Invitrogen™

Alexa Fluor™ Antibody Labeling Kits

Catalog number: A20185
Invitrogen™

Alexa Fluor™ Antibody Labeling Kits

Catalog number: A20185
Alexa Fluor antibody labeling kits provide a convenient means to label antibodies or proteins (>40 kDa) with as many as nine different colored dyes. Conjugated antibodies produce lower background fluorescence with less nonspecific binding and are ideal for multiplexing in a variety of applications. Each kit contains sufficient dye to conjugate 100 µg per reaction and enough reagent for five reactions. In as little as 90 minutes, your samples can be conjugated and ready to use.
 
Catalog Number
A20185
Unit Size
1 kit
Description
Alexa Fluor™ 594 Antibody Labeling Kit
Excitation/Emission
590/616
Color
Red
Price (USD)
Price: 620.00
Your Price:
Availability
-
Full specifications
Quantity
DescriptionAlexa Fluor™ 594 Antibody Labeling Kit
Product TypeAntibody Labeling Kit
Detection MethodFluorescence
Excitation/Emission590/616
Labeling MethodConjugation-Based
Labeling Scale100 μg
Shipping ConditionRoom Temperature
Product LineAlexa Fluor™
Chemical ReactivityAmine
SolubilityDMSO (Dimethylsulfoxide)
Label or DyeAlexa Fluor™ 594
Label TypeAlexa Fluor Dyes
ColorRed
Labeling TargetAntibodies (General), Proteins (General)
Unit Size1 kit
Catalog Number
Unit Size
1 kit
Description
Alexa Fluor™ 488 Antibody Labeling Kit
Excitation/Emission
494/521
Color
Green
Price (USD)
Price: 630.00
Your Price:
Availability
-
Full specifications
Quantity
DescriptionAlexa Fluor™ 488 Antibody Labeling Kit
Product TypeAntibody Labeling Kit
Detection MethodFluorescence
Excitation/Emission494/521
Labeling MethodConjugation-Based
Labeling Scale100 μg
Shipping ConditionRoom Temperature
Product LineAlexa Fluor™
Chemical ReactivityAmine
SolubilityDMSO (Dimethylsulfoxide)
Label or DyeAlexa Fluor™ 488
Label TypeAlexa Fluor Dyes
ColorGreen
Labeling TargetAntibodies (General), Proteins (General)
Unit Size1 kit
Catalog Number
A20182
also known as A-20182
Unit Size
1 kit
Description
Alexa Fluor™ 532 Antibody Labeling Kit
Excitation/Emission
531/554
Color
Yellow
Price (USD)
Price: 606.00
Your Price:
Availability
-
Full specifications
Quantity
DescriptionAlexa Fluor™ 532 Antibody Labeling Kit
Product TypeAntibody Labeling Kit
Detection MethodFluorescence
Excitation/Emission531/554
Labeling MethodConjugation-Based
Labeling Scale100 μg
Shipping ConditionRoom Temperature
Product LineAlexa Fluor™
Chemical ReactivityAmine
SolubilityDMSO (Dimethylsulfoxide)
Label or DyeAlexa Fluor™ 532
Label TypeAlexa Fluor Dyes
ColorYellow
Labeling TargetAntibodies (General), Proteins (General)
Unit Size1 kit
Catalog Number
Unit Size
1 kit
Description
Alexa Fluor™ 546 Antibody Labeling Kit
Excitation/Emission
558/573
Color
Orange
Price (USD)
Price: 612.00
Your Price:
Availability
-
Full specifications
Quantity
DescriptionAlexa Fluor™ 546 Antibody Labeling Kit
Product TypeAntibody Labeling Kit
Detection MethodFluorescence
Excitation/Emission558/573
Labeling MethodConjugation-Based
Labeling Scale100 μg
Shipping ConditionRoom Temperature
Product LineAlexa Fluor™
Chemical ReactivityAmine
SolubilityDMSO (Dimethylsulfoxide)
Label or DyeAlexa Fluor™ 546
Label TypeAlexa Fluor Dyes
ColorOrange
Labeling TargetAntibodies (General), Proteins (General)
Unit Size1 kit
Catalog Number
Unit Size
1 kit
Description
Alexa Fluor™ 555 Antibody Labeling Kit
Excitation/Emission
553/566
Color
Orange
Price (USD)
Price: 630.00
Your Price:
Availability
-
Full specifications
Quantity
DescriptionAlexa Fluor™ 555 Antibody Labeling Kit
Product TypeAntibody Labeling Kit
Detection MethodFluorescence
Excitation/Emission553/566
Labeling MethodConjugation-Based
Labeling Scale100 μg
Shipping ConditionRoom Temperature
Product LineAlexa Fluor™
Chemical ReactivityAmine
SolubilityDMSO (Dimethylsulfoxide)
Label or DyeAlexa Fluor™ 555
Label TypeAlexa Fluor Dyes
ColorOrange
Labeling TargetAntibodies (General), Proteins (General)
Unit Size1 kit
Catalog Number
Unit Size
1 kit
Description
