TaqPath™ 1-Step RT-qPCR Master Mix, CG

Catalog number: A15299

Applied Biosystems™  Related applications: Diagnostic Development | Real Time PCR (qPCR) | Real Time PCR-Based Gene Expression Profiling | miRNA & Non-Coding RNA Analysis

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TaqPath™ 1-Step RT-qPCR Master Mix, CG, is designed for robust and reproducible one-step pathogen detection and gene expression workflows. The single-tube 4X formulation contains thermostable MMLV reverse transcriptase, dNTPs, UNG, ROX™ dye, and thermostable Fast DNA polymerase, facilitating easy reaction set up—just add user-supplied assay and sample (Figure 1). All manufacturing lots are functionally tested to ensure remarkable lot-to-lot reproducibility for CT consistency and dynamic range across a wide variety of assays. TaqPath™ 1-Step RT-qPCR Master Mix, CG, is a General Purpose Reagent (GPR), manufactured according to applicable 21 CFR requirements and is labeled "For Laboratory Use". With stringent quality coupled with premier performance, TaqPath™ 1-Step RT-qPCR Master Mix, CG, is a superior choice for your diagnostic testing or development needs.

Features of the TaqPath™ 1-Step RT-qPCR Master Mix, CG, include:

• High sensitivity to detect low-copy targets with reproducible CT results
• 6 logs of dynamic range with RNA, DNA, and multiplexing applications*
• Tolerance of inhibitors commonly found in clinical samples
• Labeled "For Laboratory Use"

High Sensitivity
With understanding of the importance of reproducible detection for low-titer pathogen or transcripts in clinical diagnostic testing, TaqPath™ 1-Step RT-qPCR Master Mix, CG, has been optimized as a higher-concentration 4X master mix that allows you to input more sample into each reaction, increasing sensitivity even in low-volume reactions. Figure 2 shows consistent CT results obtained from three unique lots when detecting 10 copy inputs of RNA target. Figure 3 demonstrates CT lot-to-lot consistency is preserved across multiple assays with different attributes and expression levels.

Wide Dynamic Range Compatible with RNA, DNA, and Multiplexing
TaqPath™ 1-Step RT-qPCR Master Mix, CG, has been optimized to provide high specificity and dynamic range for use with both RNA and DNA targets. Since virology labs often test for both RNA and DNA viruses, TaqPath™ 1-Step RT-qPCR Master Mix, CG, is designed to use a single protocol to assay both types of nucleic acid. This input flexibility can help streamline the number of different workflows in your lab to improve efficiency. Figure 4 demonstrates the excellent PCR linearity across a 6-log input range for both RNA (Figure 4a) and DNA targets (Figure 4b).

TaqPath™ 1-Step RT-qPCR Master, CG, is compatible with multiplexing reactions, allowing for additional exogenous or endogenous controls or targets to be run simultaneously for quality control or efficiency purposes. Figure 5 demonstrates TaqPath™ 1-Step RT-qPCR Master Mix performance in a triplex reaction. The formulation can be run in either fast or standard cycling conditions with equivalent performance across a wide variety of qPCR platforms.

TaqPath™ 1-Step RT-qPCR Master Mix, CG, has been benchmarked against similar competitor master mixes and demonstrates equivalent or better sensitivity and dynamic range across a variety of targets (Figure 6).

Inhibitor Tolerant
Unlike other master mixes on the market, TaqPath™ 1-Step RT-qPCR Master Mix’s unique proprietary formulation allows robust performance even in the presence of substances that can normally inhibit PCR, such as heparin, hematin, or EDTA, increasing your confidence when working with a variety of complex clinical samples. TaqPath™ master mixes demonstrate increased tolerance of inhibitors compared to competitor mixes (Figure 7).

Manufacturing Production and Process Controls
TaqPath™ qPCR Master Mix is manufactured under a strong quality management system that utilizes traceable quality raw materials and validated operating procedures. With established controls from purchasing through QC release, TaqPath™ product manufacturing is designed to produce consistent product performance lot after lot.

General Purpose Reagent
For customers in the USA, TaqPath™ 1-Step RT-qPCR Master Mix is a General Purpose Reagent and "For Laboratory Use".
For Laboratory Use


PCR Method: 1-Step RT-PCR
Reverse Transcriptase: M-MLV Reverse Transcriptase
Polymerase: Fast DNA Polymerase
Detection Method: Primer-Probe Detection
Primer-Probe Compatibility: TaqMan®
Reaction Speed: Standard, Fast
For Use With (Equipment): 7500 Fast Dx System, Real-Time PCR Systems or Thermal Cyclers, QuantStudio™ Dx
Sample Type (General): RNA, DNA, cDNA
Passive Reference Dye: ROX (Pre-mixed)
Form: Liquid
Format: Tube(s)
Hot Start: Built-In Hot Start
Product Size: 5 x 1 mL
Number of Reactions: 1000 reactions

Contents & storage

Contains five 1-ml tubes of 4X RT-PCR master mix sufficient for 1000 20-µL reactions.
Store in freezer (-5 to -30°C).


Manuals & protocols