Essential 8™ Medium

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인용 및 참조 문헌 (5)

Gibco™

Essential 8™ Medium

Essential 8™ Medium is a xeno-free and feeder-free medium specially formulated for the growth and expansion of human pluripotent stem자세히 알아보기

카탈로그 번호	수량
A1517001	500 mL

카탈로그 번호 A1517001

제품 가격(KRW)

323,000

온라인 행사

Ends: 31-Mar-2026

358,000

할인액 35,000 (10%)

카트에 추가하기

500 mL

제품 가격(KRW)

323,000

온라인 행사

Ends: 31-Mar-2026

358,000

할인액 35,000 (10%)

카트에 추가하기

Essential 8™ Medium is a xeno-free and feeder-free medium specially formulated for the growth and expansion of human pluripotent stem cells (PSCs). Originally developed by Guokai Chen et al. in the laboratory of James Thomson (published as 'E8') and validated by Cellular Dynamics International, Essential 8™ Medium has been extensively tested and proved to maintain pluripotency in multiple iPSC lines. In addition, Essential 8™ Medium has been used to scale up production of iPSCs and shown to support iPSC growth for >50 passages without any signs of karyotypic abnormalities, along with maintaining the ability of iPSCs to differentiate into all three germ line lineages.

Features of Essential 8™ Medium include:

• Consistent—reduced variability compared to existing feeder-free culture media
• Robust—reliable and robust cultures with a xeno-free, cGMP, 8-component medium
• Cost effective—economical and scalable PSC culture compared to other feeder-free media

Reduced Variability
Essential 8™ Medium is xeno-free and contains only the 8 essential components needed for stem cell culture. Unlike other media that contain over 20 highly variable ingredients, Essential 8™ Medium is produced under cGMP and has an optimized formulation and growth factor levels to help ensure maximum cell health, pluripotency, and growth, with minimal variability.

Reliable and Robust Cultures
Essential 8™ Medium has been show to support pluripotent stem cell growth and provide cultures with superior morphology and growth kinetics compared to other feeder systems.

Cost Effective
Essential 8™ Medium is provided in a convenient two-component kit (500 ml basal medium & 10 ml supplement), and when used with vitronectin (VTN-N), provides a cost effective, defined system for feeder-free culture of human pluripotent stem cells (PSCs).

Essential 8™ Medium is commercialized in partnership with Cellular Dynamics International.

For Research Use Only. Not for use in diagnostic procedures.

세포주Embryonic Stem Cells (ESCs), Induced Pluripotent Stem Cells (iPSCs)

세포 유형Embryonic Stem Cells (ESCs), Induced Pluripotent Stem Cells (iPSCs)

농도Essential 8 Basal Medium (1X), Essential 8 Supplement (50X)

제조 품질cGMP for medical devices, 21 CFR Part 820 and ISO 13485

제품라인Essential 8

제품 유형Stem Cell Media

수량500 mL

유통 기한12 Months

배송 조건Supplement - dry ice, Basal - ambient

종Human

분류Xeno-free

Culture TypeAdherent Cell Culture, Feeder-free Stem Cell Culture (Human, iPS - Induced Pluripotent Stem, Embryonic), Stem Cell (Human, iPS - Induced Pluripotent Stem, Embryonic)

형태Liquid

Serum LevelSerum-free

멸균Sterile-filtered

Sterilization MethodSterile-filtered

첨가제 포함Phenol Red

Unit SizeEach

구성 및 보관

• 500 mL basal medium (store at 2—8°C and protect from light)
• 10 mL supplement (store at -5 to -20°C and protect from light)

자주 묻는 질문(FAQ)

Do you still recommend using Gibco Vitronectin as the matrix for Gibco Essential 8 Flex Medium?

Both Gibco Vitronectin (VTN-N) Recombinant Human Protein, Truncated (Cat. No. A400457 and A400458) and Gibco Geltrex Flex LDEV-Free hESC-qualified Reduced Growth Factor Basement Membrane Matrix (Cat. No. A4000046801, A4000046802, or A4000046803) have been successfully used with Essential 8 Flex Medium.

Can PSCs transitioned over into Essential 8 Medium on recombinant human laminin-521 (rhLaminin-521) from feeder-dependent cultures, then be transferred onto vitronectin (VTN-N)?

Yes. Following 2 passages on the rhLaminin-521 matrix, Versene or EDTA passaging should be used to subculture PSCs.

What is Gibco Vitronectin (VTN-N)?

Gibco Vitronectin (VTN-N) is a recombinant, truncated human protein corresponding to the amino acid fragment 62-478 of human vitronectin expressed in E. coli. When used with Gibco Essential 8 Medium, VTN-N has been demonstrated to maintain pluripotency and normal growth characteristics in multiple PSC lines.

What are the main differences to be taken into consideration when culturing cells in Gibco Essential 8 Medium on Gibco Vitronectin (VTN-N) compared to other feeder-free systems?

