Amplex™ Red Glucose/Glucose Oxidase Assay Kit

인용 및 참조 문헌 (21)

Invitrogen™

Amplex™ Red Glucose/Glucose Oxidase Assay Kit

Amplex Red Glucose/Glucose Oxidase Assay Kit는 100 μL 당 d-glucose 3 μM 또는 glucose oxidase 0.05 mU/mL까지 검출하는 민감도 높은자세히 알아보기

카탈로그 번호	수량
A22189	500 Assays

카탈로그 번호 A22189

제품 가격(KRW)

603,000

온라인 행사

Ends: 31-Dec-2025

669,000

할인액 66,000 (10%)

카트에 추가하기

500 Assays

제품 가격(KRW)

603,000

온라인 행사

Ends: 31-Dec-2025

669,000

할인액 66,000 (10%)

카트에 추가하기

Amplex Red Glucose/Glucose Oxidase Assay Kit는 100 μL 당 d-glucose 3 μM 또는 glucose oxidase 0.05 mU/mL까지 검출하는 민감도 높은 원스텝 형광측정법을 제공합니다.

For Research Use Only. Not for use in diagnostic procedures.

검출 방법Fluorescence

염료 유형Other Label(s) or Dye(s)

형식Tube(s), 96-well plate

수량500 Assays

배송 조건Room Temperature

용도(애플리케이션)Glucose/Glucose Oxidase Assay

용도 (장비)Microplate Reader, Spectrophotometer, Fluorometer

제품라인Amplex

제품 유형Amplex Red Assay Kit

Unit SizeEach

구성 및 보관

Store in freezer -5°C to -30°C and protect from light.

자주 묻는 질문(FAQ)

I'm using an Amplex Red kit, the reagent changes color to pink almost immediately in my own Krebs-Ringer buffer but not in HBSS. Why is this?

The components of Krebs-Ringer buffer (salts) should not cause oxidation of the Amplex reagent (which, in the presence of peroxidase and H₂O₂ oxidizes to resorufin, which is pink in color and fluorescent). Try water alone (the water used to make the Krebs-Ringer buffer). Since Hank's Buffered Saline Solution is typically purchased rather than made in the lab, it likely would not have the same contaminant. Another option is to degas the buffer prior to use to removed dissolved oxygen radicals.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Can Amplex Red Assays be performed using cell lysates?

This is not recommended. The presence of endogenous proteases can complicate the assay by degrading the horseradish peroxidase (HRP). Endogenous peroxidases and antioxidants can modify the H₂O₂ required for the reaction, competing with HRP (and catalase) for the substrate.

The Amplex Red Assays are best performed with either purified enzymes or extracted H₂O₂ in a defined buffer system, extracellular solutions or body fluids (media, serum, etc.) that do not exhibit high levels of endogenous protease or oxidase activity and do not contain antioxidants.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

인용 및 참조 문헌 (21)

인용 및 참조 문헌

Abstract

Development of a long-acting insulin analog using albumin fusion technology.

Authors:Duttaroy A, Kanakaraj P, Osborn BL, Schneider H, Pickeral OK, Chen C, Zhang G, Kaithamana S, Singh M, Schulingkamp R, Crossan D, Bock J, Kaufman TE, Reavey P, Carey-Barber M, Krishnan SR, Garcia A, Murphy K, Siskind JK, McLean MA, Cheng S, Ruben S, Birse CE, Blondel O

Journal:Diabetes

PubMed ID:15616036

The primary therapeutic goal for the treatment of diabetes is maintenance of a long-term, near-normoglycemic condition and prevention of the onset or progression of the complications associated with the disease. Although several analogs of human insulin have been developed, the currently prescribed long-acting insulin analogs do not provide a stable ... More

Perturbational profiling of a cell-line model of tumorigenesis by using metabolic measurements.

Authors:Ramanathan A, Wang C, Schreiber SL

Journal:Proc Natl Acad Sci U S A

PubMed ID:15840712

'Weinberg and coworkers have used serial transduction of a human, primary fibroblast cell line with the catalytic domain of human telomerase, large T antigen, small T antigen, and an oncogenic allele of H-ras to study stages leading toward a fully transformed cancerous state. We performed a three-dimensional screening experiment using ... More

Irs1 and Irs2 signaling is essential for hepatic glucose homeostasis and systemic growth.

Authors:Dong X, Park S, Lin X, Copps K, Yi X, White MF

Journal:J Clin Invest

PubMed ID:16374520

'Insulin receptor substrates, including Irs1 and Irs2, integrate insulin and IGF receptor signals with heterologous pathways to coordinate growth and metabolism. Since Irs2 is thought to be especially important in hepatic nutrient homeostasis, we deleted Irs2 [corrected] from hepatocytes of WT mice (called LKO) or genetically insulin-resistant Irs1-/- mice (called ... More

Stabilization of enzymes in silk films.

Authors:Lu S, Wang X, Lu Q, Hu X, Uppal N, Omenetto FG, Kaplan DL,

Journal:Biomacromolecules

PubMed ID:19323497

'Material systems are needed that promote stabilization of entrained molecules, such as enzymes or therapeutic proteins, without destroying their activity. We demonstrate that the unique structure of silk fibroin protein, when assembled into the solid state, establishes an environment that is conducive to the stabilization of entrained proteins. Enzymes (glucose ... More

Inhibition of mitochondrial pyruvate transport by zaprinast causes massive accumulation of aspartate at the expense of glutamate in the retina.

Authors:Du J, Cleghorn WM, Contreras L, Lindsay K, Rountree AM, Chertov AO, Turner SJ, Sahaboglu A, Linton J, Sadilek M, Satrústegui J, Sweet IR, Paquet-Durand F, Hurley JB,

Journal:

PubMed ID:24187136

'Transport of pyruvate into mitochondria by the mitochondrial pyruvate carrier is crucial for complete oxidation of glucose and for biosynthesis of amino acids and lipids. Zaprinast is a well known phosphodiesterase inhibitor and lead compound for sildenafil. We found Zaprinast alters the metabolomic profile of mitochondrial intermediates and amino acids ... More

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