GeneArt™ Gene Synthesis Kit

Catalog number:  A14971

 Related applications:

PCR Cloning

| Seamless Cloning & Genetic Assembly

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The new GeneArt® Gene Synthesis Kit with CorrectASE™ technology provides you with all of the proven, high quality reagents necessary for successful do-it-yourself gene synthesis. CorrectASE™ enzyme removes mismatches caused by oligonuceotides errors and facilitates a 3–10 fold reduction of mutations in your synthetic genes or fragments.

The GeneArt® Gene Synthesis Kit:

• Increases the success rate of do-it-yourself gene synthesis by providing standardized reagents and protocol for the complete gene synthesis workflow
• Increases by 3–10 fold the probability of isolating a synthetic gene with correct sequence by including an error correction step into the workflow
• Enables gene synthesis in 3 days, from oligo assembly to sequence verified clone
• Reduces your labor time and sequencing costs by sequencing only 2–4 instead of 10–16 clones

Complete Workflow Solution for Do-it-yourself Gene Synthesis
The GeneArt® Gene Synthesis Kit contains all reagents required for successful do-it-yourself gene synthesis, including:

• High-fidelity Platinum® Pfx DNA Polymerase with automatic hot start for oligonucleotide assembly and amplification
• Quant-iT™ PicoGreen® dsDNA Reagent , an ultra-sensitive, fluorescent nucleic acid stain for quantification of double-stranded DNA (dsDNA)
• pCR™-Blunt II- TOPO® vector for synthetic gene cloning
• CorrectASE™ enzyme, our sequence error correction technology

Prevent Unwanted Mutations
Commercially available synthetic oligonuceotides have a high error rate during synthesis, ranging from one every 300–1000 bases, depending on the source. These errors cause frameshift (deletion and insertion) and mismatch mutations during gene synthesis. Incubation with CorrectASE™ enzyme removes both types of mutations.

The incubation step with CorrectASE™ enzyme is introduced after the initial PCR assembly of oligonucleotides. The PCR product is denatured and reannealed so that any mutations will be unmatched. CorrectASE™ enzyme binds to the resulting mismatches and nicks both DNA strands 3’ of the error. The 3’to 5’ exonuclease activity of the enzyme removes the errors. A final PCR with a proofreading polymerase then assembles the corrected fragments, thus increasing the likelihood of isolating clones with the correct sequence. Depending upon the incoming oligonuceotide quality, only 2–4 clones need to be screened, compared to 10–16 clones in a workflow that does not include the correction step. Including CorrectASE™ enzyme in your gene synthesis workflow decreases labor time and sequencing costs.

For Research Use Only. Not for animal or human therapeutic or diagnostic use.
For Research Use Only. Not for use in diagnostic procedures.


Bacterial or Yeast Strain: TOP10
Cloning Method: Blunt TOPO®
Maximum Size: 1.2 kb (max. insert size)
Number of Fragments: 1 Fragment
Polymerase: Platinum® Pfx DNA Polymerase
Product Size: 10 reactions
Workflow Step: PCR Subcloning

Contents & storage

BOX 1 (-20°C):
Platinum® Pfx DNA Polymerase, 5X Platinum® Pfx PCR Buffer, 10 MM DNTP MIX, 50 mM MgSO4, CorrectASE, 10X CorrectASE Rxn Buffer, 5 mM EDTA, pCR Blunt II-TOPO, TOPO salts, TA-water, Transformation control vector, CAT-oligos, Cat PCR primers, M13 forward sequencing primer, and M13 reverse sequencing primer

BOX2 (RT):
• Q26394, PicoGreen dsDNA reagent (100 uL), qty = 1
• C47262, 20X TE (1 mL), qty = 2
• C47261, Lambda DNA (100 uL), qty = 1

BOX3 (-80°C):
• 460700, SOC Media (7 mL), qty = 1
• 500257, Top10-One Shot, qty = 11
• 54357, pUC19 DNA, qty = 1
• C4040.PPS, product information sheet, qty = 1


Manuals & protocols