Alexa Fluor™ Antibody Labeling Kits
Alexa Fluor™ Antibody Labeling Kits
Invitrogen™

Alexa Fluor™ Antibody Labeling Kits

Multiplex with brighter, photostable, Alexa Fluor Antibody Labeling Kits without the intramolecular quenching. Conjugate antibodies to 9 available fluorophores for flow cytometry, ICC, IHC, IF, ELISA or HCS. Each kit contains enough dye to conjugate 100 μg per reaction with 5 reactions per kit.
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Catalog NumberExcitation/EmissionColor
A20181494/521 nmGreen
A20182
also known as A-20182
531/554 nmYellow
A20183558/573 nmOrange
A20187553/566 nmOrange
A20184579/603 nmOrange-red
A20185590/616 nmRed
A20186650/668 nmFar-red
A20188680/700 nmNear-IR
A20189784/814 nmNear-IR
Catalog number A20181
Price (USD)/ 1 kit
655.65
Online exclusive
684.00 
Save 28.35 (4%)
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Add to cart
Excitation/Emission:
494/521 nm
Color:
Green
Price (USD)/ 1 kit
655.65
Online exclusive
684.00 
Save 28.35 (4%)
Add to cart
Alexa Fluor™ Antibody Labeling Kits
Catalog numberA20181
Price (USD)/ 1 kit
655.65
Online exclusive
684.00 
Save 28.35 (4%)
-
Add to cart

Alexa Fluor antibody labeling kits provide a convenient means to label antibodies or proteins (>40 kDa) with as many as nine different colored dyes. Conjugated antibodies produce lower background fluorescence with less nonspecific binding and are ideal for multiplexing in a variety of applications. Each kit contains sufficient dye to conjugate 100 μg per reaction and enough reagent for five reactions. In as little as 90 minutes, your samples can be conjugated and ready to use.

Using the fluorescent dyes provided in our Alexa Fluor antibody labeling kits allows multiple primary antibodies of the same isotype, or antibodies derived from the same species, to be used in the same experiment by directly labeling them with compatible fluorophores. Conjugates are ideal for multiple applications including flow cytometry, fluorescent microscopy techniques, immunocytochemistry, immunohistochemistry, primary detection, ELISA, indirect FISH, and more. Each kit contains all required reagents and fluorophores to perform five separate labeling reactions as well as to purify the resulting conjugates. These kits are compatible with each other, easy to use, and make multiplexing a reality in various applications requiring imaging.

Features of Alexa Fluor antibody labeling kits include:
• Labeled proteins ready to use in 90 min. (∼15 min. hands-on time)
• Label up to 100 μg of protein per reaction (five reactions per kit)
• Label proteins greater than 40 kDa
• Includes convenient spin filters
• Stabilizing proteins such as BSA should be removed from the sample before labeling
• Includes detailed instructions for determining degree of labeling (DOL)

The different antibody labeling kits
Green-fluorescent Alexa Fluor 488—excitation and emission maxima of 494/521 nm; excited using a 488-nm argon laser line and detected under standard FITC/Cy2 filters
Yellow-fluorescent Alexa Fluor 532 —excitation and emission maxima of 531/554 nm; excited using a 532-nm Nd:YAG laser line and detected under standard rhodamine 6G filters
Orange-fluorescent Alexa Fluor 546—excitation and emission maxima of 558/573 nm; excited using a 543-nm He-Ne laser line and detected under standard TRITC/Cy3 filters
Orange-fluorescent Alexa Fluor 555—excitation and emission maxima of 553/566 nm; excited using a 543-nm He-Ne laser line and detected under standard TRITC/Cy3 filters
Orange-red-fluorescent Alexa Fluor 568—excitation and emission maxima of 579/603 nm; excited using a 568-nm Kr laser line and detected under standard Rhodamine Red/Cy3.5 filters
Red-fluorescent Alexa Fluor 594—excitation and emission maxima of 590/616 nm, excited using a 594-nm Kr or He-Ne laser line and detected under standard Texas Red filters
Far-red-fluorescent Alexa Fluor 647—excitation and emission maxima of 650/668 nm, excited using a 633- or 635-nm Kr or He-Ne laser line and detected under standard APC/Cy5 filters
Near-IR-fluorescent Alexa Fluor 680—excitation and emission maxima of 680/700 nm
Near-IR-fluorescent Alexa Fluor 790—excitation and emission maxima of 784/814 nm

Compared to traditional dyes, Alexa Fluor dyes are brighter, more photostable, and more pH resistant between pH 4 and 10. When using Alexa Fluor dyes, higher degrees of labeling can be achieved without intramolecular quenching. Our custom conjugation service is efficient, confidential, and ISO 13485:2000 certified. We stand behind the quality of our work.

For Research Use Only. Not for use in diagnostic procedures.
Specifications
Product TypeLabeling Kit
Detection MethodFluorescence
Excitation/Emission494/521 nm
Labeling MethodConjugation-based
Labeling Scale100 μg
Shipping ConditionRoom Temperature
Product LineAlexa Fluor™
Chemical ReactivityAmine
SolubilityDMSO (Dimethylsulfoxide)
Label or DyeAlexa Fluor 488
ColorGreen
Labeling TargetAntibodies, Proteins
Label TypeAlexa Fluor
Unit Size1 kit
Contents & Storage
Alexa Fluor™ 488 Antibody Labeling Kit comes with 5 vials of reactive dye, sodium bicarbonate, purification resin, spin columns, and collections tubes.

Store at 2°C to 8°C, protected from light. Component A may be stored from -20°C to +8°C.