Invitrogen™

APO-BrdU™ TUNEL Assay Kit, with Alexa Fluor™ 488 Anti-BrdU

Catalog number: A23210
Invitrogen™

APO-BrdU™ TUNEL Assay Kit, with Alexa Fluor™ 488 Anti-BrdU

Catalog number: A23210
Catalog Number
A23210
Unit Size
60 assays
Price (USD)
Price: 710.00
Your Price:
Availability
-
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
A2321060 assays
Price: 710.00
Your Price:
-
Product Overview
Figures
Videos
Recommendations
Recommendations
Documents
FAQ
Citations & References
Additional Information
Recommendations
The APO‑BrdU TUNEL Assay Kit provides the materials necessary to label DNA strand breaks for the detection of apoptotic cells, as well as fixed samples of positive and negative control cells for assessing assay performance. Complete protocols for flow cytometry applications are also included, although the kit may be adapted for use with fluorescence microscopy as well. Final detection of BrdU incorporation at DNA break sites is achieved through an Alexa Fluor 488 dye–labeled anti‑BrdU antibody. The kit also includes propidium iodide to determine the total cellular DNA content.

The APO‑BrdU TUNEL Assay Kit detects the DNA fragmentation of apoptotic cells by exploiting the fact that the DNA breaks expose a large number of 3´‑hydroxyl ends. These hydroxyl groups can then serve as starting points for terminal deoxynucleotidyl transferase (TdT), which adds deoxyribonucleotides in a template-independent fashion. Addition of the deoxythymidine analog 5‑bromo‑2´‑deoxyuridine 5´‑triphosphate (BrdUTP) to the TdT reaction serves to label the break sites. Once incorporated into the DNA, BrdU can be detected by an anti‑BrdU antibody using standard immunohistochemical techniques. This method of labeling DNA breaks is referred to as Terminal Deoxynucleotide Transferase dUTP Nick End Labeling, or TUNEL.


For Research Use Only. Not for use in diagnostic procedures.

Specifications

Color
Green
Detection Method
Fluorescent
Format
Tube
Excitation/Emission
490/525
Flow Cytometer Laser Lines
488
For Use With (Application)
Flow Cytometry
For Use With (Equipment)
Fluorescence Microscope, Flow Cytometer
Label or Dye
Alexa Fluor™ 488
Product Type
TUNEL Assay Kit
Product Line
APO-BrdU™
Quantity
60 Assays
Species
Mouse
Target
BrdU
Label Type
Alexa Fluor Dyes

Contents & Storage

Contains positive control cells (5 mL) and negative control cells (5 mL) of a fixed human lymphoma cell line, 1 vial of TdT (45 µL), 1 vial of BrdUTP (480 µL), 1 vial of anti-BrdU Ms mAb Alexa Fluor™ 488 conjugate (350 µL), 1 bottle of PI/Rnase A staining buffer (30 mL), 1 vial of reaction buffer (0.6 mL), 1 bottle of wash buffer (120 mL) and 1 bottle of rinse buffer (120 mL).

Store as directed.