This kit contains a rabbit Tb-anti-GST antibody—an equivalent kit containing a goat-sourced antibody is also available.

LanthaScreen® TR-FRET RAR beta Coactivator Assay Kit, rabbit

Catalog number: A15137

 Related applications: Pharma & Biopharma | Target & Lead Identification & Validation

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This kit contains Rabbit Tb-Anti-GST antibody; other kit components are the same as kit PV4688:

The LanthaScreen® TR-FRET RAR (retinoic acid receptor) beta Coactivator Assay Kit provides a sensitive and robust method for highthroughput screening of potential RAR beta ligands as agonists of ligand-dependent coactivator recruitment. The kit uses a terbium (Tb)-labeled anti-GST antibody, a fluorescein-labeled coactivator peptide, and a human recombinant RAR beta ligand-binding domain (RAR beta-LBD) that is tagged with glutathione-S-transferase (GST) in a homogeneous, mix-and-read assay format.

When running the LanthaScreen® TR-FRET RAR beta coactivator assay in agonist mode to identify agonist compounds, RAR beta-LBD is added to ligand test compounds, followed by addition of a mixture of the fluorescein-coactivator peptide and Tb-labeled anti-GST antibody. After an incubation period at room temperature, the 520 nm/495 nm TR-FRET ratio is calculated and can be used to determine the EC50 from a dose response curve of the compound. Based on the biology of the RAR beta-coactivator peptide interaction, this ligand EC50 is a composite value representing the amount of ligand required to bind to receptor, effect a conformational change, and recruit coactivator peptide (Figure 1).

Contents and Storage:
The LanthaScreen® TR-FRET RAR beta Coactivator Assay Kit contains RAR beta-LBD (GST) protein, fluorescently labeled SRC2-2 coactivator peptide, Tb-labeled anti-GST antibody, and buffers. Store components as indicated in the assay protocol (-80°C, -20°C, or +4°C).
For Research Use Only. Not for use in diagnostic procedures.


Color: Green
Format: 384-well plate
Readout: TR-FRET (Activity)
Technique: TR-FRET
Label or Dye: Tb (Terbium)
Product Size: 800 x 20 µL assays
Detection Method: Fluorescent
Druggable Target: Nuclear Receptors
Validated Application: Nuclear Receptor Assay
For Use With (Equipment): Microplate Reader

Contents & storage

Tb-anti-GST Antibody (Rabbit): Store at -20 °C
RAR beta-LBD, GST: Store at -80 °C
Fluoroscein Coactivator Peptide: Store at -20 °C
TR-FRET Coregulatore Buffer E: Store at 4 °C
1M DTT: Store at -20 °C or -80 °C


Manuals & protocols