Invitrogen™
Neurite Outgrowth Staining Kit
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Description
The Molecular Probes® Neurite Outgrowth Staining Kit allows for quick and simple measurement of neurite outgrowth and cell viability in the same sample. It includes three fluorescent dyes: a cell viability indicator, a cell membrane stain, and a background suppression reagent. Please note that the dyes included in the kit are not specific to neurons, and will stain any animal cells. This kit is not suitable for distinguishing neurons from other cell types in a mixed population.• Stain cells faster than with immunocytochemistry (in as little as 15 minutes)
• Measure neurite outgrowth and cell health in the same sample
• Image either live or fixed cells
• Capture data with a standard fluorescence microscope, microplate reader, or HCS instrument
Rapid Staining Results
The Neurite Outgrowth Staining Kit saves time and steps relative to immunostaining. A typical workflow using this kit takes 15–30 minutes and involves only two media-exchange steps prior to taking a measurement. In contrast, immunostaining involves multiple block, wash, and antibody incubation steps that require several hours to complete.
Multiplexed Readouts
The Neurite Outgrowth Staining Kit distinguishes between compound effects on cell viability and neurite outgrowth by providing a dual color readout.
Flexible Protocol
Depending on the cell type of interest, a fixed-cell staining approach may be preferable to live-cell staining. Both protocols are provided in the product manual for maximum flexibility.
Instrument Compatibility
The fluorescence signals generated with the Neurite Outgrowth Staining Kit can be measured with a fluorescence microplate reader, microscope, or HCS instrument for compatibility with your existing workflow. Likewise, the probes used in this kit are compatible with standard FITC (green) and TRITC (orange-red) emission filter sets.
What You Get:
Easily scalable to any culture plate, dish, or slide format, the Neurite Outgrowth Staining Kit comes with reagents sufficient to run 1,000 96-well assays, and includes:
• 100 µL of Cell Viability Indicator (1000X)
• 100 µL of Cell Membrane Stain (1000X)
• 2.5 mL of Background Suppression Dye (100X)
For Research Use Only. Not for use in diagnostic procedures.
Figures
Cell viability indicator results with primary rat cortex neurons.
Fixed Neuroscreen™-1 Cells Stained with the Neurite Outgrowth Staining Kit and Read on a Fluorescence Microplate Reader
Overlay image of Neurite Outgrowth Staining Kit applied to primary rat cortex neurons.
Neuroscreen™-1 Cells Labeled with the Neurite Outgrowth Staining Kit
Specifications
| Color: | Green, Orange |
|---|---|
| For Use With (Equipment): | Fluorescence Microscope, Fluorescent Imager, HTS Reader, High Content Instrument, Microplate Reader |
| Sub-Cellular Localization: | Neurons, Plasma Membrane |
| Detection Method: | Fluorescent |
| Excitation⁄Emission (nm): | 495/515 (Cell Viability Indicator), 555/565 (Cell Membrane Stain) |
| Form: | Liquid |
| Product Size: | 1,000 assays |
| Shipping Condition: | Approved for shipment at Room Temperature or on Wet or Dry Ice |
Contents & storage
100 µL 1000X Cell Viability Indicator
100 µL 1000X Cell Membrane Stain
2.5 mL 100X Background Suppression Dye
Each component stored at -20°C.
100 µL 1000X Cell Membrane Stain
2.5 mL 100X Background Suppression Dye
Each component stored at -20°C.
