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Invitrogen™

pcDNA™3.4 TOPO™ TA Cloning Kit

Catalog number: A14697
Invitrogen™

pcDNA™3.4 TOPO™ TA Cloning Kit

Catalog number: A14697
Catalog Number
A14697
Unit Size
1 kit
Price (USD)
1,240.00
Your Price:
Availability
***
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
A146971 kit
1,240.00
Your Price:
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Product Overview
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The pcDNA™ 3.4 TOPO® vector is a constitutive mammalian expression vector designed to deliver exceptionally high levels of transgene expression. It can be used in suspension-adapted cells, such as Expi293F™, FreeStyle™ 293-F, and FreeStyle™ CHO-S cells, for transient protein expression. The vector can also be used as a Geneticin®-selectable expression plasmid for engineering stable cell lines. The enhanced, full-length CMV promoter and other expression elements typically enable higher expression levels than pcDNA™ 3.1-, 3.2-, and 3.3-based expression constructs.

The pcDNA 3.4 TOPO® vector contains the following elements:

Full length human cytomegalovirus (CMV) immediate-early promoter/enhancer for high level gene expression in a wide range of mammalian cells
WPRE (woodchuck posttranscriptional regulatory element) downstream of the cloning site to enhance transcript expression
TOPO® cloning site for rapid and efficient (>85%) cloning of Taq-amplified PCR products
Neomycin resistance gene for selection of stable cell lines with Geneticin®
pUC origin for high copy replication and maintenance of the plasmid in E. coli

The pcDNA3.4™ TOPO® TA Cloning Kit includes TOPO®-adapted plasmid vector, reagents for PCR cloning of your gene of choice into the vector, a sequencing primer for confirmation of insert orientation, and One Shot® TOP10 competent cells.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Product Line
TOPO™
Constitutive or Inducible System
Constitutive
Delivery Type
Transfection
Promoter
CMV
Product Type
TA Cloning Kit
Selection Agent (Eukaryotic)
Geneticin™ (G-418)
Antibiotic Resistance Bacterial
Ampicillin (AmpR)
Key Functions
Protein Production, Constitutive Expression
Mass
20 µg
Plasmid
High Copy Plasmid
Replication Origin
pUC Origin
Selection Marker Promoter
SV40 Promoter
Selection Marker Eukaryotic
NeoR
Special Elements
WPRE
Structure
Linear
Protein Tag
Untagged
Cloning Method
TOPO™-TA
Vector
pcDNA

Contents & Storage

Store pcDNA™3.4-TOPO™ vector and all cloning reagents at -20°C.
Store One Shot™ TOP10 Chemically Competent E. coli cells at -80°C.

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