Internalization and degradation of macrophage Fc receptors bound to polyvalent immune complexes.
AuthorsMellman I, Plutner H
JournalJ Cell Biol
PubMed ID6715404
'We have studied the Fc receptor-mediated pinocytosis of immunoglobulin G (IgG)-containing immune complexes by mouse macrophages. IgG complexes were formed from affinity-purified rabbit dinitrophenyl IgG and dinitrophenyl modified BSA at molar ratios of 2.5-10:1. Both the specificity of binding and the fate of internalized receptors were analyzed using monoclonal and ... More
More sensitive immune complex transfer enzyme immunoassay for antibody IgG to p17 of HIV-1 with shorter incubation time for immunoreactions and larger volumes of serum samples.
AuthorsIshikawa S, Hashida S, Hashinaka K, Kojima M, Saito A, Takamizawa A, Shinagawa H, Oka S, Shimada K, Ishikawa E
JournalJ Clin Lab Anal
PubMed ID9292391
'In the previous immune complex transfer enzyme immunoassay for antibody IgG to p17 of HIV-1, the immune complex comprising 2,4-dinitrophenyl-bovine serum albumin-recombinant p17 conjugate, anti-p17 IgG, and recombinant p17-beta-D-galactosidase conjugate was trapped onto polystyrene beads coated with (anti-2,4-dinitrophenyl group) IgG by overnight incubation and was transferred to polystyrene beads coated ... More
Behavior of the idiotypic network in conventional immune responses. III. Detection and enumeration of cells producing idiotypic and anti-idiotypic antibodies by a spot ELISA technique.
AuthorsSegre M, Segre D
JournalCell Immunol
PubMed ID7954841
'The idiotypic (Ab1) and anti-idiotypic (Ab2) responses of spleen cells of mice immunized with DNP-Ficoll were assayed with the spot ELISA technique in wells coated with DNP-BSA or with affinity-purified rabbit anti-DNP antibodies, respectively. In agreement with results previously obtained with the hemolytic plaque technique, large numbers of Ab1 and ... More
Detection of one attomole of [Arg8]-vasopressin by novel noncompetitive enzyme immunoassay (hetero-two-site complex transfer enzyme immunoassay).
AuthorsHashida S, Tanaka K, Yamamoto N, Uno T, Yamaguchi K, Ishikawa E
JournalJ Biochem (Tokyo)
PubMed ID1778973
'One attomole of [Arg8]-vasopressin (AVP) was detected by a novel noncompetitive enzyme immunoassay (hetero-two-site complex transfer enzyme immunoassay). AVP was indirectly biotinylated using N-hydroxysuccinimidobiotin and trapped onto an anti-AVP IgG-coated polystyrene ball. After washing, biotinylated AVP was eluted from the polystyrene ball with HCl and was reacted with 2,4-dinitrophenyl-fluorescein disulfide-bovine ... More
Quantitative measurement of mast cell degranulation using a novel flow cytometric annexin-V binding assay.
AuthorsDemo SD, Masuda E, Rossi AB, Throndset BT, Gerard AL, Chan EH, Armstrong RJ, Fox BP, Lorens JB, Payan DG, Scheller RH, Fisher JM
JournalCytometry
PubMed ID10404150
'BACKGROUND: Mast cells are primary mediators of allergic inflammation. Antigen-mediated crosslinking of their cell surface immunoglobulin E (IgE) receptors results in degranulation and the release of proinflammatory mediators including histamine, tumor necrosis factor-alpha, and leukotrienes. METHODS: Mast cells were stimulated to degranulate by using either IgE crosslinking or ionophore treatment. ... More
Compartmentalized IgE receptor-mediated signal transduction in living cells.
AuthorsStauffer TP, Meyer T
JournalJ Cell Biol
PubMed ID9396750
'Several receptor-mediated signal transduction pathways, including EGF and IgE receptor pathways, have been proposed to be spatially restricted to plasma membrane microdomains. However, the experimental evidence for signaling events in these microdomains is largely based on biochemical fractionation and immunocytochemical studies and only little is known about their spatial dynamics ... More
Activation of protein kinase C in rat basophilic leukemia cells stimulates increased production of phosphatidylinositol 4-phosphate and phosphatidylinositol 4,5-bisphosphate: correlation with actin polymerization.
AuthorsApgar JR
JournalMol Biol Cell
PubMed ID7749199
'Cross-linking of the immunoglobulin E receptor on rat basophilic leukemia (RBL)1 cells by multivalent antigen activates phosphatidylinositol (PI) kinase and phosphatidylinositol 4-phosphate (PIP) kinase leading to the increased production of PIP and phosphatidylinositol 4,5-bisphosphate (PIP2). Activators of protein kinase C (PKC), such as phorbol myristate acetate (PMA) and the synthetic ... More
Suppression of TNF-alpha secretion by azelastine in a rat mast (RBL-2H3) cell line: evidence for differential regulation of TNF-alpha release, transcription, and degranulation.
AuthorsHide I, Toriu N, Nuibe T, Inoue A, Hide M, Yamamoto S, Nakata Y
JournalJ Immunol
PubMed ID9300717
'The mast cell plays a pivotal role in initiating allergic inflammation by secreting several cytokines including TNF-alpha, in addition to granule mediators such as histamine. Anti-allergic drugs including azelastine prevent immediate-type hypersensitivity by inhibiting mast cell degranulation, as well as blocking histamine H1 receptors. However, their effects on cytokine release ... More
Fc epsilon receptor mediated Ca2+ influx into mast cells is modulated by the concentration of cytosolic free Ca2+ ions.
AuthorsDar O, Pecht I
JournalFEBS Lett
PubMed ID1397260
'The relationship between the Fc epsilon receptor mediated stimulation of mast cells and the Ca2+ signal it induces were studied using thapsigargin (TG), a blocker of the endoplasmic reticulum Ca2+ pump. TG induced, in mucosal mast cells (RBL-2H3 line), a dose-dependent and an InsP3-independent increase in [Ca2+]i (from resting levels ... More
Leucine zipper dimerized bivalent and bispecific scFv antibodies from a semi-synthetic antibody phage display library.
Authorsde Kruif J, Logtenberg T
JournalJ Biol Chem
PubMed ID8631798
'This report describes the construction of leucine zipper-based dimerization cassettes for the conversion of recombinant monomeric scFv antibody fragments to bivalent and bispecific dimers. A truncated murine IgG3 hinge region and a Fos or Jun leucine zipper were cloned into four scFv fragments previously isolated from a synthetic antibody phage ... More
Clustering, mobility, and triggering activity of small oligomers of immunoglobulin E on rat basophilic leukemia cells.
