CytoTune™ EmGFP Sendai Fluorescence Reporter

Numéro de catalogue:  A16519

Invitrogen™  Applications liées: Stem Cell Engineering & Reprogramming

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Description

The CytoTune™ EmGFP Sendai Fluorescence Reporter helps you determine whether the Sendai particles that are used in all CytoTune™-iPS Sendai reprogramming kits can transduce into a given cell type before you advance to one of these kits. This vector expresses the next-generation Aequorea victoria EGFP gene, encoding the emerald green fluorescent protein (EmGFP). The cell will express the EmGFP, which is bright, photostable, and ideal for testing transduction.

The CytoTune™ EmGFP Sendai Fluorescence Reporter enables a better understanding of how both the original CytoTune™-iPS Sendai Reprogramming kits and the newer CytoTune™-iPS 2.0 Sendai Reprogramming Kit transduces various cell types. The Sendai particle enters a cell via its HN envelope protein that recognizes sialic acid and is widely expressed in mammalian cells, allowing Sendai virus to target a wide range of cell types. The Sendai virus vectors do not enter the nucleus, thus avoiding any DNA phase, as the viral genome remains as RNA in the cytoplasm.
For Research Use Only. Not for use in diagnostic procedures.

Spécifications

Delivery Method: Sendai virus
Product Line: CytoTune™
Product Size: 1 pack
Promoter: Proprietary
Reporter: GFP (EmGFP)
Sub-Cellular Localization: Cytoplasm

Contenu et stockage

1 tube, store at -68 to -85°C.

Documents

Manuels et protocoles

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