Invitrogen™

Alexa Fluor™ Antibody Labeling Kits

Catalog number: A20182
Invitrogen™

Alexa Fluor™ Antibody Labeling Kits

Catalog number: A20182
Alexa Fluor antibody labeling kits provide a convenient means to label antibodies or proteins (>40 kDa) with as many as nine different colored dyes. Conjugated antibodies produce lower background fluorescence with less nonspecific binding and are ideal for multiplexing in a variety of applications. Each kit contains sufficient dye to conjugate 100 μg per reaction and enough reagent for five reactions. In as little as 90 minutes, your samples can be conjugated and ready to use.
 
Catalog Number
A20182
also known as A-20182
Unit Size
1 kit
Description
Alexa Fluor™ 532 Antibody Labeling Kit
Excitation/Emission
531/554 nm
Color
Yellow
Price (USD)
Price: 658.00
Online price: 622.65
Your price:
Availability
-
Full specifications
Quantity
DescriptionAlexa Fluor™ 532 Antibody Labeling Kit
Product TypeLabeling Kit
Detection MethodFluorescence
Excitation/Emission531/554 nm
Labeling MethodConjugation-based
Labeling Scale100 μg
Shipping ConditionRoom Temperature
Product LineAlexa Fluor™
Chemical ReactivityAmine
SolubilityDMSO (Dimethylsulfoxide)
Label or DyeAlexa Fluor 532
ColorYellow
Labeling TargetAntibodies, Proteins
Label TypeAlexa Fluor
Unit Size1 kit
Catalog Number
Unit Size
1 kit
Description
Alexa Fluor™ 488 Antibody Labeling Kit
Excitation/Emission
494/521 nm
Color
Green
Price (USD)
Price: 684.00
Online price: 655.65
Your price:
Availability
-
Full specifications
Quantity
DescriptionAlexa Fluor™ 488 Antibody Labeling Kit
Product TypeLabeling Kit
Detection MethodFluorescence
Excitation/Emission494/521 nm
Labeling MethodConjugation-based
Labeling Scale100 μg
Shipping ConditionRoom Temperature
Product LineAlexa Fluor™
Chemical ReactivityAmine
SolubilityDMSO (Dimethylsulfoxide)
Label or DyeAlexa Fluor 488
ColorGreen
Labeling TargetAntibodies, Proteins
Label TypeAlexa Fluor
Unit Size1 kit
Catalog Number
A20183
also known as A-20183
Unit Size
1 kit
Description
Alexa Fluor™ 546 Antibody Labeling Kit
Excitation/Emission
558/573 nm
Color
Orange
Price (USD)
Price: 664.00
Online price: 628.65
Your price:
Availability
-
Full specifications
Quantity
DescriptionAlexa Fluor™ 546 Antibody Labeling Kit
Product TypeLabeling Kit
Detection MethodFluorescence
Excitation/Emission558/573 nm
Labeling MethodConjugation-based
Labeling Scale100 μg
Shipping ConditionRoom Temperature
Product LineAlexa Fluor™
Chemical ReactivityAmine
SolubilityDMSO (Dimethylsulfoxide)
Label or DyeAlexa Fluor 546
ColorOrange
Labeling TargetAntibodies, Proteins
Label TypeAlexa Fluor
Unit Size1 kit
Catalog Number
Unit Size
1 kit
Description
Alexa Fluor™ 555 Antibody Labeling Kit
Excitation/Emission
553/566 nm
Color
Orange
Price (USD)
Price: 684.00
Online price: 647.65
Your price:
Availability
-
Full specifications
Quantity
DescriptionAlexa Fluor™ 555 Antibody Labeling Kit
Product TypeLabeling Kit
Detection MethodFluorescence
Excitation/Emission553/566 nm
Labeling MethodConjugation-based
Labeling Scale100 μg
Shipping ConditionRoom Temperature
Product LineAlexa Fluor™
Chemical ReactivityAmine
SolubilityDMSO (Dimethylsulfoxide)
Label or DyeAlexa Fluor 555
ColorOrange
Labeling TargetAntibodies, Proteins
Label TypeAlexa Fluor
Unit Size1 kit
Catalog Number
A20184
also known as A-20184
Unit Size
1 kit
Description
Alexa Fluor™ 568 Antibody Labeling Kit
Excitation/Emission
579/603 nm
Color
Orange-red
Price (USD)
Price: 672.00
Online price: 635.65
Your price:
Availability
-
Full specifications
Quantity
DescriptionAlexa Fluor™ 568 Antibody Labeling Kit
Product TypeLabeling Kit
Detection MethodFluorescence
Excitation/Emission579/603 nm
Labeling MethodConjugation-based
Labeling Scale100 μg
Shipping ConditionRoom Temperature
Product LineAlexa Fluor™
Chemical ReactivityAmine
SolubilityDMSO (Dimethylsulfoxide)
Label or DyeAlexa Fluor 568
ColorOrange-red
Labeling TargetAntibodies, Proteins
Label TypeAlexa Fluor
Unit Size1 kit
Catalog Number
A20185
also known as A-20185
Unit Size
1 kit