AuthorsMenon AK, Holowka D, Webb WW, Baird B
JournalJ Cell Biol
PubMed ID2418032
'We have recently shown that small oligomers of IgE bound to univalent receptors for IgE on the surface of rat basophilic leukemia cells induce extensive aggregation of the receptors at 4 degrees C into patches resolvable by fluorescence microscopy and that this does not occur with monomeric IgE (Menon, A. ... More
Redistribution of plasma membrane proteins by electroosmosis elicits cytosolic calcium response in tumor mast cells.
AuthorsFeder TJ, Webb WW
JournalJ Cell Physiol
PubMed ID7962106
'Activation of mast cells and basophils by binding of ligands that crosslink and micro-aggregate cell surface receptors leads to a series of responses including a phosphoinositide cascade, elevation of intracellular free calcium ([Ca2+]i), morphological changes in the cell plasma membrane, and ultimately, exocytosis of granules containing histamine and other mediators ... More
Kinetics of multivalent antigen DNP-BSA binding to IgE-Fc epsilon RI in relationship to the stimulated tyrosine phosphorylation of Fc epsilon RI.
AuthorsXu K, Goldstein B, Holowka D, Baird B
JournalJ Immunol
PubMed ID9531278
'Multivalent DNP-BSA is commonly used to cross-link anti-DNP IgE bound to Fc epsilon RI to stimulate cellular responses, although key features of the binding process are unknown. Fluorescence quenching can be used to study the kinetics of DNP-BSA binding to FITC-IgE. We observe that DNP-BSA binds more slowly to IgE ... More
Characterization of protein-tyrosine phosphatases that dephosphorylate the high affinity IgE receptor.
AuthorsMao SY, Metzger H
JournalJ Biol Chem
PubMed ID9162030
'An early event that follows aggregation of the high affinity receptor for IgE (FcepsilonRI) is the phosphorylation of protein tyrosines, especially those on the beta- and gamma-subunits of the receptor. Disaggregation of the receptors leads to their rapid dephosphorylation, but even stably aggregated receptors undergo continual rounds of phosphorylation and ... More
Cross-linking of receptor-bound IgE to aggregates larger than dimers leads to rapid immobilization.
AuthorsMenon AK, Holowka D, Webb WW, Baird B
JournalJ Cell Biol
PubMed ID2935543
'Controlled cross-linking of IgE-receptor complexes on the surface of rat basophilic leukemia cells and mast cells has allowed a comparison of the lateral mobility and cell triggering activity of monomers, dimers, and higher oligomers of receptors. Addition of a monoclonal anti-IgE(Fc) antibody to IgE-sensitized cells in stoichiometric amounts relative to ... More
Surface functions during mitosis in rat basophilic leukemia cells.
'At the entry into mitosis, cells abruptly lose membrane activities such as phagocytosis, pinocytosis, and capping. The present studies test if mitotic cells also resist functional responses to cell surface ligand-receptor interactions. The IgE receptors of RBL-2H3 rat basophilic leukemia cells were labeled with anti-dinitrophenol IgE (anti-DNP-IgE) and then cross-linked ... More
Assessment of IgE-receptor function through measurement of hydrolysis of membrane inositol phospholipids. New insights on the phenomena of biphasic antigen concentration-response curves and desensitization.
AuthorsMaeyama K, Hohman RJ, Ali H, Cunha-Melo JR, Beaven MA
JournalJ Immunol
PubMed ID2836506
'Previous studies have shown that hydrolysis of membrane inositol phospholipids in rat basophilic leukemia (RBL-2H3) cells depended on the rate and extent of the aggregation of receptors of IgE. This response was used as an experimental probe to study the role of IgE receptors in initiating stimulatory and inhibitory processes ... More
Wortmannin-sensitive phosphorylation, translocation, and activation of PLCgamma1, but not PLCgamma2, in antigen-stimulated RBL-2H3 mast cells.
AuthorsBarker SA, Caldwell KK, Pfeiffer JR, Wilson BS
JournalMol Biol Cell
PubMed ID9450969
'In RBL-2H3 tumor mast cells, cross-linking the high affinity IgE receptor (FcepsilonRI) with antigen activates cytosolic tyrosine kinases and stimulates Ins(1,4,5)P3 production. Using immune complex phospholipase assays, we show that FcepsilonRI cross-linking activates both PLCgamma1 and PLCgamma2. Activation is accompanied by the increased phosphorylation of both PLCgamma isoforms on serine ... More
In vitro inhibition, by loratadine and descarboxyethoxyloratadine, of histamine release from human basophils, and of histamine release and intracellular calcium fluxes in rat basophilic leukemia cells (RBL-2H3).
AuthorsBerthon B, Taudou G, Combettes L, Czarléwski W, Carmi-Leroy A, Marchand F, Weyer A
JournalBiochem Pharmacol
PubMed ID7510965
'The effect of the H1-antihistamine drug loratadine and its active metabolite descarboxyethoxyloratadine upon histamine release was examined on anti-immunoglobulin E (IgE) triggered human basophils and 2,4-dinitrophenyl (DNP) triggered rat basophilic leukemia (RBL-2H3) cells. In both experimental systems, dose-dependent inhibition of histamine release was observed at descarboxyethoxyloratadine and loratadine doses above ... More
The control of mediator release from RBL-2H3 cells: roles for Ca2+, Na+, and protein kinase C1.
AuthorsStump RF, Oliver JM, Cragoe EJ, Deanin GG
JournalJ Immunol
PubMed ID3598191
'Antigen-stimulated rat basophilic leukemia (RBL-2H3) cells release serotonin and other inflammatory mediators by a process that requires Ca2+ influx and increased cytoplasmic Ca2+ levels, and is mimicked by Ca2+ ionophores. We report here that the Ca2+ response to antigen and to ionomycin has two components, a Ca2+ spike and a ... More
Targeting Janus kinase 3 in mast cells prevents immediate hypersensitivity reactions and anaphylaxis.
AuthorsMalaviya R, Zhu D, Dibirdik I, Uckun FM
JournalJ Biol Chem
PubMed ID10480916
'Janus kinase 3 (JAK3), a member of the Janus family protein-tyrosine kinases, is expressed in mast cells, and its enzymatic activity is enhanced by IgE receptor/FcepsilonRI cross-linking. Selective inhibition of JAK3 in mast cells with 4-(4''-hydroxylphenyl)-amino-6, 7-dimethoxyquinazoline) (WHI-P131) blocked the phospholipase C activation, calcium mobilization, and activation of microtubule-associated protein ... More
Association of the crosslinked IgE receptor with the membrane skeleton is independent of the known signaling mechanisms in rat basophilic leukemia cells.
