LMP4 regulates Tbx5 protein subcellular localization and activity.
AuthorsCamarata T, Bimber B, Kulisz A, Chew TL, Yeung J, Simon HG
JournalJ Cell Biol
PubMed ID16880269
'The limb- and heart-specific Tbx5 transcription factor coexpresses with and directly binds to the novel PDZ-LIM domain protein, LMP4. LMP4 is distributed in the cytoplasm associated with the actin cytoskeleton. In the presence of LMP4, Tbx5 shuttles dynamically between the nucleus and cytoplasm and, in a complex with LMP4, localizes ... More
Multiphoton excitation spectra in biological samples.
AuthorsDickinson ME, Simbuerger E, Zimmermann B, Waters CW, Fraser SE
JournalJ Biomed Opt
PubMed ID12880336
'Multiphoton microscopy is becoming a popular mode of live and fixed cell imaging. This mode of imaging offers several advantages due to the fact that fluorochrome excitation is a nonlinear event resulting in excitation only at the plane of focus. Multiphoton excitation is enhanced by the use of ultrafast lasers ... More
Paper-supported 3D cell culture for tissue-based bioassays.
AuthorsDerda R, Laromaine A, Mammoto A, Tang SK, Mammoto T, Ingber DE, Whitesides GM,
JournalProc Natl Acad Sci U S A
PubMed ID19846768
Fundamental investigations of human biology, and the development of therapeutics, commonly rely on 2D cell-culture systems that do not accurately recapitulate the structure, function, or physiology of living tissues. Systems for 3D cultures exist but do not replicate the spatial distributions of oxygen, metabolites, and signaling molecules found in tissues. ... More
MAP2 is required for dendrite elongation, PKA anchoring in dendrites, and proper PKA signal transduction.
AuthorsHarada A, Teng J, Takei Y, Oguchi K, Hirokawa N
JournalJ Cell Biol
PubMed ID12163474
Microtubule-associated protein 2 (MAP2) is a major component of cross-bridges between microtubules in dendrites, and is known to stabilize microtubules. MAP2 also has a binding domain for the regulatory subunit II of cAMP-dependent protein kinase (PKA). We found that there is reduction in microtubule density in dendrites and a reduction ... More
Targeting of calcium:calmodulin signals to the cytoskeleton by IQGAP1.
AuthorsPsatha MI, Razi M, Koffer A, Moss SE, Sacks DB, Bolsover SR
JournalCell Calcium
PubMed ID17137623
Mast cells reorganize their actin cytoskeleton in response to cytosolic calcium signals while in parallel secreting histamine and other inflammatory mediators. The effect of calcium on actin is mediated in large part through calmodulin. EGFP-tagged calmodulin is concentrated in the actin-rich cortex of RBL-2H3 mast cells. Transfection with small interfering ... More
Critical roles for multiple formins during cardiac myofibril development and repair.
Cardiac and skeletal muscle function depends on the proper formation of myofibrils, which are tandem arrays of highly organized actomyosin contractile units called sarcomeres. How the architecture of these colossal molecular assemblages is established during development and maintained over the lifetime of an animal is poorly understood. We investigate the ... More
Rac-mediated macropinocytosis is a critical route for naked plasmid DNA transfer in mice.
AuthorsFumoto S, Nishi J, Ishii H, Wang X, Miyamoto H, Yoshikawa N, Nakashima M, Nakamura J, Nishida K,
JournalMol Pharm
PubMed ID19492848
We have recently discovered the potential for in vivo naked plasmid DNA (pDNA) transfer into gastric serosal surface cells in mice. As pDNA are huge molecules, the mechanism of gene transfer without carriers and physical forces is of great biological interest. The endocytic route for naked pDNA transfer into gastric ... More
In vivo imaging reveals dendritic targeting of laminated afferents by zebrafish retinal ganglion cells.
AuthorsMumm JS, Williams PR, Godinho L, Koerber A, Pittman AJ, Roeser T, Chien CB, Baier H, Wong RO
JournalNeuron
PubMed ID17114046
Targeting of axons and dendrites to particular synaptic laminae is an important mechanism by which precise patterns of neuronal connectivity are established. Although axons target specific laminae during development, dendritic lamination has been thought to occur largely by pruning of inappropriately placed arbors. We discovered by in vivo time-lapse imaging ... More
Posttranslational myristoylation of caspase-activated p21-activated protein kinase 2 (PAK2) potentiates late apoptotic events.
AuthorsVilas GL, Corvi MM, Plummer GJ, Seime AM, Lambkin GR, Berthiaume LG
JournalProc Natl Acad Sci U S A
PubMed ID16617111
p21-activated protein kinase (PAK) 2 is a small GTPase-activated serine/threonine kinase regulating various cytoskeletal functions and is cleaved by caspase-3 during apoptosis. We demonstrate that the caspase-cleaved PAK2 C-terminal kinase fragment (C-t-PAK2) is posttranslationally myristoylated, although myristoylation is typically a cotranslational process. Myristoylation and an adjacent polybasic domain of C-t-PAK2 ... More
Control of axon elongation via an SDF-1alpha/Rho/mDia pathway in cultured cerebellar granule neurons.