Here are three major differences to be taken into consideration when culturing cells in Gibco Essential 8 Medium on Gibco Vitronectin (VTN-N) compared to other feeder-free systems:
- Cells should be typically passaged ~24 hours sooner than they would be with other feeder-free media.
- Passaging should take place when cells are at ~85% confluency. If cells are passaged when they are more than 85% confluent, the health of the cells and final cell yield may be compromised.
- Cells must be passaged in EDTA. Collagenase and dispase are not recommended.

Can Gibco Essential 8 Medium and Gibco Vitronectin (VTN-N) support long-term growth of pluripotent stem cells (PSCs)?

Gibco Essential 8 Medium and vitronectin have been shown to support PSC growth for >50 passages without any signs of karyotypic abnormalities, and maintain the ability of PSCs to differentiate into all three germ line lineages. As published by Chen et al (http://www.ncbi.nlm.nih.gov/pubmed/21478862) in the laboratory of James Thomson, the VTN-N variant of vitronectin supports human pluripotent stem cell attachment and survival better than wild-type vitronectin when used in conjunction with Gibco Essential 8 Medium.

View all Essential 8™ Medium FAQs

인용 및 참조 문헌 (5)

인용 및 참조 문헌

Abstract

A comprehensive protocol for efficient differentiation of human NPCs into electrically competent neurons.

Authors:Romito E,Battistella I,Plakhova V,Paplekaj A,Forastieri C,Toffolo E,Musio C,Conti L,Battaglioli E,Rusconi F

Journal:Journal of neuroscience methods

PubMed ID:39053772

Inhibition of SARS-CoV-2 Infections in Engineered Human Tissues Using Clinical-Grade Soluble Human ACE2.

Authors:Monteil V, Kwon H, Prado P, Hagelkrüys A, Wimmer RA, Stahl M, Leopoldi A, Garreta E, Hurtado Del Pozo C, Prosper F, Romero JP, Wirnsberger G, Zhang H, Slutsky AS, Conder R, Montserrat N, Mirazimi A, Penninger JM

Journal:Cell

PubMed ID:32333836

'We have previously provided the first genetic evidence that angiotensin converting enzyme 2 (ACE2) is the critical receptor for severe acute respiratory syndrome coronavirus (SARS-CoV), and ACE2 protects the lung from injury, providing a molecular explanation for the severe lung failure and death due to SARS-CoV infections. ACE2 has now ... More

Scalable topographies to support proliferation and Oct4 expression by human induced pluripotent stem cells.

Authors:Reimer A, Vasilevich A, Hulshof F, Viswanathan P, van Blitterswijk CA, de Boer J, Watt FM,

Journal:Sci Rep

PubMed ID:26757610

It is well established that topographical features modulate cell behaviour, including cell morphology, proliferation and differentiation. To define the effects of topography on human induced pluripotent stem cells (iPSC), we plated cells on a topographical library containing over 1000 different features in medium lacking animal products (xeno-free). Using high content ... More

Defined Essential 8™ Medium and Vitronectin Efficiently Support Scalable Xeno-Free Expansion of Human Induced Pluripotent Stem Cells in Stirred Microcarrier Culture Systems.

Authors:Badenes SM, Fernandes TG, Cordeiro CS, Boucher S, Kuninger D, Vemuri MC, Diogo MM, Cabral JM,

Journal:PLoS One

PubMed ID:26999816

Human induced pluripotent stem (hiPS) cell culture using Essential 8™ xeno-free medium and the defined xeno-free matrix vitronectin was successfully implemented under adherent conditions. This matrix was able to support hiPS cell expansion either in coated plates or on polystyrene-coated microcarriers, while maintaining hiPS cell functionality and pluripotency. Importantly, scale-up ... More

A Human Pluripotent Stem Cell-based Platform to Study SARS-CoV-2 Tropism and Model Virus Infection in Human Cells and Organoids.

Authors:Yang L, Han Y, Nilsson-Payant BE, Gupta V, Wang P, Duan X, Tang X, Zhu J, Zhao Z, Jaffré F, Zhang T, Kim TW, Harschnitz O, Redmond D, Houghton S, Liu C, Naji A, Ciceri G, Guttikonda S, Bram Y, Nguyen DT, Cioffi M, Chandar V, Hoagland DA, Huang Y, Xiang J, Wang H, Lyden D, Borczuk A, Chen HJ, Studer L, Pan FC, Ho DD, tenOever BR, Evans T, Schwartz RE, Chen S

Journal:Cell Stem Cell

PubMed ID:32579880

SARS-CoV-2 has caused the COVID-19 pandemic. There is an urgent need for physiological models to study SARS-CoV-2 infection using human disease-relevant cells. COVID-19 pathophysiology includes respiratory failure but involves other organ systems including gut, liver, heart, and pancreas. We present an experimental platform comprised of cell and organoid derivatives from ... More