APO-BrdU™ TUNEL Assay Kit, with Alexa Fluor™ 488 Anti-BrdU

Catalog number:  A23210

Invitrogen™  Related applications: Cell Viability, Proliferation & Function | Cellular Imaging | Chromatin Biology | Flow Cytometry

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Description

In collaboration with Phoenix Flow Systems, we offer the APO™-BrdU TUNEL Assay Kit which provides all the materials necessary to label and detect the DNA strand breaks of apoptotic cells. When DNA strands are cleaved or nicked by nucleases, a large number of 3´hydroxyl ends are exposed. In the APO-BrdU assay, these ends are labeled with BrdUTP and terminal deoxynucleotidyl transferase (TdT) using the TUNEL technique. Once incorporated ino the DNA, BrdU is detected using a bright and photostable green-fluorescent Alexa Fluor™ 488 dye-labeled anti-BrdU antibody.

View a selection guide for all apoptosis assays for flow cytometry.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Detection Method: Fluorescent
Excitation⁄Emission (nm): 495⁄519
For Use With (Equipment): Flow Cytometer, Fluorescence Microscope
Format: Tube(s)
Label or Dye: Alexa Fluor™ 488
Number of Reactions: 60
Product Line: APO-BrdU™
Product Size: 60 assays
Target Gene: BrdU

Contents & storage

Contains positive control cells (5 mL) and negative control cells (5 mL) of a fixed human lymphoma cell line, 1 vial of TdT (45 µL), 1 vial of BrdUTP (480 µL), 1 vial of anti-BrdU Ms mAb Alexa Fluor® 488 conjugate (350 µL), 1 bottle of PI/Rnase A staining buffer (30 mL), 1 vial of reaction buffer (0.6 mL), 1 bottle of wash buffer (120 mL) and 1 bottle of rinse buffer (120 mL).

Store as directed.

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