Thermo Scientific™

LanthaScreen™ TR-FRET Glucocorticoid Receptor Competitive Binding Kit

Catalog number:  A15901

Related applications:

Pharma & Biopharma

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Description

The LanthaScreen® TR-FRET Glucocorticoid Receptor Competitive Binding Assay Kit provides a sensitive and robust method for high-throughput screening (HTS) of ligands for glucocorticoid receptor (GR). The kit uses a human GR ligand-binding domain (GR-LBD) tagged with glutathione S-transferase (GST) (also available separately), a terbium-labeled anti-GST antibody, and a fluorescent small-molecule GR ligand (Fluormone™ GS1) in a homogeneous mix-and-read assay format.

The Lanthascreen Gluococorticoid Receptor Competitive Binding Assay is:

• Complete & ready-to-use out of the box—all reagents included, just add your test compounds
• High throughput compatible—TR-FRET assays in a 384-well plate
• Convenient—components available in bulk sizes for larger screening needs
WARNING: Reproductive Harm - www.P65Warnings.ca.gov
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Product Line: LanthaScreen®
Validated Application: Competitive Binding Assay, Nuclear Receptor Assay

Contents & storage

1 tube GR Ligand Binding Domain Recombinant Protein (store at -68 to -85°C)
1 tube of Fluormone GS1 (store at -5 to -30°C)
2 bottles Buffer (store at -5 to -30°C)
1 tube Tb-Anti-GST Ab (store at -5 to -30°C)
5 tubes GR Stabilizing Peptide (store at -5 to -30°C)
1 tube of DTT (store at -5 to -30°C)