AuthorsApgar JR
JournalCell Regul
PubMed ID1830493
'Crosslinking of the IgE receptor on the surface of rat basophilic leukemia (RBL) cells by multivalent antigen induces an association of these receptors with the detergent-insoluble membrane skeleton. Detergent insolubility of the receptor can also be induced on purified plasma membranes isolated from RBL cells by the use of either ... More
Measurement of cytosolic free Ca2+ in individual small cells using fluorescence microscopy with dual excitation wavelengths.
AuthorsTsien RY, Rink TJ, Poenie M
JournalCell Calcium
PubMed ID3874696
'Free Ca2+ concentrations in the cytosol of individual small cells can be recorded with a new fluorescent Ca2+ indicator, "fura-2", and a fluorescence microscope modified to chop rapidly between two wavelengths of excitation. Both fura-2 and its Ca2+ complex fluoresce strongly, but their excitation peaks differ in wavelength. Alternation between ... More
Lung injury after deposition of IgA immune complexes. Requirements for CD18 and L-arginine.
AuthorsMulligan MS, Warren JS, Smith CW, Anderson DC, Yeh CG, Rudolph AR, Ward PA
JournalJ Immunol
PubMed ID1374449
'Acute lung injury produced by deposition of IgA immune complexes is complement-dependent, neutrophil-independent, oxygen radical-mediated, and may be a result of the formation of the hydroxyl radical (HO) generated directly or indirectly from activated lung macrophages. The current studies were designed to evaluate further the pathophysiologic events that occur after ... More
Translocation of protein kinase C is not required to inhibit the antigen-induced increase of cytosolic calcium in a mast cell line.
AuthorsErne P, Mazurek N, Borner C, Conscience JF, Eppenberger U, Fabbro D
JournalBiochem Biophys Res Commun
PubMed ID3103618
'Cross-linking of receptor bound IgE antibodies by multivalent antigen (DNP8-BSA) on PB-3c cells leads to an increase of cytosolic calcium ((Ca2+)i). Active tumor promoting phorbol esters and teleocidin which specifically activate the phospholipid Ca2+-sensitive protein kinase (PKC), inhibited the antigen-mediated rise in (Ca2+)i and induced a time and dose-dependent translocation ... More
Binding of IgG to MoFc gamma RII purified and reconstituted into supported planar membranes as measured by total internal reflection fluorescence microscopy.
AuthorsPoglitsch CL, Sumner MT, Thompson NL
JournalBiochemistry
PubMed ID1829634
'Total internal reflection fluorescence microscopy (TIRFM) has been combined with functional reconstitution of the mouse IgG receptor moFc gamma RII in substrate-supported planar membranes to quantitatively probe IgG-moFc gamma RII interactions. MoFc gamma RII was purified from the macrophage-related cell line J774A.1 using affinity chromatography with Fab fragments of the ... More
Functional effects of overexpression of protein kinase C-alpha, -beta, -delta, -epsilon, and -eta in the mast cell line RBL-2H3.
'The rat basophilic leukemic (RBL-2H3) cell line was stably transfected with the endogenously expressed Ca2+-dependent protein kinase C-alpha (PKC-alpha) and -betaI and the Ca2+-independent delta and epsilon isoforms to study their functional roles. In addition, the Ca2+-independent PKC-eta was expressed. All transfected PKC isoforms translocated to the membrane-containing fraction in ... More
In vitro fusion of endosomes following receptor-mediated endocytosis.
AuthorsDiaz R, Mayorga L, Stahl P
JournalJ Biol Chem
PubMed ID3360775
'Receptor-mediated endocytosis and receptor recycling involve a series of intracellular membrane fusion events that appear to play an important role in the regulation of the overall rate and efficiency of the process. An endosome-endosome fusion assay is described using two ligands that (i) rapidly and efficiently enter the endosomal compartment ... More
Study of the transit of an integral membrane protein from secretory granules through the plasma membrane of secreting rat basophilic leukemia cells using a specific monoclonal antibody.
AuthorsBonifacino JS, Perez P, Klausner RD, Sandoval IV
JournalJ Cell Biol
PubMed ID3511074
'The monoclonal antibody 5G10 reacted specifically with an 80-kD integral membrane protein in rat basophilic leukemia (RBL) cells. Immunofluorescence microscopy studies of RBL cells, fixed and permeabilized, revealed that the 80-kD protein was located in the membrane of cytoplasmic vesicles. The vesicles were identified as secretory granules by their content ... More
A single cell observation of staurosporine effect on the Ca2+ signals in rat basophilic leukemia cells.
AuthorsTeshima R, Ikebuchi H, Terao T, Miyagawa T, Arata Y, Nakanishi M
JournalFEBS Lett
PubMed ID2226774
'A digital imaging fluorescence microscope was used to study the effect of a protein kinase inhibitor staurosporine on the antigen-dependent calcium signals in an individual rat basophilic leukemia cell (RBL-2H3). Although dose dependency of staurosporine was different from one cell to another, staurosporine inhibited, at low concentration, the calcium influx ... More
The Ca signal from fura-2 loaded mast cells depends strongly on the method of dye-loading.
AuthorsAlmers W, Neher E
JournalFEBS Lett
PubMed ID3840439
'The Ca concentration ([Ca2+]i) in single rat peritoneal mast cells was measured by means of the new fluorescent Ca-indicator dye fura-2. Dye-loaded cells were made to degranulate with either antigen or compound 48/80. In cells loaded with extracellularly applied, membrane-permeant fura-2 ester, degranulation was accompanied by a permanent loss of ... More
Ca2+ fluxes and protein phosphorylation in stimulus-secretion coupling of basophils.
AuthorsSagi-Eisenberg R, Mazurek N, Pecht I
JournalMol Immunol
PubMed ID6084172
Transient accumulation of new class II MHC molecules in a novel endocytic compartment in B lymphocytes.
AuthorsAmigorena S, Drake JR, Webster P, Mellman I
JournalNature
PubMed ID8177316
Endocytosis of antigen by antigen-presenting cells results in the production of peptides that bind to newly synthesized class II molecules of the major histocompatibility complex. A new population of class II-enriched vesicles has been discovered in B lymphocytes that accumulate internalized antigen but are distinct from endosomes and lysosomes. These ... More
Calcium influx in a single rat basophilic leukemia cell as revealed with a digital imaging fluorescence microscope.
AuthorsKato K, Nakanishi M, Arata Y, Teshima R, Terao T, Miyamoto H
JournalJ Biochem (Tokyo)
PubMed ID3667562
Using a digital imaging fluorescence microscope, we have detected a rapid transient increase in the free cytosolic calcium concentration in a single rat basophilic leukemia cell (RBL-2H3) after antigen stimulation. Calcium ions were transported very rapidly (within 1 s) after a lag time (about 10 s at 37 degrees C) ... More
Fast calcium transients in rat peritoneal mast cells are not sufficient to trigger exocytosis.
