This kit contains a rabbit Tb-anti-GST antibody—an equivalent kit containing a goat-sourced antibody is also available.

LanthaScreen® TR-FRET PXR (SXR) Competitive Binding Assay Kit, rabbit

Catalog number: A15142

 Related applications: Pharma & Biopharma | Target & Lead Identification & Validation

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Description

This kit contains Rabbit Tb-Anti-GST antibody; other kit components are the same as kit PV4839:

The LanthaScreen® TR-FRET PXR (SXR) Competitive Binding Assay Kit provides a sensitive and robust method for high-throughput screening (HTS) of ligands for the pregnane X receptor (PXR), also known as the steroid and xenobiotic receptor (SXR). The kit uses a terbium (Tb)-labeled anti-GST antibody, a fluorescent small-molecule PXR ligand (Fluormone™ PXR (SXR) Green), and a human PXR (SXR) ligand-binding domain that is tagged with glutathione S-transferase (GST) in a homogeneous mix-and-read assay format.

To assay:
When running the LanthaScreen® TR-FRET PXR (SXR) competitive binding assay, a Tb-labeled anti-GST antibody is used to indirectly label the receptor by binding to its GST tag. Competitive ligand binding is detected by a test compound’s ability to displace a fluorescent ligand (tracer) from the receptor, which results in a loss of FRET signal between the Tb-anti-GST antibody and the tracer (Figure 1). This type of binding assay is analogous to radioligand-based assays, except that it eliminates handling of radioactivity and enables a homogeneous, “addition-only " format.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Color: Green
Detection Method: Fluorescent
Druggable Target: Nuclear Receptors
For Use With (Equipment): Microplate Reader
Format: 384-well plate
Label or Dye: Tb (Terbium)
Product Line: LanthaScreen®
Product Size: 800 x 20 µL assays
Readout: TR-FRET (Binding)
Technique: TR-FRET
Validated Application: Nuclear Receptor Assay

Contents & storage

Tb-anti-GST Antibody (Rabbit): Store at -20 °C
PXR(SXR)-LBD, GST: Store at -80 °C
Fluormone PXR(SXR) Green: Store at -20 °C
TR-FRET PXR(SXR) Assay Buffer: Store at 4 °C
1M DTT: Store at -20 °C or -80 °C

Documents

Manuals & protocols