Invitrogen™

APO-BrdU™ TUNEL Assay Kit, with Alexa Fluor™ 488 Anti-BrdU

Catalog number: A23210
Invitrogen™

APO-BrdU™ TUNEL Assay Kit, with Alexa Fluor™ 488 Anti-BrdU

Catalog number: A23210
Catalog Number
A23210
Unit Size
60 assays
Price (USD)
650.00
Your Price:
Availability
***
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
A2321060 assays
650.00
Your Price:
***
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In collaboration with Phoenix Flow Systems, we offer the APO™-BrdU TUNEL Assay Kit which provides all the materials necessary to label and detect the DNA strand breaks of apoptotic cells. When DNA strands are cleaved or nicked by nucleases, a large number of 3´hydroxyl ends are exposed. In the APO-BrdU assay, these ends are labeled with BrdUTP and terminal deoxynucleotidyl transferase (TdT) using the TUNEL technique. Once incorporated ino the DNA, BrdU is detected using a bright and photostable green-fluorescent Alexa Fluor™ 488 dye-labeled anti-BrdU antibody.

View a selection guide for all apoptosis assays for flow cytometry.

Specifications

Color
Green
Detection Method
Fluorescent
Format
Tube
Excitation/Emission
490/525
Flow Cytometer Laser Lines
488
For Use With (Application)
Flow Cytometry
For Use With (Equipment)
Fluorescence Microscope, Flow Cytometer
Label or Dye
Alexa Fluor™ 488
Product Type
TUNEL Assay Kit
Product Line
APO-BrdU™
Quantity
60 Assays
Species
Mouse
Target
BrdU
Label Type
Alexa Fluor Dyes

Contents & Storage

Contains positive control cells (5 mL) and negative control cells (5 mL) of a fixed human lymphoma cell line, 1 vial of TdT (45 µL), 1 vial of BrdUTP (480 µL), 1 vial of anti-BrdU Ms mAb Alexa Fluor™ 488 conjugate (350 µL), 1 bottle of PI/Rnase A staining buffer (30 mL), 1 vial of reaction buffer (0.6 mL), 1 bottle of wash buffer (120 mL) and 1 bottle of rinse buffer (120 mL).

Store as directed.

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