AuthorsNeher E, Almers W
JournalEMBO J
PubMed ID3082623
The calcium concentration, [Ca]i, in single rat peritoneal mast cells was measured by means of the new Ca indicator dye fura-2. Upon stimulation with antigen or compound 48/80, [Ca]i rose for seconds to values greater than 5 microM. These Ca transients did not depend on the presence of extracellular Ca, ... More
Characterization of a new type of variant of rat basophilic leukemia 2H3 cells presenting a different pattern of calcium signal.
AuthorsKato K, Nakanishi M, Arata Y, Teshima R, Terao T, Miyamoto H, Hanaoka F, Ui M, Masuzawa Y, Hiraishi S
JournalExp Cell Res
PubMed ID2159404
We selected a new type of variant (designated 3C7) derived spontaneously from parental RBL-2H3 cells. 3C7 cells showed lower contact inhibition, anchorage dependency, and serotonin release activity than those of RBL-2H3 cells. We conclude that 3C7 cells are a transformant of RBL-2H3 cell with greater malignancy. The production of inositol ... More
Microfilaments regulate the rate of exocytosis in rat basophilic leukemia cells.
AuthorsNarasimhan V, Holowka D, Baird B
JournalBiochem Biophys Res Commun
PubMed ID2203345
Disruption of microfilaments in rat basophilic leukemia (RBL) cells by exposure to cytochalasin B is observed to potentiate the rate of antigen-stimulated secretion from these cells. Under these conditions, cytochalasin B is without effect on the antigen-stimulated production of inositol phosphates or 45Ca2(+)-influx. In streptolysin-O-permeabilized RBL cells, cytochalasin B is ... More
GTP gamma S stimulation of endosome fusion suggests a role for a GTP-binding protein in the priming of vesicles before fusion.
AuthorsMayorga LS, Diaz R, Colombo MI, Stahl PD
JournalCell Regul
PubMed ID2519610
Guanosine 5'-(3-O-thio)triphosphate (GTP gamma S), a non-hydrolyzable analogue of GTP, inhibits in vitro fusion among early endocytic vesicles in the presence of high concentrations of cytosol. In this report we show that fusion is remarkably stimulated by GTP gamma S under conditions where cytosolic components are the limiting factors for ... More
Simultaneous determination of intracellular calcium concentration and histamine secretion in rat basophilic leukemia cells (RBL-2H3).
AuthorsMaeyama K, Sasaki M, Watanabe T
JournalAnal Biochem
PubMed ID1713750
In rat basophilic leukemia cells (RBL-2H3), a tumor analogue of mast cells, the aggregation of IgE receptors initiates increase in the intracellular concentration of calcium ([Ca2+]i), monitored with the fluorescent Ca probe fura-2, and finally results in histamine secretion. In cell suspensions, however, the fluorescence gradually increases due to leakage ... More
Actin restricts FcepsilonRI diffusion and facilitates antigen-induced receptor immobilization.
AuthorsAndrews NL, Lidke KA, Pfeiffer JR, Burns AR, Wilson BS, Oliver JM, Lidke DS,
JournalNat Cell Biol
PubMed ID18641640
The actin cytoskeleton has been implicated in restricting diffusion of plasma membrane components. Here, simultaneous observations of quantum dot-labelled FcepsilonRI motion and GFP-tagged actin dynamics provide direct evidence that actin filament bundles define micron-sized domains that confine mobile receptors. Dynamic reorganization of actin structures occurs over seconds, making the location ... More
A sensitive competitive ELISA for 2,4-dinitrophenol using 3,6-fluorescein diphosphate as a fluorogenic substrate.
AuthorsHuang Z, Olson NA, You W, Haugland RP
JournalJ Immunol Methods
PubMed ID1301064
A competitive ELISA for sensitive detection of 2,4-dinitrophenol (DNP) was established. Certain amounts of bovine serum albumin conjugate of DNP were readily coated on a polystyrene microplate. Free DNP was then quantitated by its competition with the coated DNP for binding to anti-DNP (antibody)-alkaline phosphatase conjugate. The enzyme conjugate remaining ... More
Fc receptor isoforms exhibit distinct abilities for coated pit localization as a result of cytoplasmic domain heterogeneity.
AuthorsMiettinen HM, Rose JK, Mellman I
JournalCell
PubMed ID2568890
Mouse macrophages and lymphocytes express two distinct isoforms of a single class of Fc receptor for IgG. The macrophage isoform (FcRII-B2) is identical to the lymphocyte isoform (FcRII-B1) except for an inframe insertion in the cytoplasmic tail of FcRII-B1 that increases its length from 47 to 94 amino acids. To ... More
Receptor and protein kinase C-mediated regulation of ARF binding to the Golgi complex.
AuthorsDe Matteis MA, Santini G, Kahn RA, Di Tullio G, Luini A
JournalNature
PubMed ID7689177
The formation of constitutive transport vesicles involves the association of non-clathrin coat proteins to transport organelles. Here we report that IgE receptors and protein kinase C (PKC) regulate the GTP-dependent binding of the two coat proteins ADP-ribosylation factor (ARF) and beta-COP to Golgi membranes in rat basophilic leukaemia cells. Activation ... More
Raft-partitioning of the ubiquitin ligases Cbl and Nedd4 upon IgE-triggered cell signaling.
AuthorsLafont F, Simons K
JournalProc Natl Acad Sci U S A
PubMed ID11248052
The high affinity receptor for IgE, FcepsilonRI on mast cells and basophils plays an essential role in immunological defense. Upon multivalent antigen binding, FcepsilonRI becomes phoshorylated by the protein-tyrosine kinase Lyn, as a result of receptor clustering in lipid rafts. FcepsilonRI has been shown to be ubiquitinated. Ubiquitination can lead ... More
A flow microfluorimetric analysis of the binding of immunoglobulins to Fc gamma receptors on brush borders of the neonatal mouse jejunal epithelium.
AuthorsMacKenzie NM, Keeler KD
JournalImmunology
PubMed ID6698578
In order to determine whether the neonatal mouse small intestine has the ability to be selective in the binding of homologous immunoglobulin isotypes, mouse immunoglobulins were tested for their ability to bind to brush borders isolated from jejunal enterocytes of neonatal and adult mice. The binding of immunoglobulins to brush ... More
Fast receptor-induced formation of glycerophosphoinositol-4-phosphate, a putative novel intracellular messenger in the Ras pathway.
