This kit contains a rabbit Tb-anti-GST antibody—an equivalent kit containing a goat-sourced antibody is also available.

LanthaScreen® TR-FRET RXR alpha Coactivator Assay Kit, rabbit

Catalog number: A15138

 Related applications: Pharma & Biopharma | Target & Lead Identification & Validation

Error loading your content!

  Catalog number
Select a plan
Unit size
Price ({{currency}}) Your price ({{currency}}) Availability Qty
{{product.sku}} {{product.sku}}
also known as {{product.formattedSku}} 
{{subscriptionDetails.selectedRatePlan.billingPeriod}} {{subscriptionDetails.selectedRatePlan.billingPeriod}} {{product.availability.message}}
Pro add-ons

Your on-site stock

›› {{supplyCenter.scName}}({{scProduct.stockOnHand}} In stock)
›› {{supplyCenter.scName}}(Out of stock)
›› {{supplyCenter.scName}}
This item is not currently available on-site. Depending on your Supply Center settings you may be able to add the item to cart above else use the Order Non-Stocked Items' tab on the Supply Center home page.
Back to top


This kit contains Rabbit Tb-Anti-GST antibody; other kit components are the same as kit PV4797:

The LanthaScreen® TR-FRET Retinoid X Receptor alpha Coactivator Assay provides a sensitive and robust method for high-throughput screening of potential RXR alpha ligands as agonists of ligand-dependent coactivator recruitment. The kit uses a terbium (Tb)-labeled anti- GST antibody, a fluorescein-labeled coactivator peptide, and a human recombinant RXR alpha ligand-binding domain (RXR alpha-LBD) that is tagged with glutathione-S-transferase (GST) in a homogeneous mix-and-read assay format.

To assay:
When running the LanthaScreen® TR-FRET Retinoid X Receptor alpha Coactivator Assay in agonist mode (to identify agonist compounds), RXR alpha-LBD is added to ligand test compounds followed by addition of a mixture of the fluorescein-coactivator peptide and Tb-labeled anti-GST antibody. After an incubation period at room temperature, the TR-FRET ratio of 520/495 is calculated and can be used to determine the EC50 from a dose-response curve of the compound. Based on the biology of the RXR alpha-coactivator peptide interaction, this ligand EC50 is a composite value representing the amount of ligand required to bind to receptor, effect a conformational change, and recruit coactivator peptide (see Figure 1).
For Research Use Only. Not for use in diagnostic procedures.


Color: Green
Format: 384-well plate
Readout: TR-FRET (Activity)
Technique: TR-FRET
Label or Dye: Tb (Terbium)
Product Size: 800 x 20 µL assays
Detection Method: Fluorescent
Druggable Target: Nuclear Receptors
Validated Application: Nuclear Receptor Assay
For Use With (Equipment): Microplate Reader

Contents & storage

Tb-anti-GST Antibody (Rabbit): Store at -20 °C
RXR alpha-LBD, GST: Store at -80 °C
Fluoroscein Coactivator Peptide: Store at -20 °C
TR-FRET Coregulatore Buffer G: Store at 4 °C
1M DTT: Store at -20 °C or -80 °C


Manuals & protocols