Description
Alexa Fluor™ 594 Antibody Labeling Kit
Excitation/Emission
590/616 nm
Color
Red
Price (USD)
Price: 672.00
Online price: 635.65
Your price:
Availability
-
Full specifications
Quantity
DescriptionAlexa Fluor™ 594 Antibody Labeling Kit
Product TypeLabeling Kit
Detection MethodFluorescence
Excitation/Emission590/616 nm
Labeling MethodConjugation-based
Labeling Scale100 μg
Shipping ConditionRoom Temperature
Product LineAlexa Fluor™
Chemical ReactivityAmine
SolubilityDMSO (Dimethylsulfoxide)
Label or DyeAlexa Fluor 594
ColorRed
Labeling TargetAntibodies, Proteins
Label TypeAlexa Fluor
Unit Size1 kit
Catalog Number
A20186
also known as A-20186
Unit Size
1 kit
Description
Alexa Fluor™ 647 Antibody Labeling Kit
Excitation/Emission
650/668 nm
Color
Far-red
Price (USD)
Price: 674.00
Online price: 637.65
Your price:
Availability
-
Full specifications
Quantity
DescriptionAlexa Fluor™ 647 Antibody Labeling Kit
Product TypeLabeling Kit
Detection MethodFluorescence
Excitation/Emission650/668 nm
Labeling MethodConjugation-based
Labeling Scale100 μg
Shipping ConditionRoom Temperature
Product LineAlexa Fluor™
Chemical ReactivityAmine
SolubilityDMSO (Dimethylsulfoxide)
Label or DyeAlexa Fluor 647
ColorFar-red
Labeling TargetAntibodies, Proteins
Label TypeAlexa Fluor
Unit Size1 kit
Catalog Number
Unit Size
1 kit
Description
Alexa Fluor™ 680 Antibody Labeling Kit
Excitation/Emission
680/700 nm
Color
Near-IR
Price (USD)
Price: 648.00
Online price: 613.65
Your price:
Availability
-
Full specifications
Quantity
DescriptionAlexa Fluor™ 680 Antibody Labeling Kit
Product TypeLabeling Kit
Quantity5 Reactions
Detection MethodFluorescence
Excitation/Emission680/700 nm
For Use With (Equipment)Fluorescent Imager
Labeling MethodConjugation-based
Labeling Scale100 μg
Shipping ConditionRoom Temperature
Product LineAlexa Fluor™
Chemical ReactivityAmine
SolubilityDMSO (Dimethylsulfoxide)
Label or DyeAlexa Fluor 680
ColorNear-IR
Labeling TargetAntibodies
Label TypeAlexa Fluor
Unit Size1 kit
Catalog Number
Unit Size
1 kit
Description
Alexa Fluor™ 790 Antibody Labeling Kit
Excitation/Emission
784/814 nm
Color
Near-IR
Price (USD)
Price: 632.00
Online price: 598.65
Your price:
Availability
-
Full specifications
Quantity
DescriptionAlexa Fluor™ 790 Antibody Labeling Kit
Product TypeLabeling Kit
Quantity5 Reactions
Detection MethodFluorescence
Excitation/Emission784/814 nm
For Use With (Equipment)Fluorescent Imager
Labeling MethodConjugation-based
Labeling Scale100 μg
Shipping ConditionRoom Temperature
Product LineAlexa Fluor™
Chemical ReactivityAmine
SolubilityDMSO (Dimethylsulfoxide)
Label or DyeAlexa Fluor 790
ColorNear-IR
Labeling TargetAntibodies
Label TypeAlexa Fluor
Unit Size1 kit
Showing 9 of 9
Catalog NumberSpecificationsUnit SizeDescriptionExcitation/EmissionColorPrice (USD)AvailabilityQuantity
A20182
also known as A-20182
Full specifications
1 kitAlexa Fluor™ 532 Antibody Labeling Kit531/554 nmYellow
Price: 658.00
Online price: 622.65
Your price:
-
DescriptionAlexa Fluor™ 532 Antibody Labeling Kit
Product TypeLabeling Kit
Detection MethodFluorescence
Excitation/Emission531/554 nm
Labeling MethodConjugation-based
Labeling Scale100 μg
Shipping ConditionRoom Temperature
Product LineAlexa Fluor™
Chemical ReactivityAmine
SolubilityDMSO (Dimethylsulfoxide)
Label or DyeAlexa Fluor 532
ColorYellow
Labeling TargetAntibodies, Proteins
Label TypeAlexa Fluor
Unit Size1 kit
A20181Full specifications
1 kitAlexa Fluor™ 488 Antibody Labeling Kit494/521 nmGreen
Price: 684.00
Online price: 655.65
Your price:
-
DescriptionAlexa Fluor™ 488 Antibody Labeling Kit
Product TypeLabeling Kit
Detection MethodFluorescence
Excitation/Emission494/521 nm
Labeling MethodConjugation-based
Labeling Scale100 μg
Shipping ConditionRoom Temperature
Product LineAlexa Fluor™
Chemical ReactivityAmine
SolubilityDMSO (Dimethylsulfoxide)
Label or DyeAlexa Fluor 488
ColorGreen
Labeling TargetAntibodies, Proteins
Label TypeAlexa Fluor
Unit Size1 kit
A20183
also known as A-20183
Full specifications
1 kitAlexa Fluor™ 546 Antibody Labeling Kit558/573 nmOrange