AuthorsFalasca M, Carvelli A, Iurisci C, Qiu RG, Symons MH, Corda D
JournalMol Biol Cell
PubMed ID9188097
Glycerophosphoinositols are phosphoinositide metabolites whose levels are constitutively elevated in Ras-transformed cells. Here, we show that one of these compounds, glycerophosphoinositol-4-phosphate (GroPIns-4-P) responds acutely to the stimulation of the epidermal growth factor receptor, with a fast, massive and transient increase. The mechanism leading to GroPIns-4-P formation involves the activation of ... More
Modulation of IgG subclass expression during antibody responses in sheep.
AuthorsKerlin RL, Watson DL
JournalRes Vet Sci
PubMed ID2463655
Experiments were undertaken to investigate IgG subclass expression during epitope-specific antibody responses in sheep. Animals were immunised with conjugates of bovine serum albumin-DNP (BSA-DNP), or killed Staphylococcus aureus-DNP (Sa-DNP) alone or with muramyl dipeptide (MDP), dextran sulphate (DXS), or staphylococcal exotoxins (toxin). Sheep received two injections of the same preparation ... More
Inhibition of Lyn function in mast cell activation by SH3 domain binding peptides.
AuthorsStauffer TP, Martenson CH, Rider JE, Kay BK, Meyer T
JournalBiochemistry
PubMed ID9235982
While Lyn tyrosine kinase has been shown to be necessary for IgE-receptor (FcepsilonRI)-mediated mast cell activation, the mechanism of Lyn activation is not yet understood. Using a micro-electroporation technique to quantitatively introduce peptides into the cytosol of tumor mast cells, we show that proline-rich peptides that preferentially bind Src family ... More
Spreading of human neutrophils is immediately preceded by a large increase in cytoplasmic free calcium.
AuthorsKruskal BA, Shak S, Maxfield FR
JournalProc Natl Acad Sci U S A
PubMed ID3458251
When human polymorphonuclear leukocytes (PMN) are placed on various surfaces, they attach and spread rapidly, increasing their diameter severalfold. The spreading is associated with extensive changes in the cytoskeleton. Since many cytoskeletal events are regulated by Ca2+, we measured the cytosolic free calcium concentration ([Ca2+]i) in individual human PMN as ... More
Biochemical analysis of glucocorticoid-induced inhibition of IgE-mediated histamine release from mouse mast cells.
AuthorsDaëron M, Sterk AR, Hirata F, Ishizaka T
JournalJ Immunol
PubMed ID6180000
Pretreatment of mouse mast cells with 10(-7) to 10(-6) M dexamethasone (DM) during overnight sensitization with mouse IgE antibody resulted in inhibition of antigen-induced histamine release and degranulation. The inhibition of both degranulation and histamine release increased linearly with the duration of the treatment; maximal inhibition was obtained after approximately ... More
Constitutive and inducible mechanisms for synthesis and release of cytokines in immune cell lines.
AuthorsBaumgartner RA, Deramo VA, Beaven MA
JournalJ Immunol
PubMed ID8892643
We found that production and release of two functionally antagonistic cytokines, TGF-beta and TNF-alpha, were regulated differently in the mast cell, T cell, and macrophage cell lines RBL-2H3, MLA-144, and U-937, respectively. TGF-beta was produced and released constitutively in all three cell lines. When, however, the cell lines were stimulated ... More
The presence of two Fc receptors on mouse macrophages: evidence from a variant cell line and differential trypsin sensitivity.
AuthorsUnkeless JC
JournalJ Exp Med
PubMed ID323399
A stable variant of a clone of the P388D1 macrophage line was isolated using four cycles of treatment with mouse IgG2a-rabbit anti-kappa complexes and rabbit complement. The variant had the same Ka and about the same number of sites per cell for IgG2a as the parent line. However, the variant ... More
Transport of phagosomal components to an endosomal compartment.
AuthorsPitt A, Mayorga LS, Schwartz AL, Stahl PD
JournalJ Biol Chem
PubMed ID1730578
The participation of phagosomes in interorganellar protein and membrane exchange is important to the maturation of phagosomes into phagolysosomes. To investigate this process, we have developed an assay to measure protein transport from phagosomes to other vesicle populations. J774-E clone macrophages phagocytosed 125I-anti-dinitrophenol IgG-coated Staphylococcus aureus for 3 min followed ... More
Direct demonstration of increased intracellular concentration of free calcium as measured by quin-2 in stimulated rat peritoneal mast cell.
AuthorsWhite JR, Ishizaka T, Ishizaka K, Sha'afi R
JournalProc Natl Acad Sci U S A
PubMed ID6204334
Rat mast cells, passively sensitized with monoclonal mouse IgE antibody, were stimulated with multi-valent antigen, and an increase in cytoplasmic Ca2+ was determined by using the fluorescent probe quin-2. The increase in quin-2 fluorescence reached maximum within 20 sec after the antigen challenge and then gradually declined. A substantial increase ... More
Endocytosis in skeletal muscle fibers.
AuthorsKaisto T, Rahkila P, Marjomäki V, Parton RG, Metsikkö K
JournalExp Cell Res
PubMed ID10585279
Defining the organization of endocytic pathway in multinucleated skeletal myofibers is crucial to understand the routing of membrane proteins, such as receptors and glucose transporters, through this system. Here we analyzed the organization of the endocytic trafficking pathways in isolated rat myofibers. We found that sarcolemmal-coated pits and transferrin receptors ... More
A novel action of glucocorticosteroid in inhibition of leukotriene C4 production by rat basophilic leukemia cells: suppression of the elevation of cytosolic free Ca2+ induced by antigen.
AuthorsHer E, Weissman BA, Zor U
JournalBiochim Biophys Acta
PubMed ID2310771
Rat basophilic leukemia (RBL-2H3) cells serve as a model to examine the role of elevated internal Ca2+ concentration ([Ca2+]i), following antigen (DNP10BSA)-induced stimulation of leukotriene C4 (LTC4) formation. A novel action of hydrocortisone (HC), to reduce increased [Ca2+]i and consequently inhibit LTC4 formation is assessed. Half-maximal time for elevation of ... More
Cytosolic and intranuclear calcium signals in rat basophilic leukemia cells as revealed by a confocal fluorescence microscope.
A confocal fluorescence microscope with an argon-ion laser (488 nm) and a He-Cd laser (325 nm) was used to study spatial heterogeneity of the calcium signals in rat basophilic leukemia 2H3 cloned cell line (RBL-2H3). After stimulation with antigen (2,4-dinitrophenol-conjugated bovine serum albumin), fluo-3-fluorescence intensities increased in individual RBL-2H3 cells ... More
Transient translocation and activation of protein phosphatase 2A during mast cell secretion.