Alexa Fluor™ 568 Antibody Labeling Kit
Excitation/Emission
579/603
Color
Orange-red
Price (USD)
Price: 620.00
Your Price:
Availability
-
Full specifications
Quantity
DescriptionAlexa Fluor™ 568 Antibody Labeling Kit
Product TypeAntibody Labeling Kit
Detection MethodFluorescence
Excitation/Emission579/603
Labeling MethodConjugation-Based
Labeling Scale100 μg
Shipping ConditionRoom Temperature
Product LineAlexa Fluor™
Chemical ReactivityAmine
SolubilityDMSO (Dimethylsulfoxide)
Label or DyeAlexa Fluor™ 568
Label TypeAlexa Fluor Dyes
ColorOrange-red
Labeling TargetAntibodies (General), Proteins (General)
Unit Size1 kit
Catalog Number
Unit Size
1 kit
Description
Alexa Fluor™ 647 Antibody Labeling Kit
Excitation/Emission
650/668
Color
Far-red
Price (USD)
Price: 626.00
Your Price:
Availability
-
Full specifications
Quantity
DescriptionAlexa Fluor™ 647 Antibody Labeling Kit
Product TypeAntibody Labeling Kit
Detection MethodFluorescence
Excitation/Emission650/668
Labeling MethodConjugation-Based
Labeling Scale100 μg
Shipping ConditionRoom Temperature
Product LineAlexa Fluor™
Chemical ReactivityAmine
SolubilityDMSO (Dimethylsulfoxide)
Label or DyeAlexa Fluor™ 647
Label TypeAlexa Fluor Dyes
ColorFar-red
Labeling TargetAntibodies (General), Proteins (General)
Unit Size1 kit
Catalog Number
Unit Size
1 kit
Description
Alexa Fluor™ 680 Antibody Labeling Kit
Excitation/Emission
680/700
Color
Near-IR
Price (USD)
Price: 598.00
Your Price:
Availability
-
Full specifications
Quantity
DescriptionAlexa Fluor™ 680 Antibody Labeling Kit
Product TypeAntibody Labeling Kit
Quantity5 Reactions
Detection MethodFluorescence
Excitation/Emission680/700
For Use With (Equipment)Fluorescent Imager
Labeling MethodConjugation-Based
Labeling Scale100 μg
Shipping ConditionRoom Temperature
Product LineAlexa Fluor™
Chemical ReactivityAmine
SolubilityDMSO (Dimethylsulfoxide)
Label or DyeAlexa Fluor™ 680
Label TypeAlexa Fluor Dyes
ColorNear-IR
Labeling TargetAntibodies (General)
Unit Size1 kit
Catalog Number
Unit Size
1 kit
Description
Alexa Fluor™ 790 Antibody Labeling Kit
Excitation/Emission
784/814
Color
Near-IR
Price (USD)
Price: 582.00
Your Price:
Availability
-
Full specifications
Quantity
DescriptionAlexa Fluor™ 790 Antibody Labeling Kit
Product TypeAntibody Labeling Kit
Quantity5 Reactions
Detection MethodFluorescence
Excitation/Emission784/814
For Use With (Equipment)Fluorescent Imager
Labeling MethodConjugation-Based
Labeling Scale100 μg
Shipping ConditionRoom Temperature
Product LineAlexa Fluor™
Chemical ReactivityAmine
SolubilityDMSO (Dimethylsulfoxide)
Label or DyeAlexa Fluor™ 790
Label TypeAlexa Fluor Dyes
ColorNear-IR
Labeling TargetAntibodies (General)
Unit Size1 kit
Showing 9 of 9
Catalog NumberSpecificationsUnit SizeDescriptionExcitation/EmissionColorPrice (USD)AvailabilityQuantity
A20185Full specifications
1 kitAlexa Fluor™ 594 Antibody Labeling Kit590/616Red
Price: 620.00
Your Price:
-
DescriptionAlexa Fluor™ 594 Antibody Labeling Kit
Product TypeAntibody Labeling Kit
Detection MethodFluorescence
Excitation/Emission590/616
Labeling MethodConjugation-Based
Labeling Scale100 μg
Shipping ConditionRoom Temperature
Product LineAlexa Fluor™
Chemical ReactivityAmine
SolubilityDMSO (Dimethylsulfoxide)
Label or DyeAlexa Fluor™ 594
Label TypeAlexa Fluor Dyes
ColorRed
Labeling TargetAntibodies (General), Proteins (General)
Unit Size1 kit
A20181Full specifications
1 kitAlexa Fluor™ 488 Antibody Labeling Kit494/521Green
Price: 630.00
Your Price:
-
DescriptionAlexa Fluor™ 488 Antibody Labeling Kit
Product TypeAntibody Labeling Kit
Detection MethodFluorescence
Excitation/Emission494/521
Labeling MethodConjugation-Based
Labeling Scale100 μg
Shipping ConditionRoom Temperature
Product LineAlexa Fluor™
Chemical ReactivityAmine
SolubilityDMSO (Dimethylsulfoxide)
Label or DyeAlexa Fluor™ 488
Label TypeAlexa Fluor Dyes
ColorGreen
Labeling TargetAntibodies (General), Proteins (General)
Unit Size1 kit
A20182
also known as A-20182
Full specifications
1 kitAlexa Fluor™ 532 Antibody Labeling Kit531/554Yellow
