Product Line: TOPO®
Replication Origin: pUC Origin
Selection Marker Eukaryotic: NeoR
Selection Marker Promoter: SV40 Promoter
Special Elements: WPRE
Structure: Linear
Constitutive or Inducible System: Constitutive
Delivery Type: Transfection
Promoter: CMV
Selection Agent (Eukaryotic): Geneticin® (G-418)
Vector: pcDNA
Protein Tag: Untagged

Contents & storage

Store pcDNA™3.4-TOPO® vector and all cloning reagents at -20°C.
Store One Shot® TOP10 Chemically Competent E. coli cells at -80°C.