AuthorsLudowyke RI, Holst J, Mudge LM, Sim AT
JournalJ Biol Chem
PubMed ID10692405
Okadaic acid inhibits secretion from mast cells, suggesting a regulatory role for protein Ser/Thr phosphatases type I (PP1) and/or 2A (PP2A) in the secretory process. In unstimulated RBL-2H3 cells, okadaic acid pretreatment inhibited PP2A activity in both cytosol and membrane fractions, but inhibition of secretion correlated with inhibition of membrane-bound ... More
Sensitized mast cells migrate toward the antigen: a response regulated by p38 mitogen-activated protein kinase and Rho-associated coiled-coil-forming protein kinase.
AuthorsIshizuka T, Okajima F, Ishiwara M, Iizuka K, Ichimonji I, Kawata T, Tsukagoshi H, Dobashi K, Nakazawa T, Mori M
JournalJ Immunol
PubMed ID11490018
Although mast cells accumulate within the mucosal epithelial layer of patients with allergic rhinitis and bronchial asthma, the responsible chemotactic factors are undefined. We investigated whether mast cells sensitized with Ag-specific IgE migrate toward the Ag. MC/9 mast cells sensitized with anti-DNP IgE migrated toward DNP-conjugated human serum albumin. This ... More
Calcium-independent phosphoinositide breakdown in rat basophilic leukemia cells. Evidence for an early rise in inositol 1,4,5-trisphosphate which precedes the rise in other inositol phosphates and in cytoplasmic calcium.
AuthorsPribluda VS, Metzger H
JournalJ Biol Chem
PubMed ID3040702
Aggregation of the receptor with high affinity for immunoglobulin E (IgE) in rat basophilic leukemia cells leads to a calcium-dependent and a calcium-independent hydrolysis of phosphoinositides. The increase in the levels of inositol phosphates induced in the absence of calcium is only 25% of that observed with 1 mM Ca2+. ... More
Roles of leukotrienes in two rat allergic inflammatory models; IgE-mediated and IgG-antigen complex-induced pleurisies.
AuthorsTanaka K, Ueno A, Katori M
JournalProstaglandins
PubMed ID3020615
Rat IgE pleurisy was induced by the injection of di-nitrophenol-conjugated bovine serum albumin (DNP-BSA) 48 hours after the intrapleural injection of rat anti-DNP-IgE serum. IgG-BSA complex pleurisy was also induced by the intrapleural injection of IgG-BSA complexes produced at the optimum ratio in vitro. Plasma exudation was markedly increased in ... More
p110beta and p110delta phosphatidylinositol 3-kinases up-regulate Fc(epsilon)RI-activated Ca2+ influx by enhancing inositol 1,4,5-trisphosphate production.
AuthorsSmith AJ, Surviladze Z, Gaudet EA, Backer JM, Mitchell CA, Wilson BS
JournalJ Biol Chem
PubMed ID11279065
Fc(epsilon)RI-induced Ca2+ signaling in mast cells is initiated by activation of cytosolic tyrosine kinases. Here, in vitro phospholipase assays establish that the phosphatidylinositol 3-kinase (PI 3-kinase) lipid product, phosphatidylinositol 3,4,5-triphosphate, further stimulates phospholipase Cgamma2 that has been activated by conformational changes associated with tyrosine phosphorylation or low pH. A microinjection ... More
Photobleaching recovery studies of antigen-specific mouse lymphocyte stimulation by DNP-conjugated polymerized flagellin.
AuthorsPeacock JS, Barisas BG
JournalJ Immunol
PubMed ID6167632
We have used fluorescence photobleaching recovery (FPR) to study the lateral diffusion of antigen-receptor complexes during stimulation of DNP-specific mouse B cells by the T-independent antigens DNP-polymerized flagellin (DNP-POL). Depending on epitope density and dose, these antigens behave either as immunogens or tolerogens. Lymphocyte DNP receptors binding DNP0.5 flagellin monomer ... More
Nitric oxide synthesis in rat peritoneal macrophages is induced by IgE/DNP complexes and cyclic AMP analogues. Evidence in favor of a common signaling mechanism.
AuthorsAlonso A, Carvalho J, Alonso-Torre SR, Núñez L, Boscá L, Sánchez Crespo M
JournalJ Immunol
PubMed ID7539021
The production of nitric oxide was studied in rat adherent peritoneal cells stimulated with preformed IgE/DNP-BSA complexes made of IgE obtained from a secreting hybridoma. Stimulation with complexes at equivalence induced both the production of NO and an increased expression of the mRNA of the inducible isoform of NO synthase ... More
The rise in concentration of free Ca2+ and of pH provides sequential, synergistic signals for secretion in antigen-stimulated rat basophilic leukemia (RBL-2H3) cells.
AuthorsAli H, Collado-Escobar DM, Beaven MA
JournalJ Immunol
PubMed ID2794509
Ag stimulation of rat basophilic leukemia (RBL-2H3) cells results in hydrolysis of inositol phospholipids, a transient increase in concentration of cytosol Ca2+ [( Ca2+]i), a gradual increase in cytosolic pH (pHi) and the activation of protein kinase C. To determine whether all these changes serve as signals for secretion, studies ... More
Activation of the mitogen-activated protein kinase cascade is suppressed by low concentrations of dexamethasone in mast cells.
AuthorsRider LG, Hirasawa N, Santini F, Beaven MA
JournalJ Immunol
PubMed ID8805635
Antigen stimulation of mast cells via the IgE receptor, Fc epsilon RI, results in recruitment of the cytosolic tyrosine kinases, Lyn and Syk, and the phosphorylation of proteins. We examined the effects of the glucocorticoid dexamethasone on these events in a cultured (RBL-2H3) mast cell line. Nanomolar concentrations of dexamethasone ... More
Wortmannin blocks lipid and protein kinase activities associated with PI 3-kinase and inhibits a subset of responses induced by Fc epsilon R1 cross-linking.
AuthorsBarker SA, Caldwell KK, Hall A, Martinez AM, Pfeiffer JR, Oliver JM, Wilson BS
JournalMol Biol Cell
PubMed ID8534912
We have investigated the effects of wortmannin, an inhibitor of phosphatidylinositol 3-kinase (PI 3-kinase), on antigen-mediated signaling in the RBL-2H3 mast cell model. In RBL-2H3 cells, the cross-linking of high affinity IgE receptors (Fc epsilon R1) activates at least two cytoplasmic protein tyrosine kinases, Lyn and Syk, and stimulates secretion, ... More
Isoprenoid pathway activity is required for IgE receptor-mediated, tyrosine kinase-coupled transmembrane signaling in permeabilized RBL-2H3 rat basophilic leukemia cells.
