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Thermo Scientific Chemicals
Cresol Red
CAS: 1733-12-6 | C21H18O5S | 382.43 g/mol
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Catalog number A17243.14
also known as A17243-14
Price (TWD)
-
Quantity:
25 g
Chemical Identifiers
CAS1733-12-6
IUPAC Name3,3-bis(4-hydroxy-3-methylphenyl)-3H-2,1λ⁶-benzoxathiole-1,1-dione
Molecular FormulaC21H18O5S
InChI KeyOBRMNDMBJQTZHV-UHFFFAOYSA-N
SMILESCC1=CC(=CC=C1O)C1(OS(=O)(=O)C2=CC=CC=C12)C1=CC=C(O)C(C)=C1
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Specifications
Specification Sheet
Specification SheetAppearance (Color)Dark green or red-brown or dark brown
FormPowder
Color ChangepH 0.2-1.8: orange to yellow
Color ChangepH 7.0-8.8: yellow to red/purple
Loss on Drying≤3.5% (105°C, constant weight)
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Cresol red is commonly used with lead acetate for the detection of urease after PAGE. It is also used as an indicator with the following transition intervals: pH 1.8 (orange) to pH 2.0 (yellow); pH 7.0 (yellow) to pH 8.8 (violet). Cresol Red is used as a tracking dye in DNA, RNA (agarose) and protein (polyacrylamide) electrophoresis. In agarose, Cresol Red runs with an apparent molecular size of approx. 125 bp DNA. Cresol Red does not inhibit Taq polymerase.
This Thermo Scientific Chemicals brand product was originally part of the Alfa Aesar product portfolio. Some documentation and label information may refer to the legacy brand. The original Alfa Aesar product / item code or SKU reference has not changed as a part of the brand transition to Thermo Scientific Chemicals.
Applications
Cresol red is commonly used with lead acetate for the detection of urease after PAGE. It is also used as an indicator with the following transition intervals: pH 1.8 (orange) to pH 2.0 (yellow); pH 7.0 (yellow) to pH 8.8 (violet). Cresol Red is used as a tracking dye in DNA, RNA (agarose) and protein (polyacrylamide) electrophoresis. In agarose, Cresol Red runs with an apparent molecular size of approx. 125 bp DNA. Cresol Red does not inhibit Taq polymerase.
Solubility
Soluble in water (slightly at 20°C), 50% ethanol (0.1%), dil. acids, and dil. alkalis. Insoluble in benzene.
Notes
Transition interval (acid): pH 1.8 (orange) to pH 2.0 (yellow); (alkaline) pH 7.0 (yellow) to pH 8.8 (violet)
Cresol red is commonly used with lead acetate for the detection of urease after PAGE. It is also used as an indicator with the following transition intervals: pH 1.8 (orange) to pH 2.0 (yellow); pH 7.0 (yellow) to pH 8.8 (violet). Cresol Red is used as a tracking dye in DNA, RNA (agarose) and protein (polyacrylamide) electrophoresis. In agarose, Cresol Red runs with an apparent molecular size of approx. 125 bp DNA. Cresol Red does not inhibit Taq polymerase.
Solubility
Soluble in water (slightly at 20°C), 50% ethanol (0.1%), dil. acids, and dil. alkalis. Insoluble in benzene.
Notes
Transition interval (acid): pH 1.8 (orange) to pH 2.0 (yellow); (alkaline) pH 7.0 (yellow) to pH 8.8 (violet)
RUO – Research Use Only
General References:
- Robert H. Byrne.; Jabe A. Breland. High precision multiwavelength pH determinations in seawater using cresol red. Deep Sea Research Part A. Oceanographic Research Papers. 1989, 36 (5), 803-810.
- M. Neus Piña.; M. Carmen Rotger.; Antoni Costa.; Pablo Ballester.; Evaluation of anion selectivity in protic media by squaramide-Cresol Red ensembles. Pere M. Deyà. Tetrahedron Lett. 2004, 45 (19),3749-3752.
- Acid-base indicator: pH 0.2 - 1.8; 7.0 - 8.8.