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TaqMan® hPSC Scorecard™ Kit, Fast 96-well

Catalog number: A15871

Applied Biosystems™  Related applications: Stem Cell Analysis

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Description

The Applied Biosystems® TaqMan® hPSC Scorecard™ Kit 2 x 96w FAST enables verification of pluripotency and determination of lineage bias for both ES and iPS cell lines. Each of the two 96-well plates contains 94 predefined TaqMan® Gene Expression assays (including endogenous controls) dried-down in the wells. Master mix is included for your convenience. To purchase the panel alone, see TaqMan® hPSC Scorecard™ Panel 2 x 96w FAST. This product is also available in a 384-well format.

• Assess an entire gene signature in one simple experiment
• Compare results to a reference standard with the accompanying hPSC Scorecard™ Analysis Software
• Identify lines with trilineage differentiation potential
• Evaluate inductive differentiation specified to one germ layer
• Access validated content for increased confidence in your results

Assess an Entire Gene Signature in One Experiment
The TaqMan® hPSC Scorecard™ Panel helps save time by offering pre-plated assays in a stable and convenient dried-down format. Simply add TaqMan® Master Mix to your cDNA of interest and transfer to the 96-well plate with a multichannel pipette.

Compare Results to a Reference Standard
The TaqMan® hPSC Scorecard™ Panel includes access to proprietary cloud-based analysis software that allows you to view and export graphs and tables of results, as well as generate reports. The software is compatible with seven Applied Biosystems® qRT-PCR systems and is available at no additional charge.

Identify Lines with Trilineage Differentiation Potential
Include randomly differentiated embryoid body (EB) samples (differentiated for at least 7 days using standard EB methods) in your analysis and determine whether your lines are biased toward one or more of the three germ layers (endoderm, mesoderm, ectoderm).

Evaluate Inductive Differentiation Specified to One Germ Layer
The panel includes over twenty markers for each of the three germ layers that have been shown to respond as expected to directed differentiation in monolayer culture. Quickly evaluate time course data, culture conditions and media formulations for your germ layer of interest with this one panel.

Access Validated Content
The panel contents are based on published work (Bock et al., Cell 144, 439–452, 2011) and were validated against multiple human ES and iPS lines.

What You Get
The TaqMan® hPSC Scorecard™ Panel 2 x 96w FAST includes:

• Two 96-well plates of 94 TaqMan® Gene Expression assays
• Two optical plate covers
• Two 1 mL vials TaqMan® Fast Advanced Master Mix
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Form: Dried Down
Format: 96-well plate
Species: Human
Product Size: 2 x 96 well plates
Reaction Speed: Fast
Detection Method: Primer-Probe Detection
Research Category: Stem Cell Research
Number of Reactions: 96 Reactions
For Use With (Equipment): StepOnePlus™, Fast Mode, QuantStudio™ 12K Flex, ViiA™ 7 System

Contents & storage

• Two 96-well plates each containing the 96-assay panel (room temperature)
• Two optical covers (room temperature storage)
• Two 1 mL vials of TaqMan® Fast Advanced Master Mix (thawed for shipping, DO NOT RE-FREEZE, store at 2–8°C)

Documents

Manuals & protocols