Price: 664.00
Online price: 628.65
Your price:
-
DescriptionAlexa Fluor™ 546 Antibody Labeling Kit
Product TypeLabeling Kit
Detection MethodFluorescence
Excitation/Emission558/573 nm
Labeling MethodConjugation-based
Labeling Scale100 μg
Shipping ConditionRoom Temperature
Product LineAlexa Fluor™
Chemical ReactivityAmine
SolubilityDMSO (Dimethylsulfoxide)
Label or DyeAlexa Fluor 546
ColorOrange
Labeling TargetAntibodies, Proteins
Label TypeAlexa Fluor
Unit Size1 kit
A20187Full specifications
1 kitAlexa Fluor™ 555 Antibody Labeling Kit553/566 nmOrange
Price: 684.00
Online price: 647.65
Your price:
-
DescriptionAlexa Fluor™ 555 Antibody Labeling Kit
Product TypeLabeling Kit
Detection MethodFluorescence
Excitation/Emission553/566 nm
Labeling MethodConjugation-based
Labeling Scale100 μg
Shipping ConditionRoom Temperature
Product LineAlexa Fluor™
Chemical ReactivityAmine
SolubilityDMSO (Dimethylsulfoxide)
Label or DyeAlexa Fluor 555
ColorOrange
Labeling TargetAntibodies, Proteins
Label TypeAlexa Fluor
Unit Size1 kit
A20184
also known as A-20184
Full specifications
1 kitAlexa Fluor™ 568 Antibody Labeling Kit579/603 nmOrange-red
Price: 672.00
Online price: 635.65
Your price:
-
DescriptionAlexa Fluor™ 568 Antibody Labeling Kit
Product TypeLabeling Kit
Detection MethodFluorescence
Excitation/Emission579/603 nm
Labeling MethodConjugation-based
Labeling Scale100 μg
Shipping ConditionRoom Temperature
Product LineAlexa Fluor™
Chemical ReactivityAmine
SolubilityDMSO (Dimethylsulfoxide)
Label or DyeAlexa Fluor 568
ColorOrange-red
Labeling TargetAntibodies, Proteins
Label TypeAlexa Fluor
Unit Size1 kit
A20185
also known as A-20185
Full specifications
1 kitAlexa Fluor™ 594 Antibody Labeling Kit590/616 nmRed
Price: 672.00
Online price: 635.65
Your price:
-
DescriptionAlexa Fluor™ 594 Antibody Labeling Kit
Product TypeLabeling Kit
Detection MethodFluorescence
Excitation/Emission590/616 nm
Labeling MethodConjugation-based
Labeling Scale100 μg
Shipping ConditionRoom Temperature
Product LineAlexa Fluor™
Chemical ReactivityAmine
SolubilityDMSO (Dimethylsulfoxide)
Label or DyeAlexa Fluor 594
ColorRed
Labeling TargetAntibodies, Proteins
Label TypeAlexa Fluor
Unit Size1 kit
A20186
also known as A-20186
Full specifications
1 kitAlexa Fluor™ 647 Antibody Labeling Kit650/668 nmFar-red
Price: 674.00
Online price: 637.65
Your price:
-
DescriptionAlexa Fluor™ 647 Antibody Labeling Kit
Product TypeLabeling Kit
Detection MethodFluorescence
Excitation/Emission650/668 nm
Labeling MethodConjugation-based
Labeling Scale100 μg
Shipping ConditionRoom Temperature
Product LineAlexa Fluor™
Chemical ReactivityAmine
SolubilityDMSO (Dimethylsulfoxide)
Label or DyeAlexa Fluor 647
ColorFar-red
Labeling TargetAntibodies, Proteins
Label TypeAlexa Fluor
Unit Size1 kit
A20188Full specifications
1 kitAlexa Fluor™ 680 Antibody Labeling Kit680/700 nmNear-IR
Price: 648.00
Online price: 613.65
Your price:
-
DescriptionAlexa Fluor™ 680 Antibody Labeling Kit
Product TypeLabeling Kit
Quantity5 Reactions
Detection MethodFluorescence
Excitation/Emission680/700 nm
For Use With (Equipment)Fluorescent Imager
Labeling MethodConjugation-based
Labeling Scale100 μg
Shipping ConditionRoom Temperature
Product LineAlexa Fluor™
Chemical ReactivityAmine
SolubilityDMSO (Dimethylsulfoxide)
Label or DyeAlexa Fluor 680
ColorNear-IR
Labeling TargetAntibodies
Label TypeAlexa Fluor
Unit Size1 kit
A20189Full specifications
1 kitAlexa Fluor™ 790 Antibody Labeling Kit784/814 nmNear-IR
Price: 632.00
Online price: 598.65
Your price:
-
DescriptionAlexa Fluor™ 790 Antibody Labeling Kit
Product TypeLabeling Kit
Quantity5 Reactions
Detection MethodFluorescence
Excitation/Emission784/814 nm
For Use With (Equipment)Fluorescent Imager
Labeling MethodConjugation-based
Labeling Scale100 μg
Shipping ConditionRoom Temperature
Product LineAlexa Fluor™
Chemical ReactivityAmine
SolubilityDMSO (Dimethylsulfoxide)
Label or DyeAlexa Fluor 790
ColorNear-IR
Labeling TargetAntibodies
Label TypeAlexa Fluor
Unit Size1 kit
Showing 9 of 9

