This kit contains Rabbit Tb-Anti-GST antibody; other kit components are the same as kit PV4836:
The LanthaScreen™ TR-FRET Constitutive Androstane Receptor (CAR) Coactivator Assay provides a sensitive and robust method for highthroughput screening (HTS) of potential CAR ligands as agonists of ligand-dependent coactivator recruitment or inverse agonists of liganddependent coactivator displacement. The kit uses a terbium-labeled anti-GST antibody, a fluorescein-labeled coactivator peptide, and a CAR ligand-binding domain (CAR-LBD) that is tagged with glutathione-S-transferase (GST) in a homogeneous mix-and-read assay format.
To assay: When running the LanthaScreen™ TR-FRET CAR Coactivator Assay, CAR-LBD is added to ligand test compounds followed by addition of a mixture of the fluorescein-coactivator peptide and terbium anti-GST antibody. After an incubation period at room temperature, the 520 nm/ 495 nm TR-FRET ratio is calculated and can be used to determine the EC50 from a dose response curve of the compound. Based on the biology of the CAR-coactivator peptide interaction, this ligand’s EC50 is a composite value representing the amount of ligand required to bind to receptor, effect a conformational change, and either recruit or displace coactivator peptide (Figures 1 and 2).
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Color
Green
Conjugate
Tb (Terbium)
Detection Method
Fluorescent
For Use With (Equipment)
Microplate Reader
Packaging
384-well Plate
Assay Category
Biochemical Assay
Druggable Target
Nuclear Receptors
Readout
TR-FRET (Activity)
Product Line
LanthaScreen™
For Use With (Application)
Nuclear Receptor Assay, TR-FRET
Ligand
CAR
Product Type
TR-FRET CAR Coactivator Assay Kit
Contents & Storage
Tb-anti-GST Antibody (Rabbit): Store at -20 °C CAR-LBD, GST: Store at -80 °C Fluoroscein Coactivator Peptide: Store at -20 °C TR-FRET Coregulatore Buffer G: Store at 4 °C 1M DTT: Store at -20 °C or -80 °C