Price: 606.00
Your Price:
-
DescriptionAlexa Fluor™ 532 Antibody Labeling Kit
Product TypeAntibody Labeling Kit
Detection MethodFluorescence
Excitation/Emission531/554
Labeling MethodConjugation-Based
Labeling Scale100 μg
Shipping ConditionRoom Temperature
Product LineAlexa Fluor™
Chemical ReactivityAmine
SolubilityDMSO (Dimethylsulfoxide)
Label or DyeAlexa Fluor™ 532
Label TypeAlexa Fluor Dyes
ColorYellow
Labeling TargetAntibodies (General), Proteins (General)
Unit Size1 kit
A20183Full specifications
1 kitAlexa Fluor™ 546 Antibody Labeling Kit558/573Orange
Price: 612.00
Your Price:
-
DescriptionAlexa Fluor™ 546 Antibody Labeling Kit
Product TypeAntibody Labeling Kit
Detection MethodFluorescence
Excitation/Emission558/573
Labeling MethodConjugation-Based
Labeling Scale100 μg
Shipping ConditionRoom Temperature
Product LineAlexa Fluor™
Chemical ReactivityAmine
SolubilityDMSO (Dimethylsulfoxide)
Label or DyeAlexa Fluor™ 546
Label TypeAlexa Fluor Dyes
ColorOrange
Labeling TargetAntibodies (General), Proteins (General)
Unit Size1 kit
A20187Full specifications
1 kitAlexa Fluor™ 555 Antibody Labeling Kit553/566Orange
Price: 630.00
Your Price:
-
DescriptionAlexa Fluor™ 555 Antibody Labeling Kit
Product TypeAntibody Labeling Kit
Detection MethodFluorescence
Excitation/Emission553/566
Labeling MethodConjugation-Based
Labeling Scale100 μg
Shipping ConditionRoom Temperature
Product LineAlexa Fluor™
Chemical ReactivityAmine
SolubilityDMSO (Dimethylsulfoxide)
Label or DyeAlexa Fluor™ 555
Label TypeAlexa Fluor Dyes
ColorOrange
Labeling TargetAntibodies (General), Proteins (General)
Unit Size1 kit
A20184Full specifications
1 kitAlexa Fluor™ 568 Antibody Labeling Kit579/603Orange-red
Price: 620.00
Your Price:
-
DescriptionAlexa Fluor™ 568 Antibody Labeling Kit
Product TypeAntibody Labeling Kit
Detection MethodFluorescence
Excitation/Emission579/603
Labeling MethodConjugation-Based
Labeling Scale100 μg
Shipping ConditionRoom Temperature
Product LineAlexa Fluor™
Chemical ReactivityAmine
SolubilityDMSO (Dimethylsulfoxide)
Label or DyeAlexa Fluor™ 568
Label TypeAlexa Fluor Dyes
ColorOrange-red
Labeling TargetAntibodies (General), Proteins (General)
Unit Size1 kit
A20186Full specifications
1 kitAlexa Fluor™ 647 Antibody Labeling Kit650/668Far-red
Price: 626.00
Your Price:
-
DescriptionAlexa Fluor™ 647 Antibody Labeling Kit
Product TypeAntibody Labeling Kit
Detection MethodFluorescence
Excitation/Emission650/668
Labeling MethodConjugation-Based
Labeling Scale100 μg
Shipping ConditionRoom Temperature
Product LineAlexa Fluor™
Chemical ReactivityAmine
SolubilityDMSO (Dimethylsulfoxide)
Label or DyeAlexa Fluor™ 647
Label TypeAlexa Fluor Dyes
ColorFar-red
Labeling TargetAntibodies (General), Proteins (General)
Unit Size1 kit
A20188Full specifications
1 kitAlexa Fluor™ 680 Antibody Labeling Kit680/700Near-IR
Price: 598.00
Your Price:
-
DescriptionAlexa Fluor™ 680 Antibody Labeling Kit
Product TypeAntibody Labeling Kit
Quantity5 Reactions
Detection MethodFluorescence
Excitation/Emission680/700
For Use With (Equipment)Fluorescent Imager
Labeling MethodConjugation-Based
Labeling Scale100 μg
Shipping ConditionRoom Temperature
Product LineAlexa Fluor™
Chemical ReactivityAmine
SolubilityDMSO (Dimethylsulfoxide)
Label or DyeAlexa Fluor™ 680
Label TypeAlexa Fluor Dyes
ColorNear-IR
Labeling TargetAntibodies (General)
Unit Size1 kit
A20189Full specifications
1 kitAlexa Fluor™ 790 Antibody Labeling Kit784/814Near-IR
Price: 582.00
Your Price:
-
DescriptionAlexa Fluor™ 790 Antibody Labeling Kit
Product TypeAntibody Labeling Kit
Quantity5 Reactions
Detection MethodFluorescence
Excitation/Emission784/814
For Use With (Equipment)Fluorescent Imager
Labeling MethodConjugation-Based
Labeling Scale100 μg
Shipping ConditionRoom Temperature
Product LineAlexa Fluor™
Chemical ReactivityAmine
SolubilityDMSO (Dimethylsulfoxide)
Label or DyeAlexa Fluor™ 790
Label TypeAlexa Fluor Dyes
ColorNear-IR
Labeling TargetAntibodies (General)
Unit Size1 kit
Showing 9 of 9

