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Catalog Number
A1517001
Unit Size
500 mL
Price (USD)
Price: 296.00
Online price: 259.65
Your price:
Availability
-
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
A1517001500 mL
Price: 296.00
Online price: 259.65
Your price:
-
Product Overview
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Essential 8™ Medium is a xeno-free and feeder-free medium specially formulated for the growth and expansion of human pluripotent stem cells (PSCs). Originally developed by Guokai Chen et al. in the laboratory of James Thomson (published as 'E8') and validated by Cellular Dynamics International, Essential 8™ Medium has been extensively tested and proved to maintain pluripotency in multiple iPSC lines. In addition, Essential 8™ Medium has been used to scale up production of iPSCs and shown to support iPSC growth for >50 passages without any signs of karyotypic abnormalities, along with maintaining the ability of iPSCs to differentiate into all three germ line lineages.

Features of Essential 8™ Medium include:

• Consistent—reduced variability compared to existing feeder-free culture media
• Robust—reliable and robust cultures with a xeno-free, cGMP, 8-component medium
• Cost effective—economical and scalable PSC culture compared to other feeder-free media

Reduced Variability
Essential 8™ Medium is xeno-free and contains only the 8 essential components needed for stem cell culture. Unlike other media that contain over 20 highly variable ingredients, Essential 8™ Medium is produced under cGMP and has an optimized formulation and growth factor levels to help ensure maximum cell health, pluripotency, and growth, with minimal variability.

Reliable and Robust Cultures
Essential 8™ Medium has been show to support pluripotent stem cell growth and provide cultures with superior morphology and growth kinetics compared to other feeder systems.

Cost Effective
Essential 8™ Medium is provided in a convenient two-component kit (500 ml basal medium & 10 ml supplement), and when used with vitronectin (VTN-N), provides a cost effective, defined system for feeder-free culture of human pluripotent stem cells (PSCs).

Essential 8™ Medium is commercialized in partnership with Cellular Dynamics International.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Cell Type
Embryonic Stem Cells (ESCs), Induced Pluripotent Stem Cells (iPSCs) 
Classification
Xeno-free
Culture Type
Feeder-free Stem Cell Culture (Human, iPS - Induced Pluripotent Stem, Embryonic)
Form
Liquid
Product Type
Stem Cell Media
Serum Level
Serum-free
With Additives
Phenol Red
Manufacturing Quality
cGMP for medical devices, 21 CFR Part 820 and ISO 13485
Product Line
Essential 8™
Quantity
500 mL
Shipping Condition
Supplement - dry ice, Basal - ambient
Species
Human

Contents & Storage

• 500 mL basal medium (store at 2—8°C and protect from light)
• 10 mL supplement (store at -5 to -20°C and protect from light)

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