AuthorsArakawa Y, Bito H, Furuyashiki T, Tsuji T, Takemoto-Kimura S, Kimura K, Nozaki K, Hashimoto N, Narumiya S
JournalJ Cell Biol
PubMed ID12707308
Rho-GTPase has been implicated in axon outgrowth. However, not all of the critical steps controlled by Rho have been well characterized. Using cultured cerebellar granule neurons, we show here that stromal cell-derived factor (SDF)-1alpha, a neural chemokine, is a physiological ligand that can turn on two distinct Rho-dependent pathways with ... More
Phosphatidylinositol-3,4,5-trisphosphate regulates the formation of the basolateral plasma membrane in epithelial cells.
AuthorsGassama-Diagne A, Yu W, ter Beest M, Martin-Belmonte F, Kierbel A, Engel J, Mostov K
JournalNat Cell Biol
PubMed ID16921364
Polarity is a central feature of eukaryotic cells and phosphatidylinositol 3,4,5-trisphosphate (PtdIns(3,4,5)P3) has a central role in the polarization of neurons and chemotaxing cells. In polarized epithelial cells, PtdIns(3,4,5)P3 is stably localized at the basolateral plasma membrane, but excluded from the apical plasma membrane, as shown by localization of GFP ... More
MHC class II-peptide complexes and APC lipid rafts accumulate at the immunological synapse.
AuthorsHiltbold EM, Poloso NJ, Roche PA
JournalJ Immunol
PubMed ID12538693
Activation of CD4(+) Th cells requires their cognate interaction with APCs bearing specific relevant MHC class II-peptide complexes. This cognate interaction culminates in the formation of an immunological synapse that contains the various proteins and lipids required for efficient T cell activation. We now show that APC lipid raft membrane ... More
Two distinct distributions of F-actin are present in the hyphal apex of the oomycete Achlya bisexualis.
AuthorsYu YP, Jackson SL, Garrill A
JournalPlant Cell Physiol
PubMed ID15047875
We show that two distinct distributions of F-actin are present in the hyphal apex of the oomycete Achlya bisexualis, that have been chemically fixed with a combination of methylglyoxal and formaldehyde and stained with Alexa phalloidin. In approximately one half of the hyphae examined, an F-actin depleted zone within the ... More
Phosphatidylinositol 3-kinases in carcinoembryonic antigen-related cellular adhesion molecule-mediated internalization of Neisseria gonorrhoeae.
Neisseria gonorrhoeae can be internalized by mammalian cells through interactions between bacterial opacity-associated (Opa) adhesins and members of the human carcinoembryonic antigen-related cellular adhesion molecule (CEACAM) family. We examined the role of phosphatidylinositol 3-kinases (PI3Ks) in gonococcal invasion of epithelial cell lines expressing either CEACAM1 or CEACAM3. CEACAM3-mediated internalization, but ... More
Targeting of amacrine cell neurites to appropriate synaptic laminae in the developing zebrafish retina.
AuthorsGodinho L, Mumm JS, Williams PR, Schroeter EH, Koerber A, Park SW, Leach SD, Wong RO
JournalDevelopment
PubMed ID16258076
Cellular mechanisms underlying the precision by which neurons target their synaptic partners have largely been determined based on the study of projection neurons. By contrast, little is known about how interneurons establish their local connections in vivo. Here, we investigated how developing amacrine interneurons selectively innervate the appropriate region of ... More
Role of Dicer and Drosha for endothelial microRNA expression and angiogenesis.
AuthorsKuehbacher A, Urbich C, Zeiher AM, Dimmeler S
JournalCirc Res
PubMed ID17540974
MicroRNAs (miRNAs) are small noncoding RNAs that regulate gene expression by binding to the cellular transcript leading to translational repression or degradation of the target mRNA. Dicer and Drosha are the miRNA processing enzymes that are required for the maturation of miRNAs. Here, we investigated the role of Dicer and ... More
Micropatterning Alginate Substrates for in vitro Cardiovascular Muscle on a Chip.
Authors
JournalAdv Funct Mater
PubMed ID26213529
RUFY3 and RUFY4 are ARL8 effectors that promote coupling of endolysosomes to dynein-dynactin.
Authors
JournalNat Commun
PubMed ID35314674
RIPK1 and Caspase-8 Ensure Chromosome Stability Independently of Their Role in Cell Death and Inflammation.
Authors
JournalMol Cell
PubMed ID30598363
Sequential Ras/MAPK and PI3K/AKT/mTOR pathways recruitment drives basal extrusion in the prostate-like gland of Drosophila.
Authors
JournalNat Commun
PubMed ID32385236
Proteasome stress in skeletal muscle mounts a long-range protective response that delays retinal and brain aging.
Authors
JournalCell Metab
PubMed ID33773104
Independent Control of Topography for 3D Patterning of the ECM Microenvironment.
Authors
JournalAdv Mater
PubMed ID26551393
Cancer Cells Employ Nuclear Caspase-8 to Overcome the p53-Dependent G2/M Checkpoint through Cleavage of USP28.
Authors
JournalMol Cell
PubMed ID31982308
Asymmetric division of contractile domains couples cell positioning and fate specification.
Authors
JournalNature
PubMed ID27487217
Histone demethylase Lsd1 is required for the differentiation of neural cells in Nematostella vectensis.
Authors
JournalNat Commun
PubMed ID35075108
Cell-to-cell expression variability followed by signal reinforcement progressively segregates early mouse lineages.