Using the fluorescent dyes provided in our Alexa Fluor antibody labeling kits allows multiple primary antibodies of the same isotype, or antibodies derived from the same species, to be used in the same experiment by directly labeling them with compatible fluorophores. Conjugates are ideal for multiple applications including flow cytometry, fluorescent microscopy techniques, immunocytochemistry, immunohistochemistry, primary detection, ELISA, indirect FISH, and more. Each kit contains all required reagents and fluorophores to perform five separate labeling reactions as well as to purify the resulting conjugates. These kits are compatible with each other, easy to use, and make multiplexing a reality in various applications requiring imaging.

Features of Alexa Fluor antibody labeling kits include:
• Labeled proteins ready to use in 90 min. (∼15 min. hands-on time)
• Label up to 100 µg of protein per reaction (five reactions per kit)
• Label proteins greater than 40 kDa
• Includes convenient spin filters
• Stabilizing proteins such as BSA should be removed from the sample before labeling
• Includes detailed instructions for determining degree of labeling (DOL)

The different antibody labeling kits
Green-fluorescent Alexa Fluor 488—excitation and emission maxima of 494/521 nm; excited using a 488-nm argon laser line and detected under standard FITC/Cy2 filters
Yellow-fluorescent Alexa Fluor 532 —excitation and emission maxima of 531/554 nm; excited using a 532-nm Nd:YAG laser line and detected under standard rhodamine 6G filters
Orange-fluorescent Alexa Fluor 546—excitation and emission maxima of 558/573 nm; excited using a 543-nm He-Ne laser line and detected under standard TRITC/Cy3 filters
Orange-fluorescent Alexa Fluor 555—excitation and emission maxima of 553/566 nm; excited using a 543-nm He-Ne laser line and detected under standard TRITC/Cy3 filters
Orange-red-fluorescent Alexa Fluor 568—excitation and emission maxima of 579/603 nm; excited using a 568-nm Kr laser line and detected under standard Rhodamine Red/Cy3.5 filters
Red-fluorescent Alexa Fluor 594—excitation and emission maxima of 590/616 nm, excited using a 594-nm Kr or He-Ne laser line and detected under standard Texas Red filters
Far-red-fluorescent Alexa Fluor 647—excitation and emission maxima of 650/668 nm, excited using a 633- or 635-nm Kr or He-Ne laser line and detected under standard APC/Cy5 filters
Near-IR-fluorescent Alexa Fluor 680—excitation and emission maxima of 680/700 nm
Near-IR-fluorescent Alexa Fluor 790—excitation and emission maxima of 784/814 nm

Compared to traditional dyes, Alexa Fluor dyes are brighter, more photostable, and more pH resistant between pH 4 and 10. When using Alexa Fluor dyes, higher degrees of labeling can be achieved without intramolecular quenching. Our custom conjugation service is efficient, confidential, and ISO 9001:2000 certified. We stand behind the quality of our work.

For Research Use Only. Not for use in diagnostic procedures.
For Research Use Only. Not for use in diagnostic procedures.

Contents & Storage

Alexa Fluor™ 594 Antibody Labeling Kit comes with 5 vials of reactive dye, sodium bicarbonate, purification resin, spin columns, and collections tubes.

Store at 2°C to 8°C, protected from light. Component A may be stored from -20°C to +8°C.