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Invitrogen™

pcDNA™3.4 TOPO™ TA Cloning Kit

Catalog number: A14697
Have Questions?
Invitrogen™

pcDNA™3.4 TOPO™ TA Cloning Kit

Catalog number: A14697
Have Questions?
Catalog Number
A14697
Unit Size
1 kit
Price (USD)
Availability
-
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
A146971 kit
-
Product Overview
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The pcDNA™ 3.4 TOPO™ vector is a constitutive mammalian expression vector designed to deliver exceptionally high levels of transgene expression. It can be used in suspension-adapted cells, such as Expi293F™, FreeStyle™ 293-F, and FreeStyle™ CHO-S cells, for transient protein expression. The vector can also be used as a Geneticin™-selectable expression plasmid for engineering stable cell lines. The enhanced, full-length CMV promoter and other expression elements typically enable higher expression levels than pcDNA™ 3.1-, 3.2-, and 3.3-based expression constructs.

The pcDNA 3.4 TOPO™ vector contains the following elements:

Full length human cytomegalovirus (CMV) immediate-early promoter/enhancer for high level gene expression in a wide range of mammalian cells
WPRE (woodchuck posttranscriptional regulatory element) downstream of the cloning site to enhance transcript expression
TOPO™ cloning site for rapid and efficient (>85%) cloning of Taq-amplified PCR products
Neomycin resistance gene for selection of stable cell lines with Geneticin™
pUC origin for high copy replication and maintenance of the plasmid in E. coli

The pcDNA3.4™ TOPO™ TA Cloning Kit includes TOPO™-adapted plasmid vector, reagents for PCR cloning of your gene of choice into the vector, a sequencing primer for confirmation of insert orientation, and One Shot™ TOP10 competent cells.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Antibiotic Resistance Bacterial
Ampicillin (AmpR)
Cloning Method
TOPO™-TA
Constitutive or Inducible System
Constitutive
Delivery Type
Transfection
For Use With (Application)
Protein Production, Constitutive Expression
Plasmid
High Copy Plasmid
Product Line
TOPO™
Product Type
TA Cloning Kit
Promoter
CMV
Protein Tag
Untagged
Quantity
20 μg
Replication Origin
pUC Origin
Selection Agent (Eukaryotic)
Geneticin™ (G-418)
Selection Marker Eukaryotic
NeoR
Selection Marker Promoter
SV40 Promoter
Vector
pcDNA

Contents & Storage

Store pcDNA™3.4-TOPO™ vector and all cloning reagents at -20°C.
Store One Shot™ TOP10 Chemically Competent E. coli cells at -80°C.

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