AuthorsDeanin GG, Pfeiffer JR, Cutts JL, Fore ML, Oliver JM
JournalCell Regul
PubMed ID1777505
Previously, we reported that the isoprenoid pathway inhibitor, lovastatin, blocks the activation by IgE receptor cross-linking of 45Ca2+ influx, 1,4,5-inositol trisphosphate production, secretion, and membrane changes (ruffling, spreading) in intact RBL-2H3 rat basophilic leukemia cells. These results indicated that an isoprenoid pathway intermediate, very likely an isoprenylated protein, is importantly ... More
The kinetics of phosphoinositide hydrolysis in rat basophilic leukemia (RBL-2H3) cells varies with the type of IgE receptor cross-linking agent used.
AuthorsCunha-Melo JR, Dean NM, Moyer JD, Maeyama K, Beaven MA
JournalJ Biol Chem
PubMed ID3040703
We have re-examined, by high pressure liquid chromatographic (HPLC) procedures, the hydrolysis of 3H-labeled inositol phospholipids in rat basophilic leukemia (RBL-2H3) cells. Previous studies showed no clear correlation between the release of any particular inositol metabolite and the calcium signal in these cells. Paradoxically no responses were observed when the ... More
Distinct functions of the Fc epsilon R1 gamma and beta subunits in the control of Fc epsilon R1-mediated tyrosine kinase activation and signaling responses in RBL-2H3 mast cells.
AuthorsWilson BS, Kapp N, Lee RJ, Pfeiffer JR, Martinez AM, Platt Y, Letourneur F, Oliver JM
JournalJ Biol Chem
PubMed ID7876149
In RBL-2H3 rat tumor mast cells, cross-linking the high affinity IgE receptor, Fc epsilon R1, activates the protein-tyrosine kinases Lyn and Syk and initiates a series of responses including protein-tyrosine phosphorylation, inositol 1,4,5-trisphosphate synthesis, Ca2+ mobilization, secretion, membrane ruffling, and actin plaque assembly. The development of chimeric receptors containing cytoplasmic ... More
Membrane and cytoskeletal changes associated with IgE-mediated serotonin release from rat basophilic leukemia cells.
AuthorsPfeiffer JR, Seagrave JC, Davis BH, Deanin GG, Oliver JM
JournalJ Cell Biol
PubMed ID2933414
Binding of antigen to IgE-receptor complexes on the surface of RBL-2H3 rat basophilic leukemia cells is the first event leading to the release of cellular serotonin, histamine, and other mediators of allergic, asthmatic, and inflammatory responses. We have used dinitrophenol-conjugated bovine serum albumin (DNP-BSA) as well as the fluorescent antigen, ... More
Role of fibronectin on the clearance and tissue uptake of antigen and immune complexes in rats.
AuthorsCosio FG, Bakaletz AP
JournalJ Clin Invest
PubMed ID3316274
In the present study, we have evaluated how plasma fibronectin (FN) and tissue FN can affect the clearance from the circulation and organ uptake of antigen or immune complexes (IC) that have the capacity to bind to FN. Phenylated gelatin (DNP-GL) (a FN binding antigen) and IC composed of DNP-GL ... More
Thymus-dependent network responses to a monoclonal cross-reactive antiidiotypic antibody.
AuthorsBaskin JG, Vakil M, Kearney JF, Ryan T, Lamon EW
JournalJ Immunol
PubMed ID1716282
BALB/c mice were inoculated i.p. with a cross-reactive anti-Idiotypic mAb (designated FD5-1) in the absence of Ag or adjuvants. Injection with unmodified FD5-1 resulted in the induction of serum antibodies reactive with both FD5-1 (Ab3) and the hapten DNP (Ab1'). Endpoint titers of the Ab3 response showed an increase in ... More
High resolution mapping of mast cell membranes reveals primary and secondary domains of Fc(epsilon)RI and LAT.
AuthorsWilson BS, Pfeiffer JR, Surviladze Z, Gaudet EA, Oliver JM
JournalJ Cell Biol
PubMed ID11489921
In mast cells, cross-linking the high-affinity IgE receptor (Fc(epsilon)RI) initiates the Lyn-mediated phosphorylation of receptor ITAMs, forming phospho-ITAM binding sites for Syk. Previous immunogold labeling of membrane sheets showed that resting Fc(epsilon)RI colocalize loosely with Lyn, whereas cross-linked Fc(epsilon)RI redistribute into specialized domains (osmiophilic patches) that exclude Lyn, accumulate Syk, ... More
Extracellular ATP activates mast cells via a mechanism that is different from the activation induced by the cross-linking of Fc receptors.
AuthorsSudo N, Tanaka K, Koga Y, Okumura Y, Kubo C, Nomoto K
JournalJ Immunol
PubMed ID8621938
In this study, the extracellular ATP (ATPo)-induced biochemical events were elucidated by comparing them with either the Fc epsilon RI- or Fc gamma R-induced events in the mouse mast cell line MC9. The omission of extracellular Ca2+ almost completely abolished the elevation of intracellular Ca2+ ([Ca2+]i) in the ATPo-stimulated cells, ... More
Effect of C2-associated carbohydrate structure on Ig effector function: studies with chimeric mouse-human IgG1 antibodies in glycosylation mutants of Chinese hamster ovary cells.
AuthorsWright A, Morrison SL
JournalJ Immunol
PubMed ID9531299
The complex biantennary oligosaccharide at Asn297 of IgG is essential for some effector functions. To investigate the effect of carbohydrate structure on Ab function, we have now expressed mouse-human chimeric IgG1 Abs in Chinese hamster ovary (CHO) cells with defined defects in carbohydrate biosynthesis. We had previously shown that IgG1 ... More
Observing FcepsilonRI signaling from the inside of the mast cell membrane.
AuthorsWilson BS, Pfeiffer JR, Oliver JM
JournalJ Cell Biol
PubMed ID10831616
We have determined the membrane topography of the high-affinity IgE receptor, FcstraightepsilonRI, and its associated tyrosine kinases, Lyn and Syk, by immunogold labeling and transmission electron microscopic (TEM) analysis of membrane sheets prepared from RBL-2H3 mast cells. The method of Sanan and Anderson (Sanan, D.A., and R.G.W. Anderson. 1991. J. ... More
Synthetic sulpholipopolysaccharides: novel adjuvants for humoral immune responses.
AuthorsHilgers LA, Snippe H, Jansze M, Willers JM
JournalImmunology
PubMed ID2880796
Referring to the strong immunostimulating activity of combinations of lipophilic agents and dextran sulphate, conjugates with chemical determinants of both types of adjuvants were synthesized and then examined for immunostimulatory capabilities in mice. Saturated fatty acids with varying chain lengths and sulphate groups were coupled covalently at defined ratios to ... More
Altered patterns of tyrosine phosphorylation and Syk activation for sterically restricted cyclic dimers of IgE-Fc epsilonRI.