Using the fluorescent dyes provided in our Alexa Fluor antibody labeling kits allows multiple primary antibodies of the same isotype, or antibodies derived from the same species, to be used in the same experiment by directly labeling them with compatible fluorophores. Conjugates are ideal for multiple applications including flow cytometry, fluorescent microscopy techniques, immunocytochemistry, immunohistochemistry, primary detection, ELISA, indirect FISH, and more. Each kit contains all required reagents and fluorophores to perform five separate labeling reactions as well as to purify the resulting conjugates. These kits are compatible with each other, easy to use, and make multiplexing a reality in various applications requiring imaging.

Features of Alexa Fluor antibody labeling kits include:
• Labeled proteins ready to use in 90 min. (∼15 min. hands-on time)
• Label up to 100 μg of protein per reaction (five reactions per kit)
• Label proteins greater than 40 kDa
• Includes convenient spin filters
• Stabilizing proteins such as BSA should be removed from the sample before labeling
• Includes detailed instructions for determining degree of labeling (DOL)

The different antibody labeling kits
Green-fluorescent Alexa Fluor 488—excitation and emission maxima of 494/521 nm; excited using a 488-nm argon laser line and detected under standard FITC/Cy2 filters
Yellow-fluorescent Alexa Fluor 532 —excitation and emission maxima of 531/554 nm; excited using a 532-nm Nd:YAG laser line and detected under standard rhodamine 6G filters
Orange-fluorescent Alexa Fluor 546—excitation and emission maxima of 558/573 nm; excited using a 543-nm He-Ne laser line and detected under standard TRITC/Cy3 filters
Orange-fluorescent Alexa Fluor 555—excitation and emission maxima of 553/566 nm; excited using a 543-nm He-Ne laser line and detected under standard TRITC/Cy3 filters
Orange-red-fluorescent Alexa Fluor 568—excitation and emission maxima of 579/603 nm; excited using a 568-nm Kr laser line and detected under standard Rhodamine Red/Cy3.5 filters
Red-fluorescent Alexa Fluor 594—excitation and emission maxima of 590/616 nm, excited using a 594-nm Kr or He-Ne laser line and detected under standard Texas Red filters
Far-red-fluorescent Alexa Fluor 647—excitation and emission maxima of 650/668 nm, excited using a 633- or 635-nm Kr or He-Ne laser line and detected under standard APC/Cy5 filters
Near-IR-fluorescent Alexa Fluor 680—excitation and emission maxima of 680/700 nm
Near-IR-fluorescent Alexa Fluor 790—excitation and emission maxima of 784/814 nm

Compared to traditional dyes, Alexa Fluor dyes are brighter, more photostable, and more pH resistant between pH 4 and 10. When using Alexa Fluor dyes, higher degrees of labeling can be achieved without intramolecular quenching. Our custom conjugation service is efficient, confidential, and ISO 13485:2000 certified. We stand behind the quality of our work.

For Research Use Only. Not for use in diagnostic procedures.

Contents & Storage

Alexa Fluor™ 532 Antibody Labeling Kit comes with 5 vials of reactive dye, sodium bicarbonate, purification resin, spin columns, and collections tubes.

Store at 2°C to 8°C, protected from light. Component A may be stored from -20°C to +8°C.

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