Detect and measure cellular metabolites with Amplex Red assay kits »

Citations & References (12)

Invitrogen™

Amplex™ Red Catalase Assay Kit

For use with absorbance and fluorescence intensity techniques

Catalog Number	Quantity
A22180	400 Assays

Catalog number A22180

Price (USD)

526.00

Quantity:

400 Assays

Price (USD)

526.00

The Amplex Red Catalase Assay Kit provides a sensitive and simple fluorometric method for detecting as little as 50 mU/mL of catalase activity in a purified system in a 100μL assay volume.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Detection MethodAbsorbance

Dye TypeAmplex Red

Emission Wavelength585 nm

Excitation Wavelength571 nm

Quantity400 Assays

Shipping ConditionRoom Temperature

Substrate PropertiesChemical Substrate

Substrate TypeHRP (Horseradish Peroxidase) Substrate, Catalase Substrate

Target EnzymeCatalase

For Use With (Application)Enzyme and Metabolic Activity

Product LineAmplex

Product TypeCatalase Assay

Unit SizeEach

Contents & Storage

Store in freezer -5°C to -30°C and protect from light.

Frequently asked questions (FAQs)

I'm using an Amplex Red kit, the reagent changes color to pink almost immediately in my own Krebs-Ringer buffer but not in HBSS. Why is this?

The components of Krebs-Ringer buffer (salts) should not cause oxidation of the Amplex reagent (which, in the presence of peroxidase and H₂O₂ oxidizes to resorufin, which is pink in color and fluorescent). Try water alone (the water used to make the Krebs-Ringer buffer). Since Hank's Buffered Saline Solution is typically purchased rather than made in the lab, it likely would not have the same contaminant. Another option is to degas the buffer prior to use to removed dissolved oxygen radicals.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Can Amplex Red Assays be performed using cell lysates?

This is not recommended. The presence of endogenous proteases can complicate the assay by degrading the horseradish peroxidase (HRP). Endogenous peroxidases and antioxidants can modify the H₂O₂ required for the reaction, competing with HRP (and catalase) for the substrate.

The Amplex Red Assays are best performed with either purified enzymes or extracted H₂O₂ in a defined buffer system, extracellular solutions or body fluids (media, serum, etc.) that do not exhibit high levels of endogenous protease or oxidase activity and do not contain antioxidants.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Citations & References (12)

Citations & References

Abstract

RIP1 potentiates BPDE-induced transformation in human bronchial epithelial cells through catalase-mediated suppression of excessive reactive oxygen species.

Authors:Wang Q, Chen W, Xu X, Li B, He W, Padilla MT, Jang JH, Nyunoya T, Amin S, Wang X, Lin Y,

Journal:Carcinogenesis

PubMed ID:23633517

'Cell survival signaling is important for the malignant phenotypes of cancer cells. Although the role of receptor-interacting protein 1 (RIP1) in cell survival signaling is well documented, whether RIP1 is directly involved in cancer development has never been studied. In this report, we found that RIP1 expression is substantially increased ... More

Physiological characterization of Haemophilus influenzae Rd deficient in its glutathione-dependent peroxidase PGdx.

Authors:Pauwels F, Vergauwen B, Van Beeumen JJ

Journal:J Biol Chem

PubMed ID:14701867

'The chimeric peroxidase PGdx of Haemophilus influenzae Rd belongs to a recently identified family of thiol peroxidases capable of reducing hydrogen peroxide as well as alkylhydroperoxides by means of glutathione redox cycling. In the present study, we constructed a H. influenzae Rd strain, deficient in its PGdx encoding gene (open ... More

Receptor-interacting Protein 1 Increases Chemoresistance by Maintaining Inhibitor of Apoptosis Protein Levels and Reducing Reactive Oxygen Species through a microRNA-146a-mediated Catalase Pathway.

Authors:Wang Q, Chen W, Bai L, Chen W, Padilla MT, Lin AS, Shi S, Wang X, Lin Y,

Journal:

PubMed ID:24425875

'Although receptor-interacting protein 1 (RIP1) is well known as a key mediator in cell survival and death signaling, whether RIP1 directly contributes to chemotherapy response in cancer has not been determined. In this report, we found that, in human lung cancer cells, knockdown of RIP1 substantially increased cytotoxicity induced by ... More

Roles of catalase and hydrogen peroxide in green tea polyphenol-induced chemopreventive effects.

Authors:Yamamoto T, Lewis J, Wataha J, Dickinson D, Singh B, Bollag WB, Ueta E, Osaki T, Athar M, Schuster G, Hsu S

Journal:J Pharmacol Exp Ther

PubMed ID:14569057

'The green tea polyphenol (-)-epigallocatechin-3-gallate (EGCG) possesses promising anticancer potential. Although in vivo studies unveiled the metabolic routes and pharmacokinetics of EGCG and showed no adverse effects, in vitro studies at high concentrations demonstrated oxidative stress. EGCG causes differential oxidative environments in tumor versus normal epithelial cells, but the roles ... More

N-acetylcysteine reverses cardiac myocyte dysfunction in a rodent model of behavioral stress.

Authors:Chen F, Hadfield JM, Berzingi C, Hollander JM, Miller DB, Nichols CE, Finkel MS,

Journal:

PubMed ID:23722706

Compelling clinical reports reveal that behavioral stress alone is sufficient to cause reversible myocardial dysfunction in selected individuals. We developed a rodent stress cardiomyopathy model by a combination of prenatal and postnatal behavioral stresses (Stress). We previously reported a decrease in percent fractional shortening by echo, both systolic and diastolic ... More

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