Gibco™

Human Episomal iPSC Line

Catalog number: A18945
Gibco™

Human Episomal iPSC Line

Catalog number: A18945
Catalog Number
A18945
Unit Size
1 x 106 cells⁄vial
Price (USD)
1,244.00
Your Price:
Availability
***
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
A189451 x 106 cells⁄vial
1,244.00
Your Price:
***
Product Overview
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The Gibco Human Episomal iPSC Line was derived from CD34+ cord blood using a three-plasmid, seven-factor (SOKMNLT; SOX2, OCT4 (POU5F1), KLF4, MYC, NANOG, LIN28, and SV40L T antigen) EBNA-based episomal system. This iPSC line is considered to be zero foot-print as there was no integration into the genome from the reprogramming event.It has been shown to be free of all reprogramming genes and will provide researchers with a control line to compare and study a multitude of aspects of stem cell research.

The Gibco Human Episomal iPSC Line has a normal karyotype and endogenous expression of pluripotent markers like Oct4, Sox2, and Nanog (as shown by RT-PCR) and Oct4, SSEA4, TRA-1-60 and TRA-1-81 (as shown by ICC). Global gene expression analyses demonstrated that this episomal hiPSC line is molecularly indistinguishable from human embryonic stem cell lines (2). In directed differentiation and teratoma analyses, these hiPSCs retained their differentiation potential for the ectodermal, endodermal, and mesodermal lineages (1). In addition, vascular, hematopoietic, neural, and cardiac lineages were derived with robust efficiencies (1).

References
1. Burridge PW, Thompson S, Millrod MA, et al. 2011. A universal system for highly efficient cardiac differentiation of human induced pluripotent stem cells that eliminates interline variability. PLoS One 6(4): e18293.
2. Quintanilla RH Jr, Asprer JST, Vaz C, et al. 2014. CD44 Is a Negative Cell Surface Marker for Pluripotent Stem Cell Identification during Human Fibroblast Reprogramming. PLoS One 9(1): e85419.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Classification
Xeno-Free
Media Recommendation
Essential 8 Flex, StemFlex
No. of Cells
1 x 106
Species
Human
Culture Type
Feeder Cell-Free
Cell Type
Stem Cells (iPS - Induced Pluripotent Stem)
Quantity
1 vial(s)

Contents & Storage

1 vial cells, store in liquid nitrogen (vapor phase).

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