Aminoallyl dUTP (5-(3-aminoallyl)-2´-deoxyuridine 5´-triphosphate, trisodium Salt), 2 mM in TE buffer

Catalog number: A21664

Molecular Probes™  Related applications: Nucleic Acid Labeling & Oligo Synthesis

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Description

The amine-modified deoxyuridine 5'-triphosphate, aminoallyl-dUTP, can be used to produce amine-modified DNA by conventional enzymatic incorporation methods such as reverse transcription, nick translation, random primed labeling, or PCR. The amine-modified DNA that results can then be labeled with any amine-reactive dye or hapten. This two-step technique consistently results in a high degree of uniform DNA labeling that is difficult to obtain by other methods. Molecular Probes also provides a wide variety of amine-reactive reagents for labeling amine-modified DNA, including succinimidyl esters of our Alexa Fluor® dyes, conventional fluorescent dyes, biotin, and dinitrophenyl (DNP). Aminoallyl dUTP is also available in a 50 mM concentration (A32764), and aminoallyl dUTP, along with appropriate buffers, solvents and pre-measured amine-reactive dyes, is available in our convenient ARES™ DNA Labeling Kits.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Modification: Aminoallyl
Product Size: 500 µL
Concentration: 2 mM
Labeling Method: Indirect Labeling
Shipping Condition: Wet Ice

Contents & storage

Store in freezer (-5 to -30°C).

Documents

Manuals & protocols