AuthorsHarris NT, Goldstein B, Holowka D, Baird B
JournalBiochemistry
PubMed ID9047325
Previous studies in our laboratory established that the symmetrical bivalent ligand, N,N'-bis-[[epsilon-(2,4-dinitrophenyl)amino]caproyl]-L-tyrosyl]-L-cystin e ((DCT)2-cys), stably cross-links anti-2,4-dinitrophenyl-immunoglobulin E (IgE) bound to high affinity receptors Fc epsilonRI on the surface of RBL-2H3 cells, forming mostly cyclic dimers containing two IgE-Fc epsilonRI and two (DCT)2-cys (Posner et al. (1995) J. Immunol. 155, ... More
Importance of bicarbonate ion for intracellular pH regulation in antigen- and ionomycin-stimulated RBL-2H3 mast cells.
AuthorsLee RJ, Oliver JM, Deanin GG, Troup CD, Stump RF
JournalCytometry
PubMed ID1547661
In RBL-2H3 rat basophilic leukemia cells, Ca2+ influx and secretion are activated by antigens that crosslink IgE-receptor complexes and by the Ca2+ ionophore, ionomycin. Here we report that antigen-stimulated Ca2+ influx and secretion are impaired and ionomycin-induced responses are strongly inhibited following the removal of HCO3- from the medium. These ... More
Photobleaching recovery studies of T-independent antigen mobility on antibody-bearing liposomes.
AuthorsPeacock JS, Barisas BG
JournalJ Immunol
PubMed ID6196406
A physicochemical model for the antigen-specific B cell membrane was prepared by incorporating palmitate-conjugated MOPC 315, an anti-DNP IgA, into 8-micron diameter liposomes prepared from phosphatidylcholine, cholesterol, and cardiolipin (2:2:1). With this model system, we examined how the purely passive cross-linking of membrane-bound immunoglobulin molecules by antigen affects the lateral ... More
Identification of variants of the basophilic leukemia (RBL-2H3) cells that have defective phosphoinositide responses to antigen and stimulants of guanosine 5'-triphosphate-regulatory proteins.
AuthorsWoldemussie E, Ali H, Takaishi T, Siraganian RP, Beaven MA
JournalJ Immunol
PubMed ID2443565
Antigen immunoglobulin E-mediated secretion of histamine from RBL-2H3 cells is associated with substantial hydrolysis of membrane inositol phospholipids and a rise in the concentration of cytosol Ca2+ (calcium signal). Such responses differed among cloned variant lines of the RBL-2H3 cell line from undetectable (1A3 bromodeoxyuridine-resistant (BUDRR), 2B1 BUDRR, and 1B3 ... More
Fc epsilon RI-mediated association of 6-micron beads with RBL-2H3 mast cells results in exclusion of signaling proteins from the forming phagosome and abrogation of normal downstream signaling.
AuthorsPierini L, Holowka D, Baird B
JournalJ Cell Biol
PubMed ID8830772
Cells of the mucosal mast cell line, RBL-2H3, are normally stimulated to degranulate after aggregation of high affinity receptors for IgE (Fc epsilon RI) by soluble cross-linking ligands. This cellular degranulation process requires sustained elevation of cytoplasmic Ca2+. In this study, we investigated the response of RBL-2H3 cells to 6-micron ... More
Overcoming the signaling defect of Lyn-sequestering, signal-curtailing FcepsilonRI dimers: aggregated dimers can dissociate from Lyn and form signaling complexes with Syk.
AuthorsLara M, Ortega E, Pecht I, Pfeiffer JR, Martinez AM, Lee RJ, Surviladze Z, Wilson BS, Oliver JM
JournalJ Immunol
PubMed ID11591756
Clustering the tetrameric (alphabetagamma(2)) IgE receptor, FcepsilonRI, on basophils and mast cells activates the Src-family tyrosine kinase, Lyn, which phosphorylates FcepsilonRI beta and gamma subunit tyrosines, creating binding sites for the recruitment and activation of Syk. We reported previously that FcepsilonRI dimers formed by a particular anti-FcepsilonRI alpha mAb (H10) ... More
Roles for Ca2+ stores release and two Ca2+ influx pathways in the Fc epsilon R1-activated Ca2+ responses of RBL-2H3 mast cells.
AuthorsLee RJ, Oliver JM
JournalMol Biol Cell
PubMed ID7579697
Cross-linking the high affinity IgE receptor, Fc epsilon R1, with multivalent antigen induces inositol 1,4,5-trisphosphate [Ins(1,4,5)P3]-dependent release of intracellular Ca2+ stores, Ca2+ influx, and secretion of inflammatory mediators from RBL-2H3 mast cells. Here, fluorescence ratio imaging microscopy was used to characterize the antigen-induced Ca2+ responses of single fura-2-loaded RBL-2H3 cells ... More
Impact of actin rearrangement and degranulation on the membrane structure of primary mast cells: a combined atomic force and laser scanning confocal microscopy investigation.
Authors
JournalBiophys J
PubMed ID19217878
High-resolution three-dimensional imaging of the rich membrane structures of bone marrow-derived mast cells.
Authors
JournalUltramicroscopy
PubMed ID18790570
Unperturbed Immune Function despite Mutation of C-Terminal Tyrosines in Syk Previously Implicated in Signaling and Activity Regulation.
Authors
JournalMol Cell Biol
PubMed ID28760774
Multi-color quantum dot tracking using a high-speed hyperspectral line-scanning microscope.
Authors
JournalPLoS One
PubMed ID23717596
Fluorogen-activating proteins provide tunable labeling densities for tracking FcεRI independent of IgE.
Authors
JournalACS Chem Biol
PubMed ID25343439
ELKS1 controls mast cell degranulation by regulating the transcription of Stxbp2 and Syntaxin 4 via Kdm2b stabilization.
Authors
JournalSci Adv
PubMed ID32937583
Differential mast cell outcomes are sensitive to FcεRI-Syk binding kinetics.
Authors
JournalMol Biol Cell
PubMed ID28855374
Combinatorial diversity of Syk recruitment driven by its multivalent engagement with FcεRIγ.
Authors
JournalMol Biol Cell
PubMed ID31216232
Clathrin Assembly Defines the Onset and Geometry of Cortical Patterning.
Authors
JournalDev Cell
PubMed ID29161594
Cell-based phenotypic screening of mast cell degranulation unveils kinetic perturbations of agents